[galaxy-user] Illumina Methylation tool in toolshed

2013-07-29 Thread Sachit Adhikari
Hi,

This is my third post regarding this matter but however I didn't get any
reply. I am trying to find a tool for r Illumina Methylation. Is there any
available on the tool shed or test tool shed?

Thanks,

Sachit
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Re: [galaxy-user] Illumina Methylation tool in toolshed

2013-07-29 Thread Sachit Adhikari
Hi Bjoern,

Thank you for your reply.  To be precise I am looking for a wrapper of *
lumi. *http://www.bioconductor.org/packages/2.12/bioc/html/lumi.html

Do we have that in main tool shed or test tool shed or in github?

Thanks,

Sachit


On Mon, Jul 29, 2013 at 2:53 PM, Bjoern Gruening 
bjoern.gruen...@pharmazie.uni-freiburg.de wrote:


 Hi Sachit,

 not in the toolshed but you can find a start here:


 https://github.com/bgruening/galaxytools/tree/master/illumina_methylation_analyser

 its for the 450k chips, I have also some tools for WGMA if
 you are interested.

 Cheers,
 Bjoern

  Hi,
 
  This is my third post regarding this matter but however I
  didn't get any
  reply. I am trying to find a tool for r Illumina
  Methylation. Is there any
  available on the tool shed or test tool shed?
 
  Thanks,
 
  Sachit

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Re: [galaxy-user] Rename tool output file in Galaxy

2013-07-24 Thread Sachit Adhikari
Hi,

By i do you mean Edit attributes button? There are four sub-headings on
that: Attributes, Convert format, Datatype and permissions but I can't see
data file's full path on any of them. What's wrong?


On Wed, Jul 24, 2013 at 3:05 PM, Peter Cock p.j.a.c...@googlemail.comwrote:

 On Wed, Jul 24, 2013 at 5:21 AM, Sachit Adhikari
 sachit.techner...@gmail.com wrote:
  Hi group,
 
  The galaxy stores the output of the job on files/043/dataset_ID.dat.
 
  I have two questions here.:
 
  1) Can I find the ID of my output from the Galaxy history? I tried Edit
  Attributes, Annotations, tags but couldn't find it.

 Via the i icon of a history entry (information) you can see the
 datafile's full path which includes the ID.

  2) Can I rename my output while initiating the task? If I can how can I
 do
  this and what are the consequences.

 Yes, you can. Normally this is fine.

 (Note sure if there can be complications with meta-data normally
 added automatically by inspecting the finished file...)

 Peter

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Re: [galaxy-user] Rename tool output file in Galaxy

2013-07-24 Thread Sachit Adhikari
Yes, but unfortunately that didn't work too. I have already tried the
solution you provided. I could edit the tags and annotations but it won't
change the file name in my file system. The file is still named as *
dataset_43649.dat. *


On Wed, Jul 24, 2013 at 3:52 PM, Peter Cock p.j.a.c...@googlemail.comwrote:

 On Wed, Jul 24, 2013 at 10:35 AM, Sachit Adhikari
 sachit.techner...@gmail.com wrote:
  Hi,
 
  By i do you mean Edit attributes button? There are four sub-headings on
  that: Attributes, Convert format, Datatype and permissions but I can't
 see
  data file's full path on any of them. What's wrong?

 No, not the edit button.

 First click on a dataset's title so it expands. You should see a snippet
 of data etc plus a row of icons (save, information, reload in bottom
 left of the history, and tags and annotation bottom right).

 Peter

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Re: [galaxy-user] Rename tool output file in Galaxy

2013-07-24 Thread Sachit Adhikari
Thanks Peter for clearing this out.

On Wed, Jul 24, 2013 at 4:33 PM, Peter Cock p.j.a.c...@googlemail.comwrote:

 On Wed, Jul 24, 2013 at 11:15 AM, Sachit Adhikari
 sachit.techner...@gmail.com wrote:
  Yes, but unfortunately that didn't work too. I have already tried the
  solution you provided. I could edit the tags and annotations but it won't
  change the file name in my file system. The file is still named as
  dataset_43649.dat.

 You *cannot* rename the file on disk (without bypassing Galaxy
 and editing the database and renaming the file directly). This is
 by design - unless you are an administrator debugging something
 or a tool author you don't need to know the actual filename on disk.

