Re: [galaxy-user] zero vs 1 based

2011-09-12 Thread Jennifer Jackson
Hello Richard, The coordinates have a zero-based start. Add +1 to the start, do nothing to the end, and the bases included will match up with any visualization tool where the first base is labeled 1. The data: chr10 1056309 1056310 G C + start = 1056309 + 1 = 1056310 end = 1056310 SNP is a

[galaxy-user] Installing Galaxy on my computer

2011-09-12 Thread Lilach F
Hi, I am a new user of Galaxy. I want to analyze whole human exome data with Galaxy and cannot upload the files (10 Gb each) to the server. Should I install Galaxy on a PC, and work in Unix environment, or there is a more comfortable way? If I should install it - what should be the computer

Re: [galaxy-user] Need help with local install of Galaxy

2011-09-12 Thread Jennifer Jackson
Hello Aarti, On 9/12/11 1:26 AM, Aarti Desai wrote: Hi Jennifer, I have previously used the web version of Galaxy for doing some RNASeq analysis and I loved it! I now have some more RNASeq and ChIPSeq data I want to analyze using Galaxy, but the file sizes are more than 2GB and hence am

Re: [galaxy-user] Need help with local install of Galaxy

2011-09-12 Thread Lilach F
Hi Aarti, I also tried to upload big files (10 Gb) to the Galaxy through FTP, according to the instructions, but my internet is too slow and it didn't succeed (I left the computer open for days, but it always crashed in the middle). Therefore, I want to install Galaxy on a single computer, and

[galaxy-user] trouble Clipping barcodes on data

2011-09-12 Thread KAKOLIE GOSWAMI
Dear Galaxy Users,I have a data set, for which I'm trying to Clip adapter sequences. After splitting the generated data based on different Barcode sequences, I clip the adapter sequences off. This method worked for all the other data sets that I worked with, but seems to act up with one particular

Re: [galaxy-user] Installing Galaxy on my computer

2011-09-12 Thread Peter Cock
On Mon, Sep 12, 2011 at 5:20 PM, Lilach F lilac...@gmail.com wrote: Hi Jennifer, I already tried 2 weeks ago to upload my whole exome data by FTP, according to the instructions you sent below. The files are too big, it should take days for each file, and it always stops in the middle after 12 

Re: [galaxy-user] Installing Galaxy on my computer

2011-09-12 Thread Lilach F
Thank you very much for the big help! Lilach 2011/9/12 Peter Cock p.j.a.c...@googlemail.com On Mon, Sep 12, 2011 at 5:20 PM, Lilach F lilac...@gmail.com wrote: Hi Jennifer, I already tried 2 weeks ago to upload my whole exome data by FTP, according to the instructions you sent below.

Re: [galaxy-user] Installing Galaxy on my computer

2011-09-12 Thread Lilach F
Hi Peter, Thank you very much for the help! Another question: What type of instance I should select in order to analyze 2 whole exomes, 20 Gb each (total: 40 Gb)? Thanks, Lilach 2011/9/12 Lilach F lilac...@gmail.com Thank you very much for the big help! Lilach 2011/9/12 Peter Cock

Re: [galaxy-user] Upload of most recent genome data for Apis mellifera onto Galaxy and/or NCSC web sites?

2011-09-12 Thread Anton Nekrutenko
Diana: It is best to direct such requests to galaxy-u...@bx.psu.edu mailing list, which I am doing. Adding this genome should be possible, but will take us some time. Thanks, anton Anton Nekrutenko http://galaxyproject.org On Sep 12, 2011, at 1:23 PM, Diana Cox-Foster wrote: Hi,

[galaxy-user] How to use GATK Unified Genotyper with A list of genomic intervals over which to operate option

2011-09-12 Thread Carlos Borroto
Hi, I would like to run Unified Genotyper on a region of a BAM file, I see the advance option A list of genomic intervals over which to operate exist and seems to be what I need. The problem is I only get a drop-down menu with the single option Selection is optional, which I don't understand.