[galaxy-user] Problem with executable file in FastQC

2013-12-09 Thread Jorge Braun
Hi mates!! I have a problem with Galaxy... FastQC doesn't run because the file FastQC.py cann't find executable file FastQC.xml. Almost, that file is in the same directory called rgenetics, (seeing Linux terminal) . someone can help me? thanks and have a nice day :)

Re: [galaxy-user] Problem with executable file in FastQC

2013-12-09 Thread Ross
Hi, Jorge, Galaxy source includes the Galaxy interfaces but not the third party executables for tools like fastqc or bwa. They can be automagically installed if you install the tool from a tool shed but at a guess, you are working on your desktop with the fastqc tool in a recent clone of galaxy?

Re: [galaxy-user] Problem with executable file in FastQC

2013-12-09 Thread Jorge Braun
thanks for the information Ross but I'm lost with the guide for the fastqc tool. I don't understand how to resolve missing tool dependecies Date: Mon, 9 Dec 2013 22:30:58 +1100 Subject: Re: [galaxy-user] Problem with executable file in FastQC From: ross.laza...@gmail.com To:

Re: [galaxy-user] Problem with executable file in FastQC

2013-12-09 Thread Jennifer Jackson
HI Jorge, I am going to assume that you are working with a local instance, but please correct us if that is wrong. Going forward, you will want to ask local install questions at: galaxy-...@bx.psu.edu, as that is where the development community is most active. Lists:

Re: [galaxy-user] How to filter the sequences containing not[ATCG] character?

2013-12-09 Thread Jennifer Jackson
Hello, If the data was in .fastqsanger format, you could use the tool Manipulate FASTQ, but with .fasta, this is a good way. But watch your regular expression - test it out on a smaller set to make sure it is doing what you want. I see a start of the line character in the middle of your

Re: [galaxy-user] How to filter the sequences containing not[ATCG] character?

2013-12-09 Thread 朱师云
Hi, It indeed helps.Your regular expression looks brief and more useful.BTW, a start of line (^) between [] and in the first location, for example, [^ATCGatcg] means a character not [ATCGatcg], which maybe not work in the tool SELECT. Thank you for your help! Date: Mon, 9 Dec 2013 06:34:28

Re: [galaxy-user] How to filter the sequences containing not[ATCG] character?

2013-12-09 Thread Jennifer Jackson
Hello, You are right! I forgot about that. Aren't regular expressions fun? And please test it out, if you prefer your method or are just curious, I didn't try it that way. There are usually a few ways to do the same thing when using a regex. But, I am glad that this helped a bit and good luck