 You *can* rename the description of the file as shown within the
 Galaxy interface (via edit attributes).

 Peter

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[galaxy-user] Rename tool output file in Galaxy

2013-07-23 Thread Sachit Adhikari
Hi group,

The galaxy stores the output of the job on files/043/dataset_*ID*.dat.

I have two questions here.:

1) Can I find the ID of my output from the Galaxy history? I tried Edit
Attributes, Annotations, tags but couldn't find it.

2) Can I rename my output while initiating the task? If I can how can I do
this and what are the consequences.

Thank you.


Regards,

Sachit
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[galaxy-user] Rename tool output file in Galaxy

2013-07-23 Thread Sachit Adhikari
Hi group,

The galaxy stores the output of the job on files/043/dataset_*ID*.dat.

I have two questions here.:

1) Can I find the ID of my output from the Galaxy history? I tried Edit
Attributes, Annotations, tags but couldn't find it.

2) Can I rename my output while initiating the task? If I can how can I do
this and what are the consequences.

Thank you.


Regards,

Sachit
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Re: [galaxy-user] Regarding galaxy wrappers: lumi, DESeq edgeR

2013-07-08 Thread Sachit Adhikari
Thank you Björn  and Ross. I will install the tools from the admin
interface.


On Mon, Jul 8, 2013 at 12:35 PM, Björn Grüning 
bjoern.gruen...@pharmazie.uni-freiburg.de wrote:

 Hi Sachit,

 we are working currently on a DESeq2 wrapper. Not yet finised sorry.
 There is also a edgeR Test repository from Ross Lazarus under:

 http://testtoolshed.g2.bx.psu.edu/view/fubar/edger_test

 Work is in progress, help is welcome!
 Ciao,
 Bjoenr

  Hi,
 
 
  These are the R libraries. Have anyone written a wrapper for these
  libraries lumi, DESeq edgeR?
 
 
  Thanks,
 
 
  Sachit
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[galaxy-user] Regarding galaxy wrappers: lumi, DESeq edgeR

2013-07-07 Thread Sachit Adhikari
Hi,

These are the R libraries. Have anyone written a wrapper for these
libraries lumi, DESeq edgeR?

Thanks,

Sachit
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[galaxy-user] hg19 reference gnome for Tophat2

2013-02-12 Thread Sachit Adhikari
I downloaded the entire directory of UCSC for the reference gnome of
Tophat2. It turns out that the Tophat2 and Bowtie2 uses the same reference
gnome. I found a directory: Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index

with the files:

genome.1.bt2  genome.2.bt2  genome.3.bt2  genome.4.bt2  genome.rev.1.bt2
 genome.rev.2.bt2


While adding the reference gnome, I need to edit bowtie2_indices.loc

Shall I replace:

/orig/path/hg19hg19hg19
 /depot/data2/galaxy/bowtie2/hg19/hg19

with

hg19   hg19hg19   Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index


Thanks,

Sachit
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Re: [galaxy-user] hg19 reference gnome for Tophat2

2013-02-12 Thread Sachit Adhikari
Also, do I have make all the reference files executable?

On Wed, Feb 13, 2013 at 6:39 AM, Sachit Adhikari 
sachit.techner...@gmail.com wrote:

 I downloaded the entire directory of UCSC for the reference gnome of
 Tophat2. It turns out that the Tophat2 and Bowtie2 uses the same reference
 gnome. I found a directory: Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index

 with the files:

 genome.1.bt2  genome.2.bt2  genome.3.bt2  genome.4.bt2  genome.rev.1.bt2
  genome.rev.2.bt2


 While adding the reference gnome, I need to edit bowtie2_indices.loc

 Shall I replace:

 /orig/path/hg19hg19hg19
  /depot/data2/galaxy/bowtie2/hg19/hg19

 with

 hg19   hg19hg19   Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index


 Thanks,

 Sachit

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[galaxy-user] Error in TopHat

2012-12-19 Thread Sachit Adhikari
This is the error I am getting while running Tophat. What's causing this? I
have the default hg19 index files.

