[galaxy-dev] DRMAA Slurm error

2014-09-25 Thread Pardo Diaz, Alfonso
Hi,


I have configured a new galaxy-project site with SLURM (version 14). I have one 
server with a Galaxy-Project instance, one node with SLURM server and two SLURM 
worker nodes. I have compile SLURM-DRMAA from source codes. When I run 
“drmaa-run /bin/hostname” it’s work. But, when I try to run the server I got 
the next error:

Traceback (most recent call last):
  File /home/galaxy-dist/lib/galaxy/webapps/galaxy/buildapp.py, line 39, in 
app_factory
app = UniverseApplication( global_conf = global_conf, **kwargs )
  File /home/galaxy-dist/lib/galaxy/app.py, line 141, in __init__
self.job_manager = manager.JobManager( self )
  File /home/galaxy-dist/lib/galaxy/jobs/manager.py, line 23, in __init__
self.job_handler = handler.JobHandler( app )
  File /home/galaxy-dist/lib/galaxy/jobs/handler.py, line 32, in __init__
self.dispatcher = DefaultJobDispatcher( app )
  File /home/galaxy-dist/lib/galaxy/jobs/handler.py, line 704, in __init__
self.job_runners = self.app.job_config.get_job_runner_plugins( 
self.app.config.server_name )
  File /home/galaxy-dist/lib/galaxy/jobs/__init__.py, line 621, in 
get_job_runner_plugins
rval[id] = runner_class( self.app, runner[ 'workers' ], **runner.get( 
'kwds', {} ) )
  File /home/galaxy-dist/lib/galaxy/jobs/runners/drmaa.py, line 81, in 
__init__
self.ds.initialize()
  File /home/galaxy-dist/eggs/drmaa-0.7.6-py2.6.egg/drmaa/session.py, line 
257, in initialize
py_drmaa_init(contactString)
  File /home/galaxy-dist/eggs/drmaa-0.7.6-py2.6.egg/drmaa/wrappers.py, line 
73, in py_drmaa_init
return _lib.drmaa_init(contact, error_buffer, sizeof(error_buffer))
  File /home/galaxy-dist/eggs/drmaa-0.7.6-py2.6.egg/drmaa/errors.py, line 
151, in error_check
raise _ERRORS[code - 1](error_string)
AlreadyActiveSessionException: code 11: DRMAA session already exist.
[root@galaxy-project galaxy-dist]#


This is my “job_conf.xml”:

job_conf
plugins workers=4
plugin id=local type=runner 
load=galaxy.jobs.runners.local:LocalJobRunner/
plugin id=drmaa type=runner 
load=galaxy.jobs.runners.drmaa:DRMAAJobRunner/
plugin id=cli type=runner 
load=galaxy.jobs.runners.cli:ShellJobRunner /
plugin id=slurm type=runner 
load=galaxy.jobs.runners.slurm:SlurmJobRunner
param id=drmaa_library_path/usr/local/lib/libdrmaa.so/param
/plugin
/plugins
handlers
handler id=main/
/handlers
destinations default=drmaa_slurm
destination id=local runner=local/
destination id=multicore_local runner=local
  param id=local_slots4/param
  param id=embed_metadata_in_jobTrue/param
  job_metrics /
/destination
destination id=docker_local runner=local
  param id=docker_enabledtrue/param
/destination
destination id=drmaa_slurm runner=drmaa
param 
id=galaxy_external_runjob_scriptscripts/drmaa_external_runner.py/param
param 
id=galaxy_external_killjob_scriptscripts/drmaa_external_killer.py/param
param 
id=galaxy_external_chown_scriptscripts/external_chown_script.py/param
/destination
destination id=direct_slurm runner=slurm
param id=nativeSpecification--time=00:01:00/param
/destination
/destinations
resources default=default
  group id=default/group
  group id=memoryonlymemory/group
  group id=allprocessors,memory,time,project/group
/resources
tools
tool id=foo handler=trackster_handler
param id=sourcetrackster/param
/tool
tool id=bar destination=dynamic/
tool id=longbar destination=dynamic resources=all /
tool id=baz handler=special_handlers destination=bigmem/
/tools
limits
limit type=registered_user_concurrent_jobs2/limit
limit type=anonymous_user_concurrent_jobs1/limit
limit type=destination_user_concurrent_jobs id=local1/limit
limit type=destination_user_concurrent_jobs tag=mycluster2/limit
limit type=destination_user_concurrent_jobs tag=longjobs1/limit
limit type=destination_total_concurrent_jobs id=local16/limit
limit type=destination_total_concurrent_jobs 
tag=longjobs100/limit
limit type=walltime24:00:00/limit
limit type=output_size10GB/limit
/limits
/job_conf


