Re: [galaxy-user] Text Manipulation

2011-02-22 Thread Peter Cock
On Tue, Feb 22, 2011 at 2:28 AM, Jennifer Jackson wrote: > Hi Felix, > > "Text Manipulation -> Convert delimiters to TAB" could split one field into > more than one, but the delimiter has to be in the list ("@" is not). > > "Text Manipulation -> Cut columns" from a table is similar, but it will no

Re: [galaxy-user] Text Manipulation

2011-02-22 Thread Felix Hammer
Hi Peter, sounds nice, would be a great feature. For everyone else how is not using a custom server: if you are lucky you can use the trim tool on tabs to solve your problem. If you want to add text to the beginning or end of a column: - Use add column and add the text as a new column - Then use

[galaxy-user] Group and hide steps in history/workflow

2011-02-22 Thread Felix Hammer
Hi, it would be really nice if I could take a work flow and save it as a tool. This way one could easily build custom text-manipulation tools from already existing text tools. I already have a work flow that does a conversion job. But it has lots of of steps that make my histories quite messy when

Re: [galaxy-user] Group and hide steps in history/workflow

2011-02-22 Thread Peter Cock
On Tue, Feb 22, 2011 at 11:40 AM, Felix Hammer wrote: > Hi, > > it would be really nice if I could take a work flow and save it as a tool. > This way one could easily build custom text-manipulation tools from > already existing text tools. I already have a work flow that does a > conversion job. B

Re: [galaxy-user] Group and hide steps in history/workflow

2011-02-22 Thread Felix Hammer
I've done that. The intermediate outputs are hidden. But all the steps are still displayed. I want to hide those, too. Felix > On Tue, Feb 22, 2011 at 11:40 AM, Felix Hammer > wrote: >> Hi, >> >> it would be really nice if I could take a work flow and save it as a >> tool. >> This way one could

Re: [galaxy-user] Group and hide steps in history/workflow

2011-02-22 Thread Peter Cock
On Tue, Feb 22, 2011 at 1:23 PM, Felix Hammer wrote: > I've done that. The intermediate outputs are hidden. > But all the steps are still displayed. I want to hide those, too. > > Felix I'm unclear which bit(s) you want to hide in the GUI. With the recent enhancements, the "run workflow" dialogu

Re: [galaxy-user] Group and hide steps in history/workflow

2011-02-22 Thread Felix Hammer
Ah, I've figured it out now :) thx. Felix > I've done that. The intermediate outputs are hidden. > But all the steps are still displayed. I want to hide those, too. > > Felix > > >> On Tue, Feb 22, 2011 at 11:40 AM, Felix Hammer >> wrote: >>> Hi, >>> >>> it would be really nice if I could take a

Re: [galaxy-user] Generating pileup for SNP analysis

2011-02-22 Thread Jennifer Jackson
Hello Christine, The data issue goes back to the initial grooming step. When using "Fastq Groomer", choose "Solexa" instead of "Illumina 1.3+" as the Input FASTQ quality score type. This preserves the correct quality score translation, which was the root of the problem in the derivative steps.

Re: [galaxy-user] get wig file after tophat

2011-02-22 Thread Ann Loraine
I'm a fan of viewing "coverage.wig" files - they give a nice summary of read distribution over a chromosome. What I like about the "coverage.wig" file is that it captures the full read depth at every base. TDF does something different - it summarizes over (relatively) large regions. (But this can

Re: [galaxy-user] get wig file after tophat

2011-02-22 Thread David Matthews
HI, The option you need in IGV tools is "count". You set a window size and this gives you a tdf file from your sorted bam (or sam) file which is nice and quick to view on IGV. Best Wishes, David. __ Dr David A. Matthews Senior Lecturer in Virology Room E49 Dep

Re: [galaxy-user] get wig file after tophat

2011-02-22 Thread vasu punj
David,   I have been also uisng the IGV like you have suggested.   Vasu --- On Tue, 2/22/11, David Matthews wrote: From: David Matthews Subject: Re: [galaxy-user] get wig file after tophat To: "Ying Zhang" Cc: "Baxter, Adam" , galaxy-u...@bx.psu.edu Date: Tuesday, February 22, 2011, 10:54 AM

Re: [galaxy-user] 2011 Galaxy Community Conference, May 25-26, Lunteren, The Netherlands

2011-02-22 Thread Dave Clements
Hello all, Just a reminder that the abstract submission deadline for the Galaxy Community Conference is next Monday, February 28. See http://galaxy.psu.edu/gcc2011/Abstracts.html for details. Cheers, Dave C. PS: And there is still space at the GMOD Meeting being held March 5-6 @ NESCent: htt

Re: [galaxy-user] get wig file after tophat

2011-02-22 Thread Jeremy Goecks
Ying, You can create a coverage file from a BAM file using this workflow: http://main.g2.bx.psu.edu/u/jeremy/w/create-coverage-dataset-from-bam-dataset While this doesn't generate a proper wiggle file, it can be a useful file for statistical analysis. I and others have suggested ways to visual

Re: [galaxy-user] Generating pileup for SNP analysis

2011-02-22 Thread Jennifer Jackson
Hello Christine, We have confirmed that the reads have Sanger qual format, not Illumina (as were originally run in your history) or Solexa (as some are at NCBI, from other SRA projects, but not this one). Please re-run the Fastq groomer with Sanger as the input type and all should run correctl

Re: [galaxy-user] fastx_clipper.xml and FASTQ Illumina 1.3+?

2011-02-22 Thread Jennifer Jackson
Hi Peter, For now we are going to recommended just using the Fastq Groomer first, then the Clipper, to keep the tools set simple. Apologies for the late reply, we were discussing the option internally. Thanks for all the great ideas and contributions! Best, Jen Galaxy team On 1/6/11 4:28 A

Re: [galaxy-user] [galaxy-dev] Peep view for history elements broken on IE?

2011-02-22 Thread Kanwei Li
Hi Peter, The error when clicking tool menu links is now fixed on galaxy-central tip. Thanks for reporting! On Mon, Feb 21, 2011 at 4:48 AM, Peter wrote: > > On Fri, Feb 18, 2011 at 3:13 PM, Kanwei Li wrote: > >> > >> All: > >> I retested under IE7 and found the bugs you were mentioning. This

[galaxy-user] Fwd: normalizing for number of tags

2011-02-22 Thread Jennifer Jackson
Hi Nadege, I am forwarding your question to galaxy-u...@bx.psu.edu which is the best place to send data questions, so that the entire team can provide input. We will get back to you as soon as possible. Thanks! Jen Galaxy team --- Hi Jen, I am completely new to the field of ChIP-seq and h