Re: [ccp4bb] protein quasicrystals?

2018-02-16 Thread Harry Powell
> > > > From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Joseph > Ferrara > Sent: Thursday, February 15, 2018 4:57 PM > To: CCP4BB@JISCMAIL.AC.UK > Subject: Re: [ccp4bb] protein quasicrystals? > > Nakane-san, > > There is also Crys

Re: [ccp4bb] protein quasicrystals?

2018-02-15 Thread Richard Staples
board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Joseph Ferrara Sent: Thursday, February 15, 2018 4:57 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] protein quasicrystals? Nakane-san, There is also CrysAlisPro, which can handle multiple crystals and may already support your detector.

Re: [ccp4bb] protein quasicrystals?

2018-02-15 Thread Joseph Ferrara
, 2018 2:03 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] protein quasicrystals? There are programs which are good at indexing patterns from multiply twinned crystals. Bruker AXS has one, to my knowledge. There may be other sources. I suggest you try that first before you invoke a quasicrystal

Re: [ccp4bb] protein quasicrystals?

2018-02-15 Thread Satya
Qiu <yu@sanofi.com> wrote: > >> As asked by a few people, here are the images of crystals and diffraction. >> >> >> >> Thanks, >> >> Yu >> >> >> >> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf

Re: [ccp4bb] protein quasicrystals?

2018-02-14 Thread Prem Prakash
Hi Yu, Microseeding along with additive screening could give a better result, because I encountered a situation once like this. Try higher concentration of protein with bigger drop volume during crystallisation. Good luck Prem On Wed, Feb 14, 2018 at 4:16 PM, Huyton, Trevor

Re: [ccp4bb] protein quasicrystals?

2018-02-14 Thread Huyton, Trevor
Dear Yu, If the crystals are grown from salt then put the drop over water for a few hours and dissolve them, then put it back over the reservoir solution and recrystallize. Sometimes you can get better crystals this way. You could also try melting them with temperature and recrystallizing Best

Re: [ccp4bb] protein quasicrystals?

2018-02-13 Thread Gyanendra Kumar
Hi Yu, Have you tried using the Additive Screen (e.g. from Hampton Research) on top of your crystallization condition, and varying the ratio between protein complex and crystallization solution? From the diffraction pattern, it looks you are almost there. Salt crystals don't show spots close to

Re: [ccp4bb] protein quasicrystals?

2018-02-13 Thread Diana Tomchick
nofi.com<mailto:yu@sanofi.com> [mailto:yu@sanofi.com] Sent: Tuesday, February 13, 2018 4:03 PM To: Keller, Jacob <kell...@janelia.hhmi.org<mailto:kell...@janelia.hhmi.org>>; CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Subject: RE: [ccp4bb] protein quasicrystals? As

Re: [ccp4bb] protein quasicrystals?

2018-02-13 Thread Keller, Jacob
in the future. From: yu@sanofi.com [mailto:yu@sanofi.com] Sent: Tuesday, February 13, 2018 4:03 PM To: Keller, Jacob <kell...@janelia.hhmi.org>; CCP4BB@JISCMAIL.AC.UK Subject: RE: [ccp4bb] protein quasicrystals? As asked by a few people, here are the images of crystals and diffr

Re: [ccp4bb] protein quasicrystals?

2018-02-13 Thread Keller, Jacob
to this message and follow with its deletion, so that we can ensure such a mistake does not occur in the future. From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of James Phillips Sent: Tuesday, February 13, 2018 3:03 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] protein quasicrystals

Re: [ccp4bb] protein quasicrystals?

2018-02-13 Thread James Phillips
There are programs which are good at indexing patterns from multiply twinned crystals. Bruker AXS has one, to my knowledge. There may be other sources. I suggest you try that first before you invoke a quasicrystal explanation. James Phillips On Tue, Feb 13, 2018 at 12:07 PM, Takanori Nakane

Re: [ccp4bb] protein quasicrystals?

2018-02-13 Thread Takanori Nakane
Hi, "dials.reciprocal_space_viewer" is very useful to identify multiple lattices. For quasicrystal and modulated crystals, "dials.rs_mapper" is also very useful. Best regards, Takanori Nakane > Have you tried microseeding of these sphere crystals? It may help to get > better crystals. > > >

Re: [ccp4bb] protein quasicrystals?

2018-02-13 Thread Kabasakal, Burak V
Have you tried microseeding of these sphere crystals? It may help to get better crystals. Burak From: CCP4 bulletin board on behalf of Yu Qiu Sent: 13 February 2018 15:09:43 To: CCP4BB@JISCMAIL.AC.UK Subject:

Re: [ccp4bb] protein quasicrystals?

2018-02-13 Thread Gloria Borgstahl
I am very interested in this topic, and have found incommensurately modulated crystals and few examples of possible protein quasicrystals. Do you have any images you could share? On Tue, Feb 13, 2018 at 9:09 AM, Yu Qiu wrote: > Hi, > > > > I have been trying to crystallize a