Error in tophat:

[Tue Dec 18 10:08:33 2012] Beginning TopHat run (v1.3.3)
---
[Tue Dec 18 10:08:33 2012] Preparing output location ./tophat_out/
[Tue Dec 18 10:08:33 2012] Checking for Bowtie index files
Error: Could not find Bowtie index files
/opt/galaxyprod/galaxy-dist/data/hg19/hg19,/data/bowtie2-2.0.2/hg19.*

TopHat v1.3.3
TopHat v1.3.3
tophat -p 4
 /opt/galaxyprod/galaxy-dist/data/hg19/hg19,/data/bowtie2-2.0.2/hg19
/opt/galaxyprod/galaxy-dist/database/files/000/dataset_45.dat
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Re: [galaxy-user] Error in TopHat

2012-12-19 Thread Sachit Adhikari
And when I tried running tophat alone like this:

 tophat -r 20 test_ref reads_1.fq reads_2.fq


I get this error:

[2012-12-19 06:57:07] Beginning TopHat run (v2.0.7)
---
[2012-12-19 06:57:07] Checking for Bowtie
  Bowtie version: 2.0.2.0
[2012-12-19 06:57:07] Checking for Samtools
Samtools version: 0.1.18.0
[2012-12-19 06:57:07] Checking for Bowtie index files
Error: Could not find Bowtie 2 index files (test_ref.*.bt

On Wed, Dec 19, 2012 at 10:38 AM, Sachit Adhikari 
sachit.techner...@gmail.com wrote:

 This is the error I am getting while running Tophat. What's causing this?
 I have the default hg19 index files.

 Error in tophat:

 [Tue Dec 18 10:08:33 2012] Beginning TopHat run (v1.3.3)
 ---
 [Tue Dec 18 10:08:33 2012] Preparing output location ./tophat_out/
 [Tue Dec 18 10:08:33 2012] Checking for Bowtie index files
 Error: Could not find Bowtie index files
 /opt/galaxyprod/galaxy-dist/data/hg19/hg19,/data/bowtie2-2.0.2/hg19.*

 TopHat v1.3.3
 TopHat v1.3.3
 tophat -p 4
  /opt/galaxyprod/galaxy-dist/data/hg19/hg19,/data/bowtie2-2.0.2/hg19
 /opt/galaxyprod/galaxy-dist/database/files/000/dataset_45.dat


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[galaxy-user] How to add to add hg16 index into the build-in index / reference gnome list?

2012-12-12 Thread Sachit Adhikari
How do I add hg16 into the build-in index?
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[galaxy-user] Galaxy doesn't detect the file when we add dataset to a library under Manage data libraries

2012-12-11 Thread Sachit Adhikari
Hi list,

We have found that, in Galaxy, when we add dataset to a library under
Manage data libraries, Galaxy sometimes detects the type of the file and
sometimes does not. What I have noticed is that it detects the type of the
file when we select Copy file into Galaxy under Copy data into Galaxy?
option and does not detect it when we select Link to files ... Is this a
known issue? If it is, is there any workaround to solve the issue? Thanks

Regards,

Sachit
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[galaxy-user] How to add a built-in index for Tophat2?

2012-12-11 Thread Sachit Adhikari
TopHat2 needs built-in index/ reference genome in order for it to run. I
need to add hg 16 index into the build-in index / reference gnome list. I
already have the file. How can I add it?

Regards,
Sachit
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[galaxy-user] Problem in Manage data Library

2012-12-06 Thread Sachit Adhikari
We have found that, in Galaxy, when we add dataset to a library under
Manage data libraries, Galaxy sometimes detects the type of the file and
sometimes does not. What I have noticed is that it detects the type of the
file when we select Copy file into Galaxy under Copy data into Galaxy?
option and does not detect it when we select Link to files ... Is this a
known issue?

Regards,
Sachit
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[galaxy-user] Bismark Error: Data table named 'bismark_bs_indeces' is required by tool but not configured.

2012-11-29 Thread Sachit Adhikari
Hi all,
I am seeing this error while clicking on Bismark in Galaxy. I got the
Bismark for galaxy tool shed. I tried to get another bismark wrapper
developed by Bjoern. I didnt know how to download that. What's the error?
Thanks
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[galaxy-user] User's information

2012-11-29 Thread Sachit Adhikari
Where are the user's information stored in Galaxy? I can't find the
information in universe_wsgi.

Regards,
Sachit
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[galaxy-user] Getting error in Bismark

2012-11-01 Thread Sachit Adhikari
I am getting this error at the top while opening Bismark Mapper:
Data table named 'bismark_bs_indeces' is required by tool but not
configured.