Can you help me? I am newbie with “Galaxy Project” administration.




THANKS IN ADVANCE





Alfonso Pardo Diaz
System Administrator / Researcher
c/ Sola nº 1; 10200 Trujillo, ESPAÑA
Tel: +34 927 65 93 17 Fax: +34 927 32 32 37

[CETA-Ciemat logo]http://www.ceta-ciemat.es/



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ha 

Re: [galaxy-dev] problems installing tool dependency

2014-09-25 Thread Sajdak, Doris
Thank you very much Nate and thanks, Will, for helping with the troubleshooting.

Dori


From: Nate Coraor [mailto:n...@bx.psu.edu]
Sent: Wednesday, September 24, 2014 2:58 PM
To: Will Holtz
Cc: Sajdak, Doris; galaxy-dev@lists.bx.psu.edu
Subject: Re: [galaxy-dev] problems installing tool dependency

I've just looked at this as well, it appears that /pub4 is not exposed and does 
not allow you to change into it, but it does allow you to fetch if you request 
files under it via the full path.

e.g.:

nate@victory% ncftp ftp.sanger.ac.ukhttp://ftp.sanger.ac.uk
...
Logged in to ftp.sanger.ac.ukhttp://ftp.sanger.ac.uk.
ncftp /  ls
ls-lR.gz.ok_to_rsyncREADME  
.request-for-update.lock
.messagepub/
.request-for-update
ncftp /  cd pub4
Could not chdir to pub4: server said: pub4: No such file or directory
ncftp /  cd /pub4
Could not chdir to /pub4: server said: pub4: No such file or directory
ncftp /  get /pub4/resources/software/quicktree/quicktree.tar.gz
quicktree.tar.gz:   38.12 kB  124.19 kB/s
ncftp / 

I'll contact some folks in the Miller Lab to see if the tool can be updated to 
the path under /pub.

On Wed, Sep 24, 2014 at 2:09 PM, Will Holtz 
who...@lygos.commailto:who...@lygos.com wrote:
I also was able to download quicktree.tar.gz by clicking on the URL in your 
message. However, when I manually ftp into 
ftp.sanger.ac.ukhttp://ftp.sanger.ac.uk, there is no /pub4 directory. I'm a 
bit confused about how those two statements are both true, but maybe it will 
help someone else figure this out. Removing the '4' does result in a valid path 
(ftp://ftp.sanger.ac.uk/pub/resources/software/quicktree/quicktree.tar.gz).
-Will

On Wed, Sep 24, 2014 at 10:57 AM, Sajdak, Doris 
dj...@buffalo.edumailto:dj...@buffalo.edu wrote:
Hi all,

I am trying to install the Genome Diversity tools from the Miller Lab that are 
in the Galaxy toolshed.  I’ve been able to get all dependencies installed 
except Quicktree.  Whenever I try to install it, I get the error:

Error downloading from URL
ftp://ftp.sanger.ac.uk/pub4/resources/software/quicktree/quicktree.tar.gz:
urlopen error ftp error: 550 pub4: No such file or directory

