Do you mean between specimens or per shift?
Thanks
Cristi
Sent from my iPhone
> On Feb 26, 2020, at 5:24 PM, Charles Riley via Histonet
> wrote:
>
> How often does everyone clean their water-bath?
>
> --
>
> Charles Riley BS HT, HTL(ASCP)CM
>
> Histopathology Coordinator/ Mohs
>
How often does everyone clean their water-bath?
--
Charles Riley BS HT, HTL(ASCP)CM
Histopathology Coordinator/ Mohs
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Are you using some form of antigen retrieval? In addition to what has been
discussed previously, antigen retrieval using heat (HIER) can cause tissue loss
especially collagen.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic
Hi Histonetters,
How are you doing today?
This is an exciting week for me!
This week we are celebrating
RELIAs 15th year in business.
15 years, 180 months, 782 weeks, 5,475 days, 131,400 hours and loving EVERY
one of the 7,884,000 minute of it!
RELIA Solutions 2/2005-2/2020 Cheers to 15 more!
Hi Charles,
It adhesion problems can arise from several sources, some have already been
mentioned. Here are two that I have experienced:
1) Bad slides. Either a manufacturing defect such that the positive charge
is insufficient or the slides were somehow compromised after opening them
on the bench
In my experience;
If the the specimen is under-fixed or under-processed, the section will be
sloughed off during IHC staining.
I find proper fixation time and drying the slides at room temperature (before
baking them in oven) optimal.
Andy
Abdulbasit Andijany (Andy)
AMLS, BBMS, MSc, CT
Do you float and pick up your sections from a water bath of distilled water
with no adhesive added? If not, doing that will help.
John
On Wed, Feb 26, 2020 at 6:41 AM, Charles Riley via Histonet
wrote:
> Our pathologists are complaining that chunks of the dermis are missing from
> IHC slides
Our pathologists are complaining that chunks of the dermis are missing from
IHC slides yet the entire section is present prior to staining.
Does anyone have any ideas what could cause the tissue to not adhere to the
slides throughout the staining process? We use the Leica Bond stainers.
--
I know this topic has probably been addressed many times before and I do not
wish to start the certified vs non certified debate again. However, I must ask
for those of you who have both working in your lab, do their job descriptions
and responsibilities differ between those who are certified
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