I got Bismark from official toolshed.
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[galaxy-user] Data table named 'bowtie2_indexes' is required by tool but not configured

2012-10-19 Thread Sachit Adhikari
I am getting this error in Bowtie2 and Tophat2:
Data table named 'bowtie2_indexes' is required by tool but not configured
Data table named 'tophat2_indexes' is required by tool but not configured.

How can I solve it? Thanks
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Re: [galaxy-user] Data table named 'bowtie2_indexes' is required by tool but not configured

2012-10-19 Thread Sachit Adhikari
Thanks but after adding those lines when I stop and started the server
using --daemon mode, the server is not working? What might be the problem?

On Fri, Oct 19, 2012 at 9:01 AM, Jeremy Goecks jeremy.goe...@emory.eduwrote:

 You'll need to update the tool_data_table_conf.xml file in your Galaxy
 home directory.

 If you haven't made changes to the file, you can
 copy tool_data_table_conf.xml.sample to tool_data_table_conf.xml If you
 have made changes, add these entries to the file:

 --
 table name=bowtie2_indexes comment_char=#
 columnsvalue, dbkey, name, path/columns
 file path=tool-data/bowtie2_indices.loc /
 /table

 table name=tophat2_indexes comment_char=#
 columnsvalue, dbkey, name, path/columns
 file path=tool-data/bowtie2_indices.loc /
 /table
 --

 Finally, please direct questions about local Galaxy installations to the
 galaxy-dev mailing list: galaxy-...@bx.psu.edu

 Best,
 J.

 On Oct 19, 2012, at 2:58 AM, Sachit Adhikari wrote:

 I am getting this error in Bowtie2 and Tophat2:
 Data table named 'bowtie2_indexes' is required by tool but not configured
 Data table named 'tophat2_indexes' is required by tool but not configured.

 How can I solve it? Thanks
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[galaxy-user] Can't seem to stop or restart the Galaxy in server

2012-10-17 Thread Sachit Adhikari
Hello Everyone. Ctrl+C or Ctrl+D doesn't stop the Galaxy server. In local
machine, I need to close the terminal and restart the terminal again.
However, in server I integrated several tools, now I need to restart the
server to test it. I used ./run.sh --reload doesn't restart the server and
I can't stop the server. How can I stop or restart Galaxy in the real
working server? Thanks
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Re: [galaxy-user] Can't seem to stop or restart the Galaxy in server

2012-10-17 Thread Sachit Adhikari
Thanks. Helped me!

On Thu, Oct 18, 2012 at 11:02 AM, Enis Afgan eaf...@emory.edu wrote:

 With a certain version of Python there's been an issue stopping Galaxy
 using ctrl+C. You can use 'sh run.sh --daemon' to have the process run in
 the background and then 'sh run.sh --stop-damemon' to stop it.

 Hope this helps,
 Enis

 On Thu, Oct 18, 2012 at 4:12 PM, Sachit Adhikari 
 sachit.techner...@gmail.com wrote:

 Hello Everyone. Ctrl+C or Ctrl+D doesn't stop the Galaxy server. In local
 machine, I need to close the terminal and restart the terminal again.
 However, in server I integrated several tools, now I need to restart the
 server to test it. I used ./run.sh --reload doesn't restart the server and
 I can't stop the server. How can I stop or restart Galaxy in the real
 working server? Thanks
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[galaxy-user] Are tools must be installed in local machine?

2012-10-08 Thread Sachit Adhikari
Hello. The tools like Tophat, Bowtie etc are pre-installed in Galaxy. Are
those tools must be installed in order to work in Galaxy. For example: I am
using Debian machine(Don't have tophat and bowtie installed). Will tophat
and bowtie work in my local galaxy? Thanks
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Re: [galaxy-user] Are tools must be installed in local machine?

2012-10-08 Thread Sachit Adhikari
I don't think you understood my question very well. Tophat and Bowtie are
available by default in Galaxy. Tophat and Bowtie however is not installed
in my operating system. Will it still work in Galaxy?

On Mon, Oct 8, 2012 at 3:22 PM, Björn Grüning 
bjoern.gruen...@pharmazie.uni-freiburg.de wrote:

 Hi Sachit,

 you need to install such tools by yourself, unless you are using some
 new tools from the toolshed. For example the ncbi-tools or BWA from the
 toolshed are capable of installing all dependency automatically.
 In the future the plan is to move all heavy tools to the toolshed with
 automatic dependency and version handling, afaik.

