Re: [Histonet] IHC with zamboni's fixed tissues

Hi Colleen, I was involved in an Immunohistochemical study of the nerves in the human ureter that used Zamboni's fixative and cryostat sections. This study was before the invention of HIER. If you are using Zamboni-fixed, paraffin embedded tissue then you will probably need to do HIER before

[Histonet] IHC with zamboni's fixed tissues

Hello Histonet, Can you give me feedback on trying to do IHC on samples fixed in Zamboni's fixative? I have an idea but need confirmation . Thank you in advance Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455

Re: [Histonet] New antibody diluent

CAP specifically addresses Scott's question in the checklist (see below, copied from the Checklist) ANP.22780 Laboratories confirm assay performance when conditions change that may affect performance. NOTE: A change in antibody clone requires full revalidation/verification of the assay

[Histonet] Changing decal question

Good afternoon, I would like to inquire what everyone's decal (RBD and/or Decal I) changing routines are. We are trying to determine if we should continue to change weekly, or based on how many specimens we put in it, or what the pH is. Any help is greatly appreciated, thanks! Jennifer Wooten,

[Histonet] RELIA HOT Histology Opportunity Update - RELIA EXCLUSIVES!!

Hi Histopeeps! I hope you are having a great week. I wanted to drop a quick post because I have some really exciting opportunities to share. Why are they exciting? 1. Beautiful locations 2. Day shift hours 3. Excellent compensation packages 4. They are ready to interview and

[Histonet] Positive control blocks for Helicobacter pylori

Good morning. I would like to obtain positive control blocks for Helicobacter pylori. Thanks in advance for your help. -- Rhonda Ford, Histology Lab Henry Community Health Main Campus 1000 North 16th Street New Castle, Indiana 47362 (765) 521-1148 -- *"We Make Lasting Connections"*Henry

Re: [Histonet] Antibody diluent

I’m not completely sure but I think you can do either a mini validation or a comparison validation to show there’s no difference between the results. I think CAP has a requirement for what to do when reagents change. Paula Sent from my iPhone > On Mar 25, 2021, at 6:19 AM, Jeanine

[Histonet] Mailing List

Hello, Kindly remove me for the Histonet mailing list. I've attempted several times with no success. Thank You, *Carolyn Baumann MT(ASCP)* Regional Product Specialist-Molecular Diagnostics Pacific Northwest/Mountain Region Roche Diagnostics Corporation Molecular Diagnostic Sales 9115 Hague

Re: [Histonet] Antibody diluent

Scott:I’m pretty certain that you wouldn’t need to completely re-validate after changing diluents, but you may have to change the dilution factor slightly.   It’s my understanding that a change like this only requires staining a few known positive samples, verifying that the results you obtain

Re: [Histonet] Antibody diluent

Hi Scott, It may be best to call CAP to get an interpretation. However, I think that since you would be changing diluent for predictive markers, a full validation may be required. Best, Dan Daniel Pesino, HTL(ASCP)QIHCCM Senior Histotechnologist Trinity Health Of New England

Re: [Histonet] Antibody diluent

What is everyone's take on what needs to be done from a verification/validation standpoint if you switch diluents for your concentrated antibodies? The CAP summary of recommendations on the validation IHC antibodies doesn't specifically mention the changing of diluent as one of the variables.