Feb 2013 16:14:58 -0600
From: CCP4 bulletin board CCP4BB@JISCMAIL.AC.UK (on behalf of Artem
Evdokimov artem.evdoki...@gmail.com)
Subject: Re: [ccp4bb] Sighting of Protein Crystals in Vivo?!
To: CCP4BB@JISCMAIL.AC.UK
Note: the crystals in my paper are not regular GFP,
they're special GFP from
Jacob, I recall Dorothy Hodgkin or Guy Dodson showing a slide (mid to late
1970s) with insulin crystals having grown in the Islets of Langerhans. As you
say, quite remarkable. John
Prof John R Helliwell DSc
On 15 Feb 2013, at 19:44, Jacob Keller j-kell...@fsm.northwestern.edu wrote:
On 02/16/13 00:46, Zhijie Li wrote:
Hi Jacob,
Interesting topic.
This reminds me the posters I saw on ACA 2010, on the femto-second
infrared laser based instrument . That instrument utilizes the
nonlinear optical properties of crystals of chiral molecules to
detect very small crystalline
In addition to the above mentioned references you can also see:
Charcot-Leyden crystals (http://www.ncbi.nlm.nih.gov/pubmed/6508005)
and my own ( :) ) figure 1
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1618374/figure/f1/
Cheers,
Artem
On Fri, Feb 15, 2013 at 11:46 PM, Zhijie Li
Original message
Date: Sat, 16 Feb 2013 15:38:12 -0600
From: CCP4 bulletin board CCP4BB@JISCMAIL.AC.UK (on behalf of Artem
Evdokimov artem.evdoki...@gmail.com)
Subject: Re: [ccp4bb] Sighting of Protein Crystals in Vivo?!
To: CCP4BB@JISCMAIL.AC.UK
In addition to the above
Hi Jacob,
check out Figure 1 in
Natively inhibited Trypanosoma brucei cathepsin B structure determined by using
an X-ray laser.
Redecke L, et al.
Science. 2013 Jan 11;339(6116):227-30.
and
In vivo protein crystallization opens new routes in structural biology.
Koopmann R. Nature Methods.
Of course, common in baculo:
http://dx.doi.org/10.1038/emboj.2009.352
The EMBO Journal (2010) 29,505--514
*How baculovirus polyhedra fit square pegs into round holes to robustly
package viruses*
Xiaoyun Ji, Geoff Sutton, Gwyndaf Evans, Danny Axford, Robin Owen and
David I Stuart
On
Hi Jacob,
They are not too small to mount I think, at least 20 microns long (compared to
the size of surrounding cells). But what do you mean by littered? There seems
to be just one cell with crystals out of say 10 expressing eGFP. And why are
there only 10 or so expressing GFP out of ~200 or
://hmg.oxfordjournals.org/content/9/12/1779.long
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Frank von
Delft
Sent: Friday, February 15, 2013 3:01 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Sighting of Protein Crystals in Vivo?!
Of course
: Re: [ccp4bb] Sighting of Protein Crystals in Vivo?!
Of course, common in baculo:
http://dx.doi.org/10.1038/emboj.2009.352
The EMBO Journal (2010) 29,505–514
How baculovirus polyhedra fit square pegs into round holes to robustly package
viruses
Xiaoyun Ji, Geoff Sutton, Gwyndaf Evans
Hi Jacob,
Interesting topic.
This reminds me the posters I saw on ACA 2010, on the femto-second infrared
laser based instrument . That instrument utilizes the nonlinear optical
properties of crystals of chiral molecules to detect very small crystalline
materials from amorphous background:
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