[Histonet] PEGsylated cells

etc.. Since this is a new concept for me I am reaching out to see if anyone in this group has worked with PEG and can give me references, diorection, protocols... As always, thanks in advance~. Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St

Re: [Histonet] Agarose embedded tissue arrays embedded in paraffin block

with the agar it will not have [processed properly. I was never able to salvage those samples. It took me a couple times to figure out the solution. Just a thought. Colleen Forster HT(ASCP)QIHC On Fri, May 17, 2024 at 2:32 PM Jay Lundgren via Histonet < histonet@lists.utsouthwestern.edu>

Re: [Histonet] Ihc control tissues

if interested in more information. Colleen Forster HT(ASCP)QIHC cfors...@umn.edu On Wed, May 8, 2024 at 9:56 AM Charles Riley via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Awesome thank you Val. I like the multi control option. Can save money in > the end by using l

Re: [Histonet] [EXT] Reagent alcohols and tissue processors

for that. I am fortunate to have a second processor I can use for special programs and not hold up my main runs. Colleen Forster HT(ASCP)QIHC University of Minnesota BLS Histology and IHC Laboratory On Thu, Mar 28, 2024 at 9:15 AM Paula Sicurello via Histonet < histonet@lists.utsouthwestern.

Re: [Histonet] Modified Davidson solution

I agree. Making some of these simple solutions that need to be FRESH is the better way to go. As John said, there are ma ny books with these formulas in them. I have used Davidson's for different projects and I make mine in the lab fresh each time tissue is harvested. Colleen Forster HT(ASCP

Re: [Histonet] Microtome block holder

You are very welcome! Colleen On Tue, Nov 7, 2023 at 4:02 PM Naira Margaryan wrote: > Awesome, thank you so much Colleen, for such quick response, > Naira > > On Tue, Nov 7, 2023 at 4:01 PM Colleen Forster wrote: > >> Naira, >> >> Contact Lee Dickey with Ted

Re: [Histonet] Microtome block holder

Naira, Contact Lee Dickey with Ted Pella. They carry the whole line of large block adapters for the Leica microtomes. lee_dic...@tedpella.com I have this set up and it works great. Colleen Forster HT(ASCP)QIHC On Tue, Nov 7, 2023 at 3:58 PM Naira Margaryan via Histonet < histo

Re: [Histonet] LEAD HISTOTECHNOLOGIST OPPORTUNITY

I would love too but unable to move the whole family! Colleen Forster On Fri, Oct 27, 2023 at 11:20 AM Stephanie L. Thompson via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Come to beautiful New Hampshire, the best beaches, Great White Mountains, > no income or state ta

Re: [Histonet] maximum thickness brain in slide

Even 30um is too thick for the routine IHC on glass. You will never penetrate the whole thickness for staining. Colleen Forster HT(ASCP)QIHC On Fri, Aug 4, 2023 at 2:05 PM Alonso Martínez Canabal < acana...@ciencias.unam.mx> wrote: > Hi, thank you for your reply. > I cannot do m

Re: [Histonet] maximum thickness brain in slide

Alonso, For sections that thick you would collect them as you always have, stain them as floating sections and then mount onto a glass slide. The routine standard methods for IHC will never penetrate a 50um thick section. Colleen Forster HT(ASCP)QIHC On Fri, Aug 4, 2023 at 1:17 PM Alonso

Re: [Histonet] shelf life of working antibody solutions in IHC

I run a research lab with automated staining and truthfully,I make my dilutions fresh for each run because proteins are so different in their stability. Colleen Forster HT(ASCP)QIHC On Sun, Jul 2, 2023 at 7:08 PM Tony Henwood via Histonet < histonet@lists.utsouthwestern.edu>

[Histonet] Changing solutions on processors and H/E stainers

for comparison. I am aware of the many variables. For better comparison to my lab we use formalin and xylenes not a substitute. Any and all infirnation will be includedThank you very much in advance. Respectfully, Colleen Forster -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory

Re: [Histonet] Immunoflouresence staining

In my experience if the antibody are designed for flow they rarely work on tissue samples. Colleen Forster HT(ASCP)QIHC University of MN On Wed, Aug 24, 2022 at 1:56 PM Charles Riley via Histonet < histonet@lists.utsouthwestern.edu> wrote: > I have been given an antibody that is used

Re: [Histonet] Asking for software

Donna, I know first hand, if you have any computer issues that take out your software, ( power surge for example) Leica does NOT reinstall the program. You are left to find a different program. It took us a long time to get a program so we could use both the IPC/IPS again. The new software isn't

[Histonet] slide printers

some of you share what you have and the pros/cons? I need to start the search for the replacement I would like. Thank you in advance. -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930

Re: [Histonet] Super cold cryostat cutting head temperatures

ith specific > cryo attachments is used. > > Sincerely, > > Paula Sicurello > > Sent from my iPhone > > > On May 18, 2022, at 12:55 PM, Colleen Forster via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > > FollowingI am in r

Re: [Histonet] Super cold cryostat cutting head temperatures

FollowingI am in research and I have never cut this cold. Colleen Forster On Wed, May 18, 2022 at 2:04 PM Ken Marzinsky via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Can anyone tell me why research labs often require cryostat cutting head > temperatures down to -

Re: [Histonet] Advice for new grads?