 Regards,
 Bjoern

  Hello. The tools like Tophat, Bowtie etc are pre-installed in Galaxy.
  Are those tools must be installed in order to work in Galaxy. For
  example: I am using Debian machine(Don't have tophat and bowtie
  installed). Will tophat and bowtie work in my local galaxy? Thanks
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  at usegalaxy.org.  Please keep all replies on the list by
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  use the Galaxy Development list:
 
http://lists.bx.psu.edu/listinfo/galaxy-dev
 
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  please use the interface at:
 
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Re: [galaxy-user] RSEM on Galaxy

2012-10-05 Thread Sachit Adhikari
Hi there Alicia! To find the tool in the Galaxy tool search, first you have
to put your tool files in tools with .xml files then you have to edit
tool_conf.xml file. Here's the step by step tutorial:
http://wiki.g2.bx.psu.edu/Admin/Tools/Add%20Tool%20Tutorial

Regards,
Sachit

On Fri, Oct 5, 2012 at 8:55 PM, Victor ruo...@wisc.edu wrote:

 Hi Alicia,
 At the moment you have to install the wrapper manually. That is, the
 current version of the RSEM won't automatically install the rsem binaries
 for you. We are working on updating the tool from the tool shed. The readme
 file within the tarball provides instructions on what version of RSEM to
 install and other details. We ll keep you posted as soon as we update the
 tool so things will be done for you much like the other newer tools in the
 tool shed.
 Thank you for using RSEM and please let me know how it goes.

 Victor



 On Oct 5, 2012, at 7:14 PM, Ross ross.laza...@gmail.com wrote:

  Alicia,
 
  Some relevant details usually make it easier for list members to help
  you - please see
 
 http://wiki.g2.bx.psu.edu/Support#Public_mailing_list_Q_.26_A_discussions
 
  Did you load the offending tool from a toolshed or is it a tool
  wrapper you wrote yourself? If the latter, I have two suggestions:
 
  1) Search paster.log for your tool name and make sure it got correctly
  loaded when you restarted Galaxy - xml syntax errors like misplaced 
  characters will break the tool loading process.
 
  2) if the log shows that it loaded, make sure you refresh the tool
  frame in your browser after restarting Galaxy - eg clicking the
  analyse data tab will get rid of the old cached copy.
 
 
  On Sat, Oct 6, 2012 at 10:02 AM, Alicia R. Pérez-Porro
  alicia.r.perezpo...@gmail.com wrote:
  Hi all,
 
  Recently i installed RSEM into Galaxy.
  The problem is that now i cannot find the tool when i open my galaxy
 in my
  computer. I checked and rsem folder is inside the tool folder.
  Any suggestions?
 
  Thanks,
  Alicia.
 
 
 
 
 
  --
  Alicia R. Pérez-Porro
  PhD student
 
  Giribet lab
  Department of Organismic and Evolutionary Biology
  MCZ labs
  Harvard University
  26 Oxford St, Cambridge MA 02138
  phone: +1 617-496-5308
  fax: +1 617-495-5667
  www.oeb.harvard.edu/faculty/giribet/
 
  Department of Marine Ecology
  Center for Advanced Studies of Blanes (CEAB-CSIC)
  C/Accés Cala St. Francesc 14
  17300 Blanes, Girona, SPAIN
  phone: +34 972 336 101
  fax: +34 972 337 806
  www.ceab.csic.es
 
 
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  --
  Ross Lazarus MBBS MPH;
  Head, Medical Bioinformatics, BakerIDI; Tel: +61 385321444
  http://scholar.google.com/citations?hl=enuser=UCUuEM4J
 
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[galaxy-user] Trim Galore Galaxy Integration

2012-10-04 Thread Sachit Adhikari
Hello. I would like to integrate this great tool Trim Galore in the local
Galaxy. Has anyone did this before? If so, please provide me with the
wrapper and  .xml file? How can I integrate it?

Best wishes,
Sachit
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[galaxy-user] Trim Galore Galaxy Integration

2012-10-04 Thread Sachit Adhikari
Hello. I would like to integrate this great tool Trim Galore in the local
Galaxy. How can I do that? Thanks in advance.
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