Re: [galaxy-user] BAM files

2011-04-20 Thread Peter Cock
On Wed, Apr 20, 2011 at 4:00 AM, Edward Dudley egd...@psu.edu wrote: Hi - I have a set of 454 reads that have been trimmed and converted to BAM format using Galaxy, and I can visualize the alignment with E. coli genomes using the UCSC browser.  Problem is, I'd like to display the alignment in

Re: [galaxy-user] BAM files

2011-04-20 Thread Ann Loraine
Are you downloading the ³BAI² index file, as well? That might be the problem. Best, Ann On 4/19/11 11:00 PM, Edward Dudley egd...@psu.edu wrote: Hi - I have a set of 454 reads that have been trimmed and converted to BAM format using Galaxy, and I can visualize the alignment with E. coli

[galaxy-user] Reference genome question, arabidopsis

2011-04-20 Thread Rick Westerman
Hello all. I am re-exploring Galaxy after a year hiatus. While I searched the archives to no avail it is possible that my question/complaint was already answered. If so, please ignore. I find the placement of Mouse-ear Cress (arabidopsis) in the 'select a reference genome' list to be

Re: [galaxy-user] ce6/WS190 bowtie index

2011-04-20 Thread Jennifer Jackson
Hello - Few more links that may be helpful: http://bitbucket.org/galaxy/galaxy-central/wiki/DataTables https://bitbucket.org/galaxy/galaxy-central/wiki/ToolDependencies Thanks! Jen On 4/20/11 1:49 PM, Jennifer Jackson wrote: Hello Giovanni, Galaxy does not (yet) support genome downloads,

Re: [galaxy-user] get wig file after tophat

2011-04-20 Thread Ying Zhang
Dear Ann and Jeremy: We have this discussion long time ago, and I am sorry that I brought it up here again. I am just thinking that as Ann said, can we add this tool which convert bam into wig file into galaxy? Or make a workflow to generate a wig file from a bam file generate by tophat? In

Re: [galaxy-user] Normalization and plotting of RPKM/FPKM after cufflink

2011-04-20 Thread Jeremy Goecks
Vasu, Here are the steps to create this visualization; this is relatively new functionality, and you'll want to use our test server ( http://test.g2.bx.psu.edu/ ) for now. (1) Create a new visualization from the main menu: Visualization -- New Track Browser and choose your genome build. (2)

Re: [galaxy-user] get wig file after tophat

2011-04-20 Thread Jeremy Goecks
Hi Ying, You're in luck because I've been working with genome browsers lately, so I think I can help you address your problem. What you're looking for is a visualization of a coverage histogram for the BAM reads produced by Tophat, yes? It turns out that some genome browsers provide this

Re: [galaxy-user] get wig file after tophat

2011-04-20 Thread Jim Robinson
I can answer IGV questions, sadly I'm still coming up to speed on Galaxy. I've lost track of the original question, but IGV computes a coverage histogram on the fly, a bit like the Galaxy Track Browser, but you have to be zoomed in. However, you can also precompute a coverage

[galaxy-user] How to receive digest only?

2011-04-20 Thread Yi Huang
Hi everyone, I'd like to know if i can receive digested mail only. Thanks Sincerely, John ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep