Try your eosin stain used in your H or 1% Neutral red in 2% acetic acid
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax:
Good afternoon,
Does anyone know the correct subspecialty for Buffy Coats on the CLIA
certificate?
Any feedback would be appreciated!
Caroline M. Pratt, MBA
Practice Administrator Dermpath
3020 Market Street, Ste 201
Philadelphia, PA 19104
Phone 215-349-8178
Cell 610-800-1381
Fax
HI Tasha,
The nuclear fast red will stain the nuclei red, the biebrich scarlet is a
connective tissue stain and may cover up the (sometimes sparse) prussian
blue localization of iron in the tissues. I, personally, would not consider
these reagents swappable.
In my experience nuclear fast red
Oops, yes, I am mixing up neutral red - and the recipe from Bryan is what I
use - it last forever.
I have had luck with nuclear fast red from vector - comes made up. That
lasts a little longer than lab made.
mills
ᐧ
On Wed, Aug 8, 2018 at 12:52 PM Caroline Miller wrote:
> HI Tasha,
>
> The
1% neutral red in 0.01% acetic acid (1 mL of 1% per 100 mL stain) works,
as does 1% safranin. Both are stable, although should be filtered from
time to time. Stain 1 minute, water rinse, ethanol, xylene and mount
should work. I had one pathologist who preferred a very light
progressive H She
Tasha Campbell, B.S.,HTL(ASCP), at Frederick Gastroenterology Associates in
Frederick, Maryland asks:
>>Is nuclear fast red the only counter stain for the Prussian blue stain? I
have a Masson's trichrome kit and was wondering if [Biebrich] scarlet could
be [used as] a counterstain. I won't be
Is nuclear fast red the only counter stain for Prussian blue stain? I have a
Masson's trichrome kit and was wondering if the Bierbech Scarlet could be a
counterstain. I won't be doing the iron stain very often at all so I am trying
to keep dry reagents on hand to make up as needed so they do