This is the tool_dependencies.xml file for installing quicktree:

?xml version=1.0?
tool_dependency
  package name=quicktree version=1.1
install version=1.0
  actions
!-- Download source code --
action type=download_by_url 
target_filename=quicktree_1.1.tar.gzftp://ftp.sanger.ac.uk/pub4/resources/software/quicktree/quicktree.tar.gz/action

!-- Build quicktree --
action type=shell_commandmake quicktree/action

!-- Install quicktree --
action type=shell_commandcp -R bin $INSTALL_DIR/action

!-- Set environment for dependent repositories --
action type=set_environment
  environment_variable name=PATH 
action=prepend_to$INSTALL_DIR/bin/environment_variable
/action
  /actions
/install
  /package
/tool_dependency


I know this is not their file I’m downloading but the thing is, if I copy and 
paste the URL, I can download it with no problem.  I’m not sure why it’s 
reporting that it can’t be found but the xml looks right to me.  I’ve tried 
contacting the Miller Lab about this but haven’t heard from anyone so I’m 
hoping someone here can tell me how to move past this problem.  Is there a way 
to install this tool manually and have the main Genome Diversity tools 
recognize it on the Galaxy server?

Also, I noticed when looking at the Tool Dependency Definitions at 
https://toolshed.g2.bx.psu.edu/ there are errors going back many versions for 
this tool (see Test runs – Installation errors – Tool dependencies).

Thank you for any help you can provide.

Dori

**
Dori Sajdak
Senior Systems Administrator
State University of NY at Buffalo
Center for Computational Research
701 Ellicott St
Buffalo, NY 14203
Phone: (716) 881-8934tel:%28716%29%20881-8934
Fax: (716) 849-6656tel:%28716%29%20849-6656
Web: http://ccr.buffalo.edu
**




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Please 

Re: [galaxy-dev] tool for STAR RNA-seq aligner

2014-09-25 Thread David Hoover

Ross,

About the index files:  It is way easier to have pre-built index files.  
However, when running a 2-pass STAR run, a user will need to generate 
their own reference index files based on the output SJ.tab.out file 
created in the first pass.  Is this incorporated into your tool?


About shared memory:  I am under the impression that the latest version 
of STAR has deprecated this feature.  I am unclear how this would help 
unless a single large-memory machine was dedicated to running all STAR 
jobs.  Is this the case?


Also, does the tool merge the SAM/BAM file with the output chimeric SAM 
file?


David Hoover

On 9/24/2014 7:03 PM, Ross wrote:

Hi All,

That (fubar in testtoolshed) star wrapper was derived from one 
originally written by Jeremy Goecks. I modified it for multiple inputs 
and added a few tweaks and it has been in production use in our group 
for about 6 months so I'm pretty sure it works reasonably well in our 
hands at least.


I would really appreciate any available help getting it to a proven 
useful state - suggestions and code welcomed. I have not moved it to 
the main toolshed because aside from some encouragement, I've had no 
feedback to suggest it's working - or not. It is extremely fast - we 
regularly see 200-300M reads per minute in the logs!


We regularly run a whole experiment worth (eg 12 - 24) fastq files 
simultaneously with the shared memory option working on our cluster - 
see the readme.


Star index files made with a gene model (requires valid gff3) are huge 
- 20-30GB for hg19 - hence the need for shared memory if you run 
multiple jobs. That will eventually become a serious problem if you 
really want to allow users to make their own - we definitely do not. 
You need to be very careful about matching the gene model gff3 file to 
the reference and I had enough trouble getting it right for the few 
major genomes we use to make me think that I do not want users trying 
to do that generating 25GB of rubbish every time they get it wrong.


There are challenges to do with needing different indexes for 
different length reads but we are seeing fairly consistent 60bp single 
ended reads for most of the incoming RNA seq experiments.


A data manager would be a boon if anyone cares to write one...


