Re: [galaxy-user] GTF-to-GFF3

2011-03-22 Thread Florent Angly
Speaking of which, could a Galaxy developer look at adding these changes, please? https://bitbucket.org/galaxy/galaxy-central/issue/447/new-tools-to-de-interlace-fastq-mate-pair Thanks, Florent On 23/03/11 12:02, Jeremy Goecks wrote: Alternatively, we welcome community contributions to the

[galaxy-user] Fwd: Workflows with conditional statements

2011-05-21 Thread Florent Angly
Date: Wed, 18 May 2011 10:31:21 +1000 From: Florent Angly florent.an...@gmail.com To: galaxy-user@lists.bx.psu.edu galaxy-user@lists.bx.psu.edu Hi all, I was wondering if there is a way to put conditional statements in a Galaxy workflow. This would be useful, for example, in the case

Re: [galaxy-user] filtering fastq file according to qual score

2011-07-30 Thread Florent Angly
Hi Haluk, By filtering, you mean removing reads? trimming their ends? or masking some of their bases? There are 3 tools under Generic FASTQ manipulation that may help you: Filter FASTQ reads by quality score and length FASTQ Quality Trimmer by sliding window FASTQ Masker by quality

Re: [galaxy-user] filtering fastq file according to qual score

2011-07-31 Thread Florent Angly
for removing bases. After your reply, I got suspicious. Am I trying to do something wrong in theory? Thanks in advance. On Sun, Jul 31, 2011 at 6:07 AM, Florent Angly florent.an...@gmail.com mailto:florent.an...@gmail.com wrote: Hi Haluk, By filtering, you mean removing reads? trimming

Re: [galaxy-user] Simulating sequencing and removing redundant sequences

2011-09-20 Thread Florent Angly
For read simulation, you may also want to give Grinder a try. I made a Galaxy wrapper for it (see in the toolshed: http://toolshed.g2.bx.psu.edu/) Florent On 20/09/11 18:46, Kevin Lam wrote: Hi Daniel, You would have multiple names for each sequence and that would be quite hard to display. I

Re: [galaxy-user] Problem with new tool shed

2011-10-04 Thread Florent Angly
additional problems. Thanks for finding this! Greg Von Kuster On Oct 4, 2011, at 2:53 AM, Florent Angly wrote: Hi all, I tried the latest stable version of Galaxy: http://wiki.g2.bx.psu.edu/News%20Briefs/2011_08_30. This page has links to how to use the new tool shed including how

Re: [galaxy-user] Patch for better FASTQ description handling

2011-10-18 Thread Florent Angly
I have had the chance to try the patch on several datasets and it looks good :) I reiterate my suggestion to pull the patch in galaxy-central. Best, Florent On 05/10/11 18:28, Florent Angly wrote: Hi, I have found some issue with the way FASTQ read description is handled by Galaxy

Re: [galaxy-user] Patch for better FASTQ description handling

2011-10-19 Thread Florent Angly
Peter and Daniel, thanks for the comments. On 19/10/11 23:49, Peter Cock wrote: On Wed, Oct 19, 2011 at 2:31 PM, Daniel Blankenbergd...@bx.psu.edu wrote: Hi Florent, Sorry for the delay. I did try the patch out shortly after you contributed it, but it caused the functional to fail. I was

Re: [galaxy-user] FASTQ Quality Trimmer for PE Illumina?

2011-10-25 Thread Florent Angly
Hi Joanna, During trimming, some of the reads may be removed from your dataset. Depending on what you need to do, you may or may not want to discard reads that don't have a mate mate anymore. If so, you might consider using the FASTQ de-interlacer and interlacer tools. Florent On 26/10/11

Re: [galaxy-user] Patch for better FASTQ description handling

2011-11-29 Thread Florent Angly
On 19/10/11 23:31, Daniel Blankenberg wrote: Sorry for the delay. I did try the patch out shortly after you contributed it, but it caused the functional to fail. I was able to fix the issue and allow the existing tests to start passing, but I've been bogged down lately and haven't been

[galaxy-user] Recent problematic change in the Galaxy Toolshed

2012-01-17 Thread Florent Angly
Hi, I just tried to upload a new version of the Grinder Galaxy wrapper to the Galaxy ToolShed and am encountering difficulties that I did have in previous versions. When I upload my .tar.gz file, the upload is aborted and I get the error: 'GalaxyWebUITransaction' object has no attribute

Re: [galaxy-user] Recent problematic change in the Galaxy Toolshed

2012-01-19 Thread Florent Angly
instance with no problems, so I believe your grinder repository is now in a good state. Thanks very much for reporting this issue. Greg Von Kuster On Jan 17, 2012, at 8:43 PM, Florent Angly wrote: Hi, I just tried to upload a new version of the Grinder Galaxy wrapper to the Galaxy ToolShed