[galaxy-user] metagenomics question

2012-07-11 Thread Jennifer Jackson
Hello, The publication and supplemental material for the metagenomics data and tools available in Galaxy described in: Windshield splatter analysis with the Galaxy metagenomic pipeline is available on the main public Galaxy instance at: Shared Data - Shared Published Pages - Windshield

Re: [galaxy-user] Initial QC and grooming for Illumina HiSeq2000 paired end RNAseq data

2012-07-11 Thread Lindsey Kelly
Good Morning, I confirmed with Illumina that my reads are in Sanger FASTQ format and used edit attributes to change them to fastqsanger. Then I joined the forward and reverse and ran the FastQ Joiner and the FastQC on the joined data file. I'm getting the following error: ## odpath=None: No

Re: [galaxy-user] Initial QC and grooming for Illumina HiSeq2000 paired end RNAseq data

2012-07-11 Thread Jennifer Jackson
Hello Lindsey, Would you please send in a bug report from the FastQC error dataset that resulted from the run where you included running the Groomer tool first? This will help us to track down the problem. Please be sure to leave all datasets in your history for this run undeleted and in

[galaxy-user] Gene BED to codon BED not working properly

2012-07-11 Thread Antony Jose
Hi, When using the gene BED to codon BED tool, I noticed that it is not accurately reporting the codons that make up a gene. For example, some of the codon are missing (particularly ones that span exon-exon junctions. Also, when changing reading frame from one exon to the next, the codons are not

Re: [galaxy-user] Gene BED to codon BED not working properly

2012-07-11 Thread Jennifer Jackson
Hi Anthony, There are no known problems with the gene/codon BED tool, but if you have an example of a problem, I will take a look. Some items to double check first: 1 - Input is a BED 12 file 2 - Keep in mind that coordinates are 0-based, half-open start, fully closed end and always