On Thu, Sep 25, 2014 at 6:55 AM, Curtis Hendrickson (Campus) 
curt...@uab.edu mailto:curt...@uab.edu wrote:


Bjorn

We'd be interested in this tool, as well. Any idea how close to
functional it is?
I see it's only on TEST toolshed, and not on production, at this
point.

I don't see any related Trello card when searching on star

Regards,
Curtis
Galaxy Admin @ University of Alabama at Birmingham

-Original Message-
From: galaxy-dev-boun...@lists.bx.psu.edu
mailto:galaxy-dev-boun...@lists.bx.psu.edu
[mailto:galaxy-dev-boun...@lists.bx.psu.edu
mailto:galaxy-dev-boun...@lists.bx.psu.edu] On Behalf Of Björn
Grüning
Sent: Wednesday, September 24, 2014 3:15 PM
To: galaxy-dev@lists.bx.psu.edu
mailto:galaxy-dev@lists.bx.psu.edu; hoove...@helix.nih.gov
mailto:hoove...@helix.nih.gov  David Hoover
Subject: Re: [galaxy-dev] tool for STAR RNA-seq aligner

Hi David,

yes there is inital code in the
https://testtoolshed.g2.bx.psu.edu/. I think Ross has done some
work on it.
The main problem with Star is that is needs special indices (and a
lot of it) and it would be great to offer data managers for it.

Cheers,
Bjoern

Am 24.09.2014 um 22:05 schrieb David Hoover:
 Hi,

 I am developing a tool for STAR
(https://code.google.com/p/rna-star/), and I realize I may be
reinventing another wheel.  Has anyone else created a tool for
STAR?  There's nothing else in the toolsheds for it yet.

 David

 
 David Hoover, PhD
 Helix Systems Staff
 SCB/DCSS/CIT/NIH
 301-435-2986
 http://helix.nih.gov





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Re: [galaxy-dev] DRMAA Slurm error

2014-09-25 Thread Pardo Diaz, Alfonso
Solved!


The problem was I have configured the job_conf.xml two plugins entry with 
DRMAA. I have deleted the entry:

 plugin id=slurm type=runner 
load=galaxy.jobs.runners.slurm:SlurmJobRunner

And now works!


Thanks



El 25/09/2014, a las 08:12, Pardo Diaz, Alfonso 
alfonso.pa...@ciemat.esmailto:alfonso.pa...@ciemat.es escribió:

Hi,


I have configured a new galaxy-project site with SLURM (version 14). I have one 
server with a Galaxy-Project instance, one node with SLURM server and two SLURM 
worker nodes. I have compile SLURM-DRMAA from source codes. When I run 
“drmaa-run /bin/hostname” it’s work. But, when I try to run the server I got 
the next error:

Traceback (most recent call last):
  File /home/galaxy-dist/lib/galaxy/webapps/galaxy/buildapp.py, line 39, in 
app_factory
app = UniverseApplication( global_conf = global_conf, **kwargs )
  File /home/galaxy-dist/lib/galaxy/app.py, line 141, in __init__
self.job_manager = manager.JobManager( self )
  File /home/galaxy-dist/lib/galaxy/jobs/manager.py, line 23, in __init__
self.job_handler = handler.JobHandler( app )
  File /home/galaxy-dist/lib/galaxy/jobs/handler.py, line 32, in __init__
self.dispatcher = DefaultJobDispatcher( app )
  File /home/galaxy-dist/lib/galaxy/jobs/handler.py, line 704, in __init__
self.job_runners = self.app.job_config.get_job_runner_plugins( 
self.app.config.server_name )
  File /home/galaxy-dist/lib/galaxy/jobs/__init__.py, line 621, in 
get_job_runner_plugins
rval[id] = runner_class( self.app, runner[ 'workers' ], **runner.get( 
'kwds', {} ) )
  File /home/galaxy-dist/lib/galaxy/jobs/runners/drmaa.py, line 81, in 
__init__
self.ds.initialize()
  File /home/galaxy-dist/eggs/drmaa-0.7.6-py2.6.egg/drmaa/session.py, line 
257, in initialize
py_drmaa_init(contactString)
  File /home/galaxy-dist/eggs/drmaa-0.7.6-py2.6.egg/drmaa/wrappers.py, line 
73, in py_drmaa_init
return _lib.drmaa_init(contact, error_buffer, sizeof(error_buffer))
  File /home/galaxy-dist/eggs/drmaa-0.7.6-py2.6.egg/drmaa/errors.py, line 
151, in error_check
raise _ERRORS[code - 1](error_string)
AlreadyActiveSessionException: code 11: DRMAA session already exist.
[root@galaxy-project galaxy-dist]#


