Hello,
I'm new to Galaxy.
When I read in a fasta file to Galaxy and then try to use it (in a blast
search) as the query sequence, I get the error message below, although
the uploaded fasta file is present in the history. Can anyone tell me
what the problem could be? Is there some other step I
Hello Kenneth,
Are you using BLAST+ in a local install or cloud instance? The problem
may be that the query dataset needs to have the datatype assigned as
fasta. To do this, click on the pencil icon for the dataset to reach
the Edit Attributes form. Then either scroll down to (or click on the
Hi Kenneth,
Yes, target databases require indexes and *.loc file set-up. Please see
this wiki for details. For Genbank data such as NR, FTP the pre-built
indexes and use those (generating them with formatdb is not necessary).
http://wiki.g2.bx.psu.edu/Admin/NGS%20Local%20Setup
See the
Hi Kenneth,
It is likely that the path is wrong in the .loc file. It has to point to
the actual files, not just the directory. Inside here
/9720/genome_references/ncbi/nr-protein-db
is where all the nr.* files are? In that case, the path should be
4 matches
Mail list logo
