lovely density - i would like to know what % of series have multiple
conformations - it would need a survey I guess of PDB depositions, not just
coordinates, but checking maps..
Eleanor
On 21 May 2012 22:21, Uma Ratu rosiso2...@gmail.com wrote:
Thank you All for you inputs.
Uma
On Mon,
Dear Uma
1. The protein sequence given in the databases, do from time to time
have errors, particularly if you are working with old proteins (when
the sequencing was done a long time ago). What you observe could also be
a threonine or even a valine. You should check whether homologous
3. Of course if you are working on recombinant protein, you should
double check the sequencing results from the cloning
Preben
On 5/21/12 11:21 PM, Uma Ratu wrote:
Thank you All for you inputs.
Uma
On Mon, May 21, 2012 at 5:06 PM, Van Den Berg, Bert
lambertus.vandenb...@umassmed.edu
I believe that's an alternative conformationtry modeling as such and
see what happens.
Jacob
On Mon, May 21, 2012 at 3:57 PM, Uma Ratu rosiso2...@gmail.com wrote:
Dear All:
Some of serine residues in my model have extra positive Fo-Fc density at
the edge of side chain. Some don't have.
Yes, as Jacob says, alternative conformation of the serine. Quite common.
Bert
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Uma Ratu
[rosiso2...@gmail.com]
Sent: Monday, May 21, 2012 4:57 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Serine
Thank you All for you inputs.
Uma
On Mon, May 21, 2012 at 5:06 PM, Van Den Berg, Bert
lambertus.vandenb...@umassmed.edu wrote:
Yes, as Jacob says, alternative conformation of the serine. Quite common.
Bert
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*From:* CCP4 bulletin board