Great advice Tim. I still do this 30 years later. It definitely expands your scope of work, keeps the job interesting and builds your resource network Colleen Forster HT(ASCP)QIHC On Mon, May 9, 2022, 5:38 PM Tim Morken via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Pa

Re: [Histonet] Jones' Methenamine Silver Stain for Basement Membranes of Kidney - Issues and Questions

Make sure the periodic acid is made fresh EACH time you run the stain. That can also make a big difference in the stain quality. Colleen Forster HT(ASCP)QIHC On Thu, Sep 23, 2021 at 6:14 PM Tony Henwood (SCHN) via Histonet < histonet@lists.utsouthwestern.edu> wrote: > I agree w

Re: [Histonet] Cryostat

I agree with Terri. I also have an 1850 and will use it until parts are no longer available which could be,. like she said, years. We also have a local company do the PM and repairs and will use it until it absolutely cannot be repaired. Colleen Forster HT(ASCP)QIHC University of Minnesota BLS

Re: [Histonet] GSH Virtual Summit- THIS SATURDAY

I'd love to join you but I will be working that entire day Colleen Forster On Wed, May 19, 2021 at 3:24 PM Lauren Sweeney via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi Everyone, > > It is not too late to join us for the GSH Virtual Summit on May 22, 2021 >

Re: [Histonet] Animal Histology

Wow, what an excellent list John. I didn't ask the question but will surely use the response. Thank you for sharing. Respectfully, Colleen Forster HT(ASCP)QIHC University of Minnesota On Mon, May 3, 2021 at 12:32 PM John Kiernan via Histonet < histonet@lists.utsouthwestern.edu>

Re: [Histonet] Is there a way to orient FFPE sections with an indicator that remains visible post staining?

t;map" and then that goes with the block. It works well. Colleen Forster On Thu, Apr 8, 2021 at 12:30 PM Cheryl via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi everyone- > We have a researcher who needs to put multiple samples in one block for > quality and cost control.

[Histonet] IHC with zamboni's fixed tissues

Hello Histonet, Can you give me feedback on trying to do IHC on samples fixed in Zamboni's fixative? I have an idea but need confirmation . Thank you in advance Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612

Re: [Histonet] Water under sections

, Colleen Forster On Mon, Jan 25, 2021 at 11:26 AM Tyrone Genade via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello, > > > On Saturday, January 23, 2021 1:00 PM, < > histonet-requ...@lists.utsouthwestern.edu> wrote: > > > In my lab, water und

Re: [Histonet] MelanA

kit back into 4 degrees. Just one thought. Colleen Forster HT(ASCP)QIHC U of MN On Thu, Oct 22, 2020 at 2:30 PM Rhonda McCormick via Histonet < histonet@lists.utsouthwestern.edu> wrote: > I thought I'd reach out and see if anyone can help with some IHC > troubleshooting (or know

[Histonet] Tissue processor advice

have used them can share. Thank you in advance. Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 612-626-1930 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman

Re: [Histonet] Humidity in the lab

Totally following this one... And what do you use to record humidity and what would be the cut off for good staining , especially for IHC stains. Colleen Forster On Wed, Aug 19, 2020 at 6:30 PM Patti Nelson via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello Histo pe

Re: [Histonet] Need a procedure

info/histonet > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Questions on how to operate a ThermoFisher Lab Vision autostainer 3602D

The Biocare certain was called the Nemesis. They are phasibg them out but might be willing to come assist you. Colleen Forster On Thu, Oct 24, 2019, 2:43 PM Eileen Akemi Allison via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Ask Biocare or Dako. Unbeknownst to most of t

Re: [Histonet] Fwd: [HISTONET] Biocare

out of business. Can anyone confirm > or deny this? > > Thanks. > Sent using the mail.com > mail app > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet &

Re: [Histonet] Mallory-Azan stain

Wow John, Great information.,always so much to learn!~ Thank you, Colleen Forster U of MN On Tue, Sep 10, 2019 at 10:55 AM Morken, Timothy via Histonet < histonet@lists.utsouthwestern.edu> wrote: > John, we love it when you "ramble!" It gives us an appreciatio

Re: [Histonet] Tissue scrolling

results. Respectfully, Colleen Forster HT(ASCP) U of MN On Sun, Sep 8, 2019, 10:36 AM John Garratt via Histonet < histonet@lists.utsouthwestern.edu> wrote: > The best I can offer is to advise you to wipe the knife holder area down > with a cleaning solvent and use a fresh blade. C

Re: [Histonet] tissue blocks stored in cooler C for 2wks - any successwith IHC?

