My default MAD strategy is to do single-image inverse beam with round
robin wavelength changes. That is:
energy phi
peak 0
peak 180
remote 0
remote 180
peak1
peak 181
remote1
etc
with one image taken for each line above. I do this until a full
sphere is
Yafang,
I'm afraid that just because you still have spots at the end of your
dataset does not mean radiation damage was not a problem. The
reactions that disorder your heavy atom sites go to completion at doses
that can be as little as 1/30th of the dose required to noticeably fade
your
Dear Yafang,
If radiation damage is not a major problem, MAD should give you more
phase information than SAD, i.e. better maps, especially
at low resolution. If SAD works but MAD doesn't, there are several
possible explanations:
1. (most likely) your datasets are inconsistently indexed. This
Dear George,
if #4 is correct, shouldn't he be able to get good SIRAS using the peak dataset
as HA and the last collected dataset as native
Jürgen
..
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry Molecular Biology
Johns
