Re: [galaxy-dev] Tophat non Sanger input

2011-09-08 Thread Stephen Taylor
On 07/09/2011 20:22, Edward Kirton wrote: seems unnecessary since illumina switched over to fastqsanger now. http://www.illumina.com/truseq/quality_101/quality_scores.ilmn Eventually...unfortunately we still get a lot of fastqillumina :-( Steve

[galaxy-dev] upload files from file system

2011-09-08 Thread shashi shekhar
Hi, In my local instance of Galaxy ,I want to make change in "upload files from file system" option which is used admin. in which file i have to make changes ? i want to attach some more features in that. Regards shashi ___ Please keep

Re: [galaxy-dev] Automatically removing items from history

2011-09-08 Thread Dannon Baker
I haven't had a chance to do anything on this yet, but I'll see if I can work something out in the near future. -Dannon On Sep 7, 2011, at 9:34 PM, Glen Beane wrote: > > On Sep 7, 2011, at 8:10 PM, Edward Kirton wrote: > >> i'm resurrecting this thread to see if there's any more support for t

[galaxy-dev] trannsfer files from remote system to galaxy

2011-09-08 Thread shashi shekhar
Hi, In my local instance of Galaxy ,I want to add one option in which i can get files from remote system to galaxy in data library . except url is there any option to get remote files from galaxy ? Regards ___ Please keep all replies

Re: [galaxy-dev] Tophat non Sanger input

2011-09-08 Thread Hans-Rudolf Hotz
On 09/08/2011 09:47 AM, Stephen Taylor wrote: On 07/09/2011 20:22, Edward Kirton wrote: seems unnecessary since illumina switched over to fastqsanger now. http://www.illumina.com/truseq/quality_101/quality_scores.ilmn Eventually...unfortunately we still get a lot of fastqillumina :-( I m

Re: [galaxy-dev] upload files from file system

2011-09-08 Thread Greg Von Kuster
Shashi, Probably the best place to start is the upload_library_dataset method in ~/lib/galaxy/web/controllers/library_common.py. good luck, Greg Von Kuster On Sep 8, 2011, at 6:42 AM, shashi shekhar wrote: > Hi, > > In my local instance of Galaxy ,I want to make change in "upload > files f

Re: [galaxy-dev] Tophat non Sanger input

2011-09-08 Thread Stephen Taylor
On 08/09/2011 14:17, Hans-Rudolf Hotz wrote: On 09/08/2011 09:47 AM, Stephen Taylor wrote: On 07/09/2011 20:22, Edward Kirton wrote: seems unnecessary since illumina switched over to fastqsanger now. http://www.illumina.com/truseq/quality_101/quality_scores.ilmn Eventually...unfortunately

Re: [galaxy-dev] Tophat non Sanger input

2011-09-08 Thread Anton Nekrutenko
Dear Stephen (and others): The sole reason for requiring fastq-sanger input to all of our wrappers was to force the users to run their data through the groomer. It is slow, but it checks data consistency in a way that is more robust than just checking 'four lines per fastq block' and prevents a

Re: [galaxy-dev] Tophat non Sanger input

2011-09-08 Thread Whyte, Jeffrey
Anton, If a user is running a multi-core machine, a simple method to speed up FASTQ -Groomer is to first split the original FASTQ file (e.g. into 10 smaller files if you've got a 12-core machine), run FASTQ-Groomer on each file concurrently, then join the 10 files back together. This allows fo

Re: [galaxy-dev] Installation error

2011-09-08 Thread IT Support
My co-worker solved it. You were both right about the Python version. This was a very silly mistake. export PATH=/opt/galaxy-python/python:$PATH Should have been export PATH=/opt/galaxy-python:$PATH So I basically put the binary (and not the _path_ to the binary) in the $PATH variable. This l

[galaxy-dev] Server down?

2011-09-08 Thread Crystal Goh
Hi, I am user of Galaxy Test. I wonder is the Galaxy Test server down these 2 days? As the Tophat and Cufflinks job running take me a lot of time to run compared to previous? Thanks. Best regards, Crystal _

Re: [galaxy-dev] cuffcompare wrapper

2011-09-08 Thread Chorny, Ilya
It's not an issue in the tmp dir but in the job_working_directory. I run most of those other tools with no problems. I don't think we should make it a requirement across the board and I think we can come up with alternative ways to clean up the job_working_directory. I am hoping that you could a

Re: [galaxy-dev] macs in galaxy

2011-09-08 Thread Chorny, Ilya
Is there a python script associated with the macs.xml file? From: galaxy-dev-boun...@lists.bx.psu.edu [mailto:galaxy-dev-boun...@lists.bx.psu.edu] On Behalf Of Kanwei Li Sent: Wednesday, August 24, 2011 5:41 PM To: KOH Jia Yu Jayce Cc: galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] macs in

Re: [galaxy-dev] macs in galaxy

2011-09-08 Thread Kanwei Li
I don't believe so, it looks like they generate a long config script (as you can see in the xml file) and executes that. K On Thu, Sep 8, 2011 at 1:51 PM, Chorny, Ilya wrote: > Is there a python script associated with the macs.xml file? > > ** ** > > *From:* galaxy-dev-boun...@lists.bx.psu

Re: [galaxy-dev] disk space and file formats

2011-09-08 Thread Edward Kirton
copied from another thread: On Thu, Sep 8, 2011 at 7:30 AM, Anton Nekrutenko wrote: > What we are thinking of lately is switching to unaligned BAM for > everyting. One of the benefits here is the ability to add readgroups from > day 1 simplifying multisample analyses down the road. > this seems

Re: [galaxy-dev] disk space and file formats

2011-09-08 Thread Fields, Christopher J
The use of (unaligned) BAM for readgroups seems like a good idea. At the very least it prevents inconsistently hacking this information into the FASTQ descriptor (a common problem with any simple format). chris On Sep 8, 2011, at 1:35 PM, Edward Kirton wrote: > copied from another thread: >

Re: [galaxy-dev] galaxy-dev Digest, Vol 63, Issue 8

2011-09-08 Thread Andrew Warren
I would also like to voice my support for this feature. I wrote a wrapper for bowtie that converts the SAM output to BAM after bowtie is finished just to avoid the hassle of letting galaxy "know" that the SAM file existed (didn't want to run Tophat). After thinking about how I would go about deleti

Re: [galaxy-dev] cuffcompare wrapper

2011-09-08 Thread Jeremy Goecks
I'm confused. Why would the symlink cause problems for Cuffcompare but not for other tools that use symlinks (including Cufflinks and Cuffdiff)? J. On Sep 8, 2011, at 1:43 PM, Chorny, Ilya wrote: > It’s not an issue in the tmp dir but in the job_working_directory. I run most > of those other t