This is my “job_conf.xml”:

job_conf
plugins workers=4
plugin id=local type=runner 
load=galaxy.jobs.runners.local:LocalJobRunner/
plugin id=drmaa type=runner 
load=galaxy.jobs.runners.drmaa:DRMAAJobRunner/
plugin id=cli type=runner 
load=galaxy.jobs.runners.cli:ShellJobRunner /
plugin id=slurm type=runner 
load=galaxy.jobs.runners.slurm:SlurmJobRunner
param id=drmaa_library_path/usr/local/lib/libdrmaa.so/param
/plugin
/plugins
handlers
handler id=main/
/handlers
destinations default=drmaa_slurm
destination id=local runner=local/
destination id=multicore_local runner=local
  param id=local_slots4/param
  param id=embed_metadata_in_jobTrue/param
  job_metrics /
/destination
destination id=docker_local runner=local
  param id=docker_enabledtrue/param
/destination
destination id=drmaa_slurm runner=drmaa
param 
id=galaxy_external_runjob_scriptscripts/drmaa_external_runner.py/param
param 
id=galaxy_external_killjob_scriptscripts/drmaa_external_killer.py/param
param 
id=galaxy_external_chown_scriptscripts/external_chown_script.py/param
/destination
destination id=direct_slurm runner=slurm
param id=nativeSpecification--time=00:01:00/param
/destination
/destinations
resources default=default
  group id=default/group
  group id=memoryonlymemory/group
  group id=allprocessors,memory,time,project/group
/resources
tools
tool id=foo handler=trackster_handler
param id=sourcetrackster/param
/tool
tool id=bar destination=dynamic/
tool id=longbar destination=dynamic resources=all /
tool id=baz handler=special_handlers destination=bigmem/
/tools
limits
limit type=registered_user_concurrent_jobs2/limit
limit type=anonymous_user_concurrent_jobs1/limit
limit type=destination_user_concurrent_jobs id=local1/limit
limit type=destination_user_concurrent_jobs tag=mycluster2/limit
limit type=destination_user_concurrent_jobs tag=longjobs1/limit
limit type=destination_total_concurrent_jobs id=local16/limit
limit type=destination_total_concurrent_jobs 
tag=longjobs100/limit
limit type=walltime24:00:00/limit
limit type=output_size10GB/limit
/limits
/job_conf


Can you help me? I am newbie with “Galaxy Project” administration.




THANKS IN ADVANCE





Alfonso Pardo Diaz
System Administrator / Researcher
c/ Sola nº 1; 10200 Trujillo, ESPAÑA
Tel: +34 927 65 93 17 Fax: +34 927 32 32 37

[CETA-Ciemat logo]http://www.ceta-ciemat.es/





[galaxy-dev] Error report via API?

2014-09-25 Thread Evan Bollig
Is there an API endpoint for sending the error report for a failed
step in a history? I would like to run a workflow via API and email
the first error that occurs.

-Evan Bollig
Research Associate | Application Developer | User Support Consultant
Minnesota Supercomputing Institute
599 Walter Library
612 624 1447
e...@msi.umn.edu
boll0...@umn.edu
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[galaxy-dev] Simple standard for API use of a global user/key that all loaded tools can draw upon?

