Re: [ccp4bb] strange X-ray diffraction diagram――RNA Or Complex?

2016-10-12 Thread Petri Kursula
From your picture it looks like you have both these needle clusters and single needle/rod-lke crystals. The diffraction pattern you show is what you expect from a needle cluster (with the ring-like patterns at low resolution), a single needle or a larger optimized single crystal should give you

Re: [ccp4bb] strange X-ray diffraction diagram――RNA Or Complex?

2016-10-12 Thread Mark J van Raaij
Dear Yujie, At first I thought this looked just like diffraction of multiple salt crystals, but there are perhaps a couple of rows of regularly spaced spots from a protein crystals. However, I think any program will struggle to get anything useful from this. Perhaps focussing a small beam

Re: [ccp4bb] strange X-ray diffraction diagram——RNA Or Complex?

2016-10-12 Thread Thomas, Leonard M.
Yujie, I would try using another processing program if you have it available, say Mosflm. I look at similar looking diffraction data recently, though without the salt crystals mixed in as Herman observed which you also need to take care of in the future, and HKL did not handle if very well,