[galaxy-dev] GATK2 java version related issues

2014-10-10 Thread Iry Witham
Hi Team, I am sorry to be a pest, but I am under-the-gun to get this resolved. I have followed the instructions for installing GATK2 on my cloud VM. After doing that I discovered that the version of java running on the VM was v1.6.x and GATK2 required v1.7.0x. I removed version 6 and

Re: [galaxy-dev] GATK2 java version related issues

2014-10-10 Thread Daniel Blankenberg
First guess is that perhaps the system is still defaulting to using java 1.6 and not 1.7. What happens if you run “java -version” as the Galaxy system user? Perhaps try a reboot of the vm as well to make sure that the Galaxy app is running with java 1.7. Also, is this a custom vm? If it using

Re: [galaxy-dev] GATK2 java version related issues

2014-10-10 Thread Iry Witham
@lists.bx.psu.edu galaxy-dev@lists.bx.psu.edumailto:galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] GATK2 java version related issues First guess is that perhaps the system is still defaulting to using java 1.6 and not 1.7. What happens if you run “java -version” as the Galaxy system user? Perhaps try

[galaxy-dev] GATK2 reporting Invalid argument value '/mnt/galaxy/tools/gatk2/bin/' at position 8.

2014-10-09 Thread Iry Witham
Hi Team, I have installed GATK2 into a galaxy cloud instance to use the Unified Genotyper for our sequencing course. However, when I run the tool I get the following error message: Dataset 41: Unified Genotyper on data 8 and data 13 (VCF) Tool execution generated the following error message:

[galaxy-dev] gatk2 wrapper issue

2014-06-04 Thread Geert Vandeweyer
Hi, I installed the GATK2 wrapper from iuc on the lastest galaxy version. It fails to create the GATK2_PATH and GATK2_SITE_OPTIONS structures under the /tool_dependency_dir/environment_settings/ location, without a clear error (says 'never installed'). If I create the files manually, I can

Re: [galaxy-dev] gatk2 wrapper issue

2014-06-04 Thread Greg Von Kuster
Hello Geert, This issue was corrected yesterday in change sets https://bitbucket.org/galaxy/galaxy-central/commits/96fdb9168f28b2ffa463195d084c9278efb2465a and https://bitbucket.org/galaxy/galaxy-central/commits/58a057a02b896ae6d32571f7141f46ccb9006e54, which are currently available in both

Re: [galaxy-dev] GATK2 (from IUC) Missing reference_genome selection

2014-03-10 Thread Björn Grüning
Hi Geert, Hi, I'm using the GATK2 tools from IUC, taken from the main toolshed. After configuration, I'm able to run the tools as standalone jobs, and everything works. When I add the job to a workflow, The 'Using reference genome' selection is empty (see screenshot in attachment for

[galaxy-dev] GATK2 is unable to find reference genomes

2014-02-26 Thread Fenglou Mao
Dear All, I installed gatk2 from tool_shed, and see below for my gatk2_picard_index.loc Anyone can tell me what the mistake is? Old gatk can use this file to find all reference genomes, but gatk2 cannot. Fenglou gatk2_picard_index.loc Description: Binary data

Re: [galaxy-dev] GATK2 is unable to find reference genomes

2014-02-26 Thread Björn Grüning
Hi Fenglou, which file have you edited? Under tool-data or tool-data/toolshed.g2.bx.psu.edu/repos/iuc/gatk2/340633249b3d/gatk2_picard_index.loc ? Cheers, Bjoern Am 26.02.2014 22:37, schrieb Fenglou Mao: Dear All, I installed gatk2 from tool_shed, and see below for my

Re: [galaxy-dev] GATK2 is unable to find reference genomes

2014-02-26 Thread Fenglou Mao
Thanks, edited the wrong file. Fenglou On 26 February 2014 16:40, Björn Grüning bjoern.gruen...@gmail.com wrote: Hi Fenglou, which file have you edited? Under tool-data or tool-data/ toolshed.g2.bx.psu.edu/repos/iuc/gatk2/340633249b3d/gatk2_picard_index.loc? Cheers, Bjoern Am

Re: [galaxy-dev] GATK2 is unable to find reference genomes

2014-02-26 Thread Björn Grüning
No problem, your welcome. If you have any other feedback, please get in touch! Bjoern Am 26.02.2014 23:59, schrieb Fenglou Mao: Thanks, edited the wrong file. Fenglou On 26 February 2014 16:40, Björn Grüning bjoern.gruen...@gmail.com wrote: Hi Fenglou, which file have you edited? Under

[galaxy-dev] GATK2

2014-01-30 Thread Briand, Sheldon
Hi, I've installed the latest version (using GATK2.8-1) of the GATK2 wrapper here. I've run into the following error: # ERROR MESSAGE: The fasta file you specified (/tmp/tmp-gatk-V7IrBw) does not exist. I see via much googling that this error is due to a user input problem. I've tried

Re: [galaxy-dev] GATK2

2014-01-30 Thread Björn Grüning
Hi, do you have access to the generated commandline from Galaxy and can paste it her? I’ve installed the latest version (using GATK2.8-1) of the GATK2 wrapper here. I’ve run into the following error: # ERROR MESSAGE: The fasta file you specified (/tmp/tmp-gatk-V7IrBw) does not

Re: [galaxy-dev] GATK2

2014-01-30 Thread Briand, Sheldon
Message- From: Björn Grüning [mailto:bjoern.gruen...@gmail.com] Sent: Thursday, January 30, 2014 12:26 PM To: Briand, Sheldon Cc: galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] GATK2 Hi, do you have access to the generated commandline from Galaxy and can paste it her? I’ve installed

Re: [galaxy-dev] GATK2

2014-01-30 Thread Björn Grüning
/packages/galaxy/galaxy-dist/database/pbs/101.ec Thanks, -Sheldon -Original Message- From: Björn Grüning [mailto:bjoern.gruen...@gmail.com] Sent: Thursday, January 30, 2014 12:26 PM To: Briand, Sheldon Cc: galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] GATK2 Hi, do

Re: [galaxy-dev] GATK2

2014-01-30 Thread bjoern.gruen...@googlemail.com
-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] GATK2 Hi Sheldon, The loc file is tabular. I put the 2bit file in the directory and had no success. Here is the rather lengthy commandline: #!/bin/sh export GALAXY_SLOTS_CONFIGURED=1 if [ -n $SLURM_JOB_NUM_NODES

[galaxy-dev] GATK2 Galaxy wrapper available, testing and help needed

2014-01-18 Thread Björn Grüning
Hi all, over the last month we developed a new version of GATK2 wrappers and are now seeking for testers, users and overall feedback. We are currently targeting GATK 2.8. One of the big issues with GATK2 is the new licence. Because of that we can't install GATK2 with the wrappers, but we hope we