u]
> Sent: Wednesday, May 18, 2011 11:45 AM
> To: Robert Curtis Hendrickson
> Cc: galaxy-user
> Subject: Re: [galaxy-user] UCSC->EMBOSS/fuzznuc->UCSC workflow?
>
> Hello Curtis,
>
> The BED extraction data can be resolved in Galaxy. Pull out the whole
> gene and t
fuzznuc
Any advice would be appreciated.
Curtis
-Original Message-
From: Jennifer Jackson [mailto:j...@bx.psu.edu]
Sent: Wednesday, May 18, 2011 11:45 AM
To: Robert Curtis Hendrickson
Cc: galaxy-user
Subject: Re: [galaxy-user] UCSC->EMBOSS/fuzznuc->UCSC workflow?
Hello Curtis,
The BED extract
ndrickson
> Cc: galaxy-user
> Subject: Re: [galaxy-user] UCSC->EMBOSS/fuzznuc->UCSC workflow?
>
> Hello Curtis,
>
> The BED extraction data can be resolved in Galaxy. Pull out the whole
> gene and then modify the coordinates in Galaxy to be 10k upstream.
>
> To be c
, they don’t carry any sequence.
Have I mis-understood your directions?
Regards,
Curtis
-Original Message-
From: Jennifer Jackson [mailto:j...@bx.psu.edu]
Sent: Tuesday, May 17, 2011 11:23 AM
To: Robert Curtis Hendrickson
Cc: 'galaxy-user@lists.bx.psu.edu'
Subject: Re: [galaxy-user] U
50 PM
To: Robert Curtis Hendrickson
Cc: 'galaxy-user@lists.bx.psu.edu'
Subject: Re: [galaxy-user] UCSC->EMBOSS/fuzznuc->UCSC workflow?
Hello Curtis,
The coordinates of your match are with respect to the fasta sequence,
not with respect to the reference genome. Only data mapped to the
From: Jennifer Jackson [mailto:j...@bx.psu.edu]
Sent: Monday, May 16, 2011 6:50 PM
To: Robert Curtis Hendrickson
Cc: 'galaxy-user@lists.bx.psu.edu'
Subject: Re: [galaxy-user] UCSC->EMBOSS/fuzznuc->UCSC workflow?
Hello Curtis,
The coordinates of your match are with respe
Hello Curtis,
The coordinates of your match are with respect to the fasta sequence,
not with respect to the reference genome. Only data mapped to the
reference genome can be viewed in the UCSC Browser
You will need to calculate from the position of the match in the fasta
sequence back throug
Folks,
I wanted to scan the 2kb upstream of a list of human gene isoforms for TFBS
using fuzznuc. I was able to
"Get Data"> "UCSC Main" > "As sequence" and get my sequences
"EMBOSS" > fuzznuc ran fine, and output the hits
HOWEVER, fuzznuc lost the genomic position information that UCSC has put
8 matches
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