[galaxy-user] How to show sequence length data

2012-11-08 Thread Zhiqiang Shu
Hi, there I'm new to this field and have a question when using Galaxy. I have RNA sequence data. How can I plot the graph of the length of sequence with Galaxy. Thanks! ___ The Galaxy User list should be used for the discussion of Gal

[galaxy-user] Find out sense and antisense sequences

2012-11-26 Thread Zhiqiang Shu
Hi, Galaxy users! I have a question on how to find out sense and antisense sequence. I've got RNA seq data in the fastq format. The sequences inside are partially complementary to each other (complementary is 10nt, while entire is about 30nt). How can I separate these sequences into two g

Re: [galaxy-user] Find out sense and antisense sequences

2012-11-26 Thread Zhiqiang Shu
group, they share the feature of having an "A" at their 10th. In this case, how can I deal with it? One possible way come up is inverting all sequences and aligning them. Thanks! Best, Zhiqiang Quoting "Peter Cock" : On Mon, Nov 26, 2012 at 6:47 PM, Zhiqiang Shu wro

Re: [galaxy-user] Find out sense and antisense sequences

2012-11-27 Thread Zhiqiang Shu
ice (and more complicated). Tools in "Interval Operation" group will be of help if you go down that path. Best, Jen Galaxy team On 11/26/12 10:47 AM, Zhiqiang Shu wrote: Hi, Galaxy users! I have a question on how to find out sense and antisense sequence. I've got RNA seq

Re: [galaxy-user] combining two fastq files

2012-11-27 Thread Zhiqiang Shu
ns to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ ---------- Zhiqiang Shu/Deng Lab Department of Biological Science Florida State University 319 Stadium Dr. Tallahassee, FL, USA, 32306-4

[galaxy-user] trim adapters and other

2012-11-29 Thread Zhiqiang Shu
Hi, all! I have a maybe naive question that might be not so related to Galaxy usage. So I got the RNA seq data from Illumina Hiseq. Do I need to get rid of the adapter manually? If so, is it "clip adapter" that I should use? It should only have one adapter sequence or maybe more than one?

[galaxy-user] order of steps

2012-12-05 Thread Zhiqiang Shu
Hi, I have two simple questions related to manipulating step history. So, how to change the order of steps, for example, put step 7 right after step 3. Second question is related, how to re-order steps, for instance, I deleted step 4 and 5 and want the next step (step 6) to take the order