Re: [galaxy-user] Drosophila yakuba reference genome

2012-03-21 Thread Jennifer Jackson
Hello, The quickest way to use a genome that is not already indexed is to load it into you history and select it as a custom genome from the TopHat tool form with the option: "Will you select a reference genome from your history or use a built-in index?:" -> "Use one from the history" Inst

[galaxy-user] Chip-seq exercise

2012-03-21 Thread Joshua Udall
Does anyone know what happened to the chip-seq exercise by James? It was part of a collection here: http://main.g2.bx.psu.edu/u/james/p/exercises and it was linked here: http://main.g2.bx.psu.edu/u/james/p/exercise-chip-seq But is it 'Not Found'. It was a very useful exercise. Will it be back

Re: [galaxy-user] Bowtie Seed length (-l) and non-unique reads mode (m=-1)

2012-03-21 Thread Jennifer Jackson
Hello Eric, I apologize, but I made a mistake, please see below On 3/21/12 5:42 AM, Jennifer Jackson wrote: Hi Eric, On 3/19/12 4:13 PM, Eric Guo wrote: Hi there, I have two questions regarding alignment using Bowtie: 1. Is there a way to set the Seed Length (-l) to the full length of each

[galaxy-user] Drosophila yakuba reference genome

2012-03-21 Thread Evgeny Brud
Hi, Could galaxy include the Drosophila yakuba reference genome for use with Tophat? It's not listed. ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Plea

[galaxy-user] (no subject)

2012-03-21 Thread Moein Farshchian
hi to all Would you please someone help me to remove my email from galaxy users list? Thanks. Moein ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Ple

[galaxy-user] "Job is waiting to run"

2012-03-21 Thread Shisheng Li
Dear Sir/Madam, I am a registered user of the public Galaxy Server (main). Any job I submitted today is labeled as "Job is waiting to run" forever. The total volume of my files is 249 mb, which is far below the quota limit of 250 gb, and I am trying to run less than 8 jobs. Could you please let me

Re: [galaxy-user] Deploying a tool

2012-03-21 Thread Greg Von Kuster
Hello Mark, You can automatically install tools from any accessible Galaxy tool shed to your local Galaxy instance. The Galaxy tool shed wiki provides all of the information you'll need to do this. I recommend reading the entire wiki if you haven't already done so. http://wiki.g2.bx.psu.edu/

[galaxy-user] Deploying a tool

2012-03-21 Thread Mark Maienschein-Cline
Hi, This is a simple question, but I couldn't find an explicit explanation online. How do I deploy a tool from the Galaxy toolshed to my local version of Galaxy? Do I have to download the package and move the files into the tools/ directory, and update the tool list, or is there an automatic wa

Re: [galaxy-user] How to add alignment to the MAF

2012-03-21 Thread Jennifer Jackson
Hello Lyni, Are you still having problems with your analysis? If so, then I can point you to a few tools that can help. - "FASTA manipulation" -> "Tabular-to-FASTA" and "FASTA-to-Tabular" - "Text Manipulation" -> "Concatenate datasets" - "Filter and Sort" -> "Sort" I am not sure how many RN

Re: [galaxy-user] Bowtie Seed length (-l) and non-unique reads mode (m=-1)

2012-03-21 Thread Jennifer Jackson
Hi Eric, On 3/19/12 4:13 PM, Eric Guo wrote: Hi there, I have two questions regarding alignment using Bowtie: 1. Is there a way to set the Seed Length (-l) to the full length of each read instead of using a single Seed Length for all reads? The seed length is a single input value for all seque

Re: [galaxy-user] Trouble with BLAST+ and .loc file

2012-03-21 Thread Makis Ladoukakis
Dear Peter, Thank you for your reply. You are right I do have all 6 parts of the nr database on the server plus the .pal file in the same directory. The .loc file still is: database [build data] path nr_01_Mar_2012 nr 15 Mar 2012 /path/on/the/server/nr_directory/nr but the

Re: [galaxy-user] Trouble with BLAST+ and .loc file

2012-03-21 Thread Peter Cock
On Wed, Mar 21, 2012 at 10:21 AM, Peter Cock wrote: > 2012/3/21 Makis Ladoukakis : >> Dear Galaxy users, >> >> I have been trying to upload a blastable database in my local instance of >> galaxy. I have used the nr database and generated all the nhr, nin, nsq, and >> nal files. I have also edited

Re: [galaxy-user] Trouble with BLAST+ and .loc file

2012-03-21 Thread Björn Grüning
Hi Makis, here is my working nr entry: /media/data/ncbi/blast/db/unpacked_nt/ntncbi_nt/media/data/databases/ncbi/blast/db/unpacked_nt/nt i was not able to see if your tabs are correct. So maybe its just a wrongly formatted loc file. Cheers, Bjoern P.S. You can download preformatted blast-datab

Re: [galaxy-user] Trouble with BLAST+ and .loc file

2012-03-21 Thread Peter Cock
2012/3/21 Makis Ladoukakis : > Dear Galaxy users, > > I have been trying to upload a blastable database in my local instance of > galaxy. I have used the nr database and generated all the nhr, nin, nsq, and > nal files. I have also edited the blastdb.loc file in the > galaxy-dist/tool-data/ directo

[galaxy-user] Trouble with BLAST+ and .loc file

2012-03-21 Thread Makis Ladoukakis
Dear Galaxy users, I have been trying to upload a blastable database in my local instance of galaxy. I have used the nr database and generated all the nhr, nin, nsq, and nal files. I have also edited the blastdb.loc file in the galaxy-dist/tool-data/ directory and it looks like this: datab