[galaxy-user] change queue with sge

2011-05-03 Thread Sylvain Thomas

Hi

I currently use a galaxy server with cluster. This cluster uses SGE.
I'd like to specify a queue other than the default.
I have tried many combinaisons with drmaa:/// without success. The queue 
used is always the default one.


Does anyone has solved this problem?

Thanks,

Sylvain

--
Sylvain Thomas
tel : +33 (0)5 61 28 54 27

INRA - Unité de BIA
Génopôle - plateforme Bio-informatique
Chemin de Borde-Rouge - AUZEVILLE
BP 52627 - 31326 CASTANET-TOLOSAN CEDEX

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Re: [galaxy-user] quality stat.

2011-05-03 Thread Peter Cock
On Tue, May 3, 2011 at 10:00 AM, Robin Mjelle robinmje...@gmail.com wrote:
 Dear User,

 I am trying to use the tool Compute quality statistics in galaxy on
 Ilumina single reads. The file is 2.3 Gb, fastq format. I have performed
 Quality format converter on the data set and the format is now qualillumina.
 Despite of this, galaxy don't recognize any dataset in the workflow to use
 as input into quality statistics.
 Any idea why my dataset is not accepted as input?

 Best,

 Robin

Looking at the XML wrapper, it expects fastqsanger ONLY. See
fastx_toolkit/fastx_quality_statistics.xml

It could in theory take fastqillumina or fastqsolexa as well, I thought
there was an open bug report on this issue but I can't find it right
now. Certainly fastx_clipper.xml was updated to use the -Q 33
switch only for Sanger quality scores, and from a quick check all
the other FASTX tools have this fix except fastx_quality_statistics.xml

Peter
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[galaxy-user] SNPs, Indels and so on in virus genomes

2011-05-03 Thread David Matthews
Hi,

I recently sent out an email asking if anyone knew much about analysis of SNPs 
etc and how to visualise them. I got some very useful answers and planned to 
return to the problem when I got a better chance to work on this in some depth. 
I now have the time but, like an idiot, I've accidentally deleted those email 
replies! So can I please ask again, does anyone have experience of SNP analysis 
and, especially, visualisation that can hold my hand whilst I work this out 
(apologies to the ones who replied last time but can you get in touch again !)?


Best Wishes,
David.

__
Dr David A. Matthews

Senior Lecturer in Virology
Room E49
Department of Cellular and Molecular Medicine,
School of Medical Sciences
University Walk,
University of Bristol
Bristol.
BS8 1TD
U.K.

Tel. +44 117 3312058
Fax. +44 117 3312091

d.a.matth...@bristol.ac.uk




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Re: [galaxy-user] SNPs, Indels and so on in virus genomes

2011-05-03 Thread Jennifer Jackson

Hi David,

For NGS data, the tools under NGS: Indel Analysis can build simple BED 
files of indels from SAM files that can be viewed in Galaxy 
(Visualization tab - i.e. Trackster). For viral genomes, you will not be 
able to use UCSC. The Galaxy 101 tutorial includes some basic SNP 
analysis and visualization tasks that may help you to orient:

http://main.g2.bx.psu.edu/u/aun1/p/galaxy101

You may get some more replies, but to pull up old responses on the 
galaxy-user and galaxy-dev mailing list, the archives are here:


http://lists.bx.psu.edu/pipermail/galaxy-user/
http://lists.bx.psu.edu/pipermail/galaxy-dev/

To search, use google and enter:

site:http://lists.bx.psu.edu/pipermail

plus enter whatever keywords would identify your questions (perhaps your 
name?)


Other list members are encouraged to reply to David's inquiry with more 
help again,


Best!

Jen
Galaxy team


On 5/3/11 10:03 AM, David Matthews wrote:

Hi,

I recently sent out an email asking if anyone knew much about analysis
of SNPs etc and how to visualise them. I got some very useful answers
and planned to return to the problem when I got a better chance to work
on this in some depth. I now have the time but, like an idiot, I've
accidentally deleted those email replies! So can I please ask again,
does anyone have experience of SNP analysis and, especially,
visualisation that can hold my hand whilst I work this out (apologies to
the ones who replied last time but can you get in touch again !)?


Best Wishes,
David.

__
Dr David A. Matthews

Senior Lecturer in Virology
Room E49
Department of Cellular and Molecular Medicine,
School of Medical Sciences
University Walk,
University of Bristol
Bristol.
BS8 1TD
U.K.

Tel. +44 117 3312058
Fax. +44 117 3312091

d.a.matth...@bristol.ac.uk mailto:d.a.matth...@bristol.ac.uk






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--
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http://usegalaxy.org
http://galaxyproject.org
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Re: [galaxy-user] quality stat.

2011-05-03 Thread Jennifer Jackson

Hi Robin,

Run the FASTQ Groomer on your datafile first, then use the result as 
input to the Compute quality statistics tool. (Skip using Quality format 
converter).


Hopefully this helps,

Jen
Galaxy team

On 5/3/11 2:00 AM, Robin Mjelle wrote:

Dear User,

I am trying to use the tool Compute quality statistics
http://main.g2.bx.psu.edu/tool_runner?tool_id=cshl_fastx_quality_statistics
in galaxy on Ilumina single reads. The file is 2.3 Gb, fastq format. I
have performed Quality format converter on the data set and the format
is now qualillumina. Despite of this, galaxy don't recognize any dataset
in the workflow to use as input into quality statistics.
Any idea why my dataset is not accepted as input?

Best,

Robin



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Galaxy analysis and other features on the public server
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--
Jennifer Jackson
http://usegalaxy.org
http://galaxyproject.org
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