2014-09-25 Thread Dooley, Damion
Do any of you see the need to establish a way for tools to use a global 
(current site) API url/key combination?  From a security perspective I'm 
assuming this is basically ok since tools run under the galaxy user anyways 
(and can directly read and write files to their heart's content).  I understand 
that a particular user's API key could help limit what they have access to.  My 
tool however wants to do some prepatory form display and post-job work on 
behalf of the Galaxy system in addition to working with user-centric data; I 
presume others have similar use-case needs.

I think it would amount to having a standard GALAXY_TOOL_API_URL and 
GALAXY_TOOL_API_KEY managed in universe_wsgi.ini (left blank if not desired by 
sys admin).  The key would be generated by a galaxy admin using a real admin 
user id.  These would be available as python or shell environment variables 
when it came time to execute a tool's command interpreter=python etc. work. 
 

The wee other detail is that I'm using code file=versioned_data_form.py / 
in my tool xml because there is API information I have to retrieve to set up 
the form with.  So the url and key need to be accessible in that execution 
environment too?!!

Am I missing the boat; alternately has this already been talked about?!

Thanks for advice ...

Damion

Hsiao lab, BC Public Health Microbiology  Reference Laboratory, BC Centre for 
Disease Control
655 West 12th Avenue, Vancouver, British Columbia, V5Z 4R4 Canada
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Re: [galaxy-dev] Error report via API?

2014-09-25 Thread John Chilton
I don't believe this is currently available via the API - but we would
like to move everything to the API so I don't see why this wouldn't be
included (maybe with some rate limit-ting :)). I have created a Trello
card:

https://trello.com/c/W2PEpe8V

This could potentially be fairly straight forward to move from a web
controller to an API if you wanted to take this on and open a pull
request :).

Thanks for the suggestion,
-John

On Thu, Sep 25, 2014 at 3:14 PM, Evan Bollig boll0...@umn.edu wrote:
 Is there an API endpoint for sending the error report for a failed
 step in a history? I would like to run a workflow via API and email
 the first error that occurs.

 -Evan Bollig
 Research Associate | Application Developer | User Support Consultant
 Minnesota Supercomputing Institute
 599 Walter Library
 612 624 1447
 e...@msi.umn.edu
 boll0...@umn.edu
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Re: [galaxy-dev] sendmail from addr

2014-09-25 Thread John Chilton
Hey Evan,

The following diff *should* add a new config option in
universe_wsgi.ini called no_reply_email_from that can be used to set
from when sending these activation emails. If you are feeling brave,
try it out and let me know how it goes. I have not recreated this
problem (read I have not tested this at all) - but if this works for
you I would be happy to commit it to -central.

https://github.com/jmchilton/galaxy-central/commit/9f023a72a56bce5efb6b1cef1609f3993bf2291b
https://github.com/jmchilton/galaxy-central/commit/9f023a72a56bce5efb6b1cef1609f3993bf2291b.patch

-John

On Wed, Sep 24, 2014 at 5:56 PM, Evan Bollig boll0...@umn.edu wrote:
 On a local instance of Galaxy, I get this error when attempting to
 reset my password:

 Failed to reset password: (501, '5.1.7 Bad sender address syntax',
 'galaxy-no-reply@10.32.16.121')

 I am told my mail server requires the from address to either have a
 domain name, or the IP address to be in brackets (e.g.,
 [10.32.16.121]). Is there an option to configure the from address
 format, or override the address?

 If there are no configuration options, what files would I need to
 modify to add brackets? The domain name is not an option.

 Cheers,
 -Evan Bollig
 Research Associate | Application Developer | User Support Consultant
 Minnesota Supercomputing Institute
 599 Walter Library
 612 624 1447
 e...@msi.umn.edu
 boll0...@umn.edu
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