Re: [galaxy-user] galaxy-user Digest, Vol 91, Issue 21

Hi, I just notice that NGS FASTQ Trimmerby column can't detect the fastq file I loaded. Anyone knows why. thanks a lot. On Fri, Jan 24, 2014 at 11:00 AM, wrote: > Send gala

[galaxy-user] NGS Fastq Trimmer error

Hi Wang, please check if your fastq file is associated with the correct fastq format, fastqsanger probably. Cheers, Bjoern > Hi, > > I just notice that NGS FASTQ Trimmer by column can't detect the fastq > file I loaded. Anyone knows why. thanks a lot. > > > > On Fri, Jan 24, 2014 at 11:00 AM

Re: [galaxy-user] NGS Fastq Trimmer error

The fastq format is correct, when i use FASTQ to FASTAconverter, the fastq file can be detected. On Thu, Feb 13, 2014 at 1:14 PM, Björn Grüning wrote: > Hi Wang, > >

Re: [galaxy-user] NGS Fastq Trimmer error

By the way, can you use detect the fastq file using trimmer by column with your PC. Thanks a lot. Gang On Thu, Feb 13, 2014 at 1:14 PM, Björn Grüning wrote: > Hi Wang, > > please check if your fastq file is associated with the correct fastq > format, fastqsanger probably. > > Cheers, > Bjoern

Re: [galaxy-user] NGS Fastq Trimmer error

Hi Wang, The assigned format is the issue, as Björn replied. The two tools you are referencing to have different criteria for the input fastq data - one is more stringent than the other with regard to /_content_/. Both are fastq /_format_/, but the one that requires the more specific fastqsan

Re: [galaxy-user] NGS Fastq Trimmer error

Hi Gang, I can't comment about a PC (I use a MAC), but this shouldn't matter. If you are using a local Galaxy (http://getgalaxy.org http://usegalaxy.org/toolshed), most tools will follow the same usage rules as when implemented on the public Main Galaxy instance at http://usegalaxy.org, the G

[galaxy-user] Bisulfite seq

Do we have any tool which can be used for analyzing and QC check for bisulfite sequencing. I have FASTq files. Thanks Mathew___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public serve

Re: [galaxy-user] NGS Fastq Trimmer error

Hi Jennifer, I reload my data with defined format type fastqsanger and it works now. Thank you so much for your help. Gang On Thu, Feb 13, 2014 at 6:06 PM, Jennifer Jackson wrote: > Hi Gang, > > I can't comment about a PC (I use a MAC), but this shouldn't matter. If > you are using a local Ga