>
>
>
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Joseph
> Ferrara
> Sent: Thursday, February 15, 2018 4:57 PM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] protein quasicrystals?
>
> Nakane-san,
>
> There is also Crys
board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Joseph
Ferrara
Sent: Thursday, February 15, 2018 4:57 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] protein quasicrystals?
Nakane-san,
There is also CrysAlisPro, which can handle multiple crystals and may already
support your detector.
, 2018 2:03 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] protein quasicrystals?
There are programs which are good at indexing patterns from multiply twinned
crystals. Bruker AXS has one, to my knowledge. There may be other sources. I
suggest you try that first before you invoke a quasicrystal
ked by a few people, here are the images of crystals and diffraction.
>>
>>
>>
>> Thanks,
>>
>> Yu
>>
>>
>>
>> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of
>> *Keller, Jacob
>
Hi Yu,
Microseeding along with additive screening could give a better result,
because I encountered a situation once like this. Try higher concentration
of protein with bigger drop volume during crystallisation.
Good luck
Prem
On Wed, Feb 14, 2018 at 4:16 PM, Huyton, Trevor wrote:
> Dear Yu,
Dear Yu,
If the crystals are grown from salt then put the drop over water for a few
hours and dissolve them, then put it back over the reservoir solution and
recrystallize.
Sometimes you can get better crystals this way.
You could also try melting them with temperature and recrystallizing
Best
Hi Yu,
Have you tried using the Additive Screen (e.g. from Hampton Research) on
top of your crystallization condition, and varying the ratio between
protein complex and crystallization solution? From the diffraction pattern,
it looks you are almost there. Salt crystals don't show spots close to th
com> [mailto:yu@sanofi.com]
Sent: Tuesday, February 13, 2018 4:03 PM
To: Keller, Jacob mailto:kell...@janelia.hhmi.org>>;
CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: RE: [ccp4bb] protein quasicrystals?
As asked by a few people, here are the images of crystals and diffra
the future.
From: yu@sanofi.com [mailto:yu@sanofi.com]
Sent: Tuesday, February 13, 2018 4:03 PM
To: Keller, Jacob ; CCP4BB@JISCMAIL.AC.UK
Subject: RE: [ccp4bb] protein quasicrystals?
As asked by a few people, here are the images of crystals and diffraction.
Thanks,
Yu
From: CCP4 bulletin
message and follow with
its deletion, so that we can ensure such a mistake does not occur in the future.
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of James
Phillips
Sent: Tuesday, February 13, 2018 3:03 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] protein quasicrystals
There are programs which are good at indexing patterns from multiply
twinned crystals. Bruker AXS has one, to my knowledge. There may be other
sources. I suggest you try that first before you invoke a quasicrystal
explanation.
James Phillips
On Tue, Feb 13, 2018 at 12:07 PM, Takanori Nakane w
Hi,
"dials.reciprocal_space_viewer" is very useful to identify multiple lattices.
For quasicrystal and modulated crystals, "dials.rs_mapper" is also very
useful.
Best regards,
Takanori Nakane
> Have you tried microseeding of these sphere crystals? It may help to get
> better crystals.
>
>
> Bur
Have you tried microseeding of these sphere crystals? It may help to get better
crystals.
Burak
From: CCP4 bulletin board on behalf of Yu Qiu
Sent: 13 February 2018 15:09:43
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] protein quasicrystals?
Hi,
I have be
I am very interested in this topic, and have found incommensurately
modulated crystals and few examples of possible protein quasicrystals.
Do you have any images you could share?
On Tue, Feb 13, 2018 at 9:09 AM, Yu Qiu wrote:
> Hi,
>
>
>
> I have been trying to crystallize a protein complex and k
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