[galaxy-user] FASTA Generation from Pileup for SNP Detection

2011-02-25 Thread John David Osborne
I'm interested in generating a fasta file from Ilumina paired reads of my wild type strain. I have an NCBI reference genome I can assemble against and I have already uploaded my 2 reads (using the NCBI reference as my genome), used fastq groomer, aligned them with bowtie and generated my pileup.

[galaxy-user] de-novo motif analysis

2011-02-25 Thread Christopher Futtner
Two questions - 1. When I call peaks with MACS on Galaxy I get different results than if I run MACS on my machine via command line. I'm using default parameters in both instances. 2. Are there any tools in galaxy for doing de-novo motif analysis of the peaks that I have called. If not can anyone su

[galaxy-user] de-novo motif analysis

2011-02-25 Thread Christopher Futtner
Two questions - 1. When I call peaks with MACS on Galaxy I get different results than if I run MACS on my machine via command line. I'm using default parameters in both instances. 2. Are there any tools in galaxy for doing de-novo motif analysis of the peaks that I have called. If not can anyone su

Re: [galaxy-user] de-novo motif analysis

2011-02-25 Thread vasu punj
If it is ChIP-motif you may like to consider http://motif.bmi.ohio-state.edu/ChIPMotifs/ There are several others but this is my favorite.   Vasu Punj --- On Fri, 2/25/11, Christopher Futtner wrote: From: Christopher Futtner Subject: [galaxy-user] de-novo motif analysis To: galaxy-u...@bx.psu