Re: [galaxy-user] FASTQ type change

2011-02-17 Thread Daniel Blankenberg
Hi Stephen and Peter, This change is definitely possible, and because the SAM format specifies that its quality scores are phred scaled and ASCII offset of 33 (regardless of provided input) it shouldn't cause complications downstream. We'll add this to our todo list. I created a ticket: https:

[galaxy-user] GeneTrack Server Error

2011-02-17 Thread Felix Hammer
Hi, I am trying to view Converted Interval Data in GeneTrack, but all I get is "Server Error An error occurred. See the error logs for more information. (Turn debug on to display exception reports here)" thx, Felix ___ The Galaxy User list should be us

[galaxy-user] Importing GTF into Galaxy

2011-02-17 Thread Martin, David A.
Hi, I have been trying to get reference data from the ucsc browser into Galaxy, but when I try to get the rat genome in gtf format, I only get files halfway through chomosome 10. This happens with both of the available builds for the rat. I am guessing this is a problem with the UCSC files a

Re: [galaxy-user] FastX Clipper on FastQ data

2011-02-17 Thread Daniel Blankenberg
Hi Mike, I was able to select fastqsanger files from one of my histories at our public server. If you are using the public server, can you share your history with me and I will check if there is a reason you are unable to select these datasets. If you are trying this on your local instance, ca

Re: [galaxy-user] Upload via SCP?

2011-02-17 Thread Nate Coraor
Felix Hammer wrote: > Hi, > is it possible to use SCP to upload files instead of FTP? Hi Felix, At this time, no. --nate > thxbye, > Felix > ___ > The Galaxy User list should be used for the discussion > of Galaxy analysis and other features on the pu

Re: [galaxy-user] FastX Clipper on FastQ data

2011-02-17 Thread Michael Walter
Dear Peter, Thanks for the quick reply. But yes, the groomer output was marked as fastqsanger. I changed to generic fastq and back and it didn't work either way. I only get the fasta files from my history as possible input files. However, using the local installation via command line seemed to

Re: [galaxy-user] Filename extension in new tool

2011-02-17 Thread Sean Davis
On Thu, Feb 17, 2011 at 5:48 AM, Peter Cock wrote: > On Thu, Feb 17, 2011 at 3:00 AM, Sean Davis wrote: > > I have a tool that takes a pdb file as input. The authors of the > *compiled* > > code require that the suffix be either ".pdb" or ".ent". When I upload a > > .pdb file, the filename that

[galaxy-user] Upload via SCP?

2011-02-17 Thread Felix Hammer
Hi, is it possible to use SCP to upload files instead of FTP? thxbye, Felix ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. For discussion of local Galaxy instances a

Re: [galaxy-user] FASTQ type change

2011-02-17 Thread Peter Cock
On Thu, Feb 10, 2011 at 6:42 PM, Peter wrote: > On Thu, Feb 10, 2011 at 6:34 PM, Stephen Taylor wrote: >> >> Sounds like this should be added into the main release. It would save a lot >> of time/disk space instead of using Groomer. >> > > I agree. Maybe Dan can take care of it - I don't have bowti

Re: [galaxy-user] Filename extension in new tool

2011-02-17 Thread Peter Cock
On Thu, Feb 17, 2011 at 3:00 AM, Sean Davis wrote: > I have a tool that takes a pdb file as input.  The authors of the *compiled* > code require that the suffix be either ".pdb" or ".ent".  When I upload a > .pdb file, the filename that gets fed to the tool now ends in .dat.  What is > the best wa

Re: [galaxy-user] FastX Clipper on FastQ data

2011-02-17 Thread Peter Cock
On Thu, Feb 17, 2011 at 10:06 AM, Michael Walter wrote: > > Hi List, > > I have a couple of miRNA-Seq files (Illumina GAIIx, 29nt read length). These > reads contain differing amounts of 3' adapter sequences and therefore map > really badly to the human genome (with eland v2). Therefore, I'd like

[galaxy-user] FastX Clipper on FastQ data

2011-02-17 Thread Michael Walter
Hi List, I have a couple of miRNA-Seq files (Illumina GAIIx, 29nt read length). These reads contain differing amounts of 3' adapter sequences and therefore map really badly to the human genome (with eland v2). Therefore, I'd like to use the FastX clipper tool, which states that "This tool clip