Re: [galaxy-user] Running Cufflinks on Bacterial RNAseq data

2012-08-01 Thread Jennifer Jackson
Peter is correct (I oversimplified)! And Cufflinks does allow for an ID attribute to span lines as long as it represents the same feature. To be clear, this error was a true format issue. The best way to understand the finer points is to see the specification (also linked from wiki below): h

Re: [galaxy-user] Running Cufflinks on Bacterial RNAseq data

2012-08-01 Thread Peter Cock
On Tue, Jul 31, 2012 at 7:35 PM, Jennifer Jackson wrote: > Hello Rachel, > > When datasets are in a grey "waiting to run" state this indicates that they > are in the queue and in line to run. For the majority of cases, including > yours, leaving the job alone and allowing it to run is the correct

Re: [galaxy-user] Running Cufflinks on Bacterial RNAseq data

2012-07-31 Thread Jennifer Jackson
Hello Rachel, When datasets are in a grey "waiting to run" state this indicates that they are in the queue and in line to run. For the majority of cases, including yours, leaving the job alone and allowing it to run is the correct option. The missing metadata only means that the result has not

[galaxy-user] Running Cufflinks on Bacterial RNAseq data

2012-07-27 Thread Rachel Krasich
I am attempting to run Cufflinks on Galaxy main to analyze my E. coli RNAseq data. I have mapped my reads using an outside program (Genious) and uploaded the resulting BAM file. I also have uploaded the E. coli annotations as a gtf file. However when I attempt to run Cufflinks using my annotatio