I agree with Brett. I believe the histological aspect will be horrible and probably not worth the time and money spent. I feel you would be terribly disappointed with the lack of usable results. Colleen Forster HT(ASCP)QIHC On Thu, Aug 22, 2019 at 11:14 AM Brett Connolly via Histonet < histo

Re: [Histonet] IHC mouse optomization

Biocare Medical Promark series are also great animal polymer based detection that work very well. https://biocare.net/products/detection/ Colleen Forster HT(ASCP)WQIUC U of MN On Wed, Jun 12, 2019 at 10:42 AM Paula Keene Pierce via Histonet < histonet@lists.utsouthwestern.edu> wrote: &g

Re: [Histonet] CK Pools

utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 *If submitting histology request please also forward to Lori Holm at ho...@umn.edu *

Re: [Histonet] ER/PR controls

__ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 *

Re: [Histonet] [EXTERNAL] Re: New York Qualifications for Histology Supervisor

Anatomic Pathology Manager * > > (914) 302-8424 > *ap...@cmmedical.com* > > > > *From:* Colleen Forster [mailto:cfors...@umn.edu] > *Sent:* Thursday, February 21, 2019 11:59 AM > *To:* Perl , Alison > *Cc:* Melissa Owens; histonet@lists.utsouthwestern.edu >

Re: [Histonet] New York Qualifications for Histology Supervisor

Wow...this is just crazy~ The regulations leave histology in a real tough spot... Feeling for all of you and yes, for the future histology techs coming up~ Colleen Forster U of MN On Thu, Feb 21, 2019 at 10:52 AM Perl , Alison via Histonet < histonet@lists.utsouthwestern.edu> wrote:

Re: [Histonet] Words, phrases and names in histotechnology. A free glossary.

Yes! Your expertise is such a valuable resource.. C On Wednesday, February 13, 2019, Bob Richmond wrote: > You're welcome! > > I sat down and read through most of it as soon as I saw it. So glad John > Kiernan's back! > > > On Wed, Feb 13, 2019 at 8:48 PM C

Re: [Histonet] Words, phrases and names in histotechnology. A free glossary.

THis looks likje a great resource...I will take time to look it over. Thank you for all your valuable mentorship over the years Respectfully, Colleen Forster U of MN On Wed, Feb 13, 2019 at 4:35 PM Bob Richmond via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Joh

Re: [Histonet] guinea pig IHC

; Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 *If submitting histology request please also forward to Lori

Re: [Histonet] Histonet - IHC Manual staining

Hello Dave, I still do a lot of manual work and I also use Biocare reagents. They work well and their customer service is very good. Colleen Forster On Fri, Jan 18, 2019 at 12:58 PM Jeanine Ronkowski via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi Dave:Our lab has used

Re: [Histonet] IHC Stainer

. Colleen Forster HT(ASCP)QIHC U of MN On Thu, Aug 30, 2018 at 12:58 PM, Jennifer Kempf via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Biocare Medical Intellipath...its completely open and is a continuous load > machine, meaning you can start a run on one rack while still loadin

Re: [Histonet] HT Position in Alaska

This sounds wonderful..I wish I were in a position to swoop it up! Colleen Forster HT(ASCP)QIHC On Wed, Jun 6, 2018 at 9:24 AM, Arrington, Karla A via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello All, > > I hope everyone is enjoying the early part of summer. &

Re: [Histonet] Leica

 On Tue, May 1, 2018 at 12:07 PM, shultz11 via Histonet < histonet@lists.utsouthwestern.edu> wrote: > > Leica equipment is now being made in China not Germany. I wish I would > have known this before purchasing equipment. > Kendra ShultzSent from my Verizon, Samsung Galaxy smartphone >

Re: [Histonet] Radiation sterilization and IHC

To do samples that you suspect prion disease also needs to be done in a lab specifically designed for that. I agree, formalin is sufficient., Colleen On Mon, Apr 23, 2018 at 11:14 AM, Rene J Buesa via Histonet < histonet@lists.utsouthwestern.edu> wrote: > I believe that is an extreme and

Re: [Histonet] Stain for specific nerve types in sacral spinal cord

Following...would like to see the answer. Colleen Forster On Tue, Mar 27, 2018 at 3:25 PM, Karl Koessler via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Does anyone know of a stain or marker I can use to differentiate > sympathetic from parasympathetic in sacral spinal c

Re: [Histonet] IHC validation

I agree Terry, The TMA slide is a very economical and powerful way to validate with minimal slides needed. Colleen Forster On Tue, Mar 20, 2018 at 12:54 PM, Terri Braud via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Just another note: You can order unstained tissue mi

[Histonet] atlas for Drosphilia fly

? Thank you in advance!~ Respectfully, Colleen Forster U of MN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Resource for setting up a hard tissue lab

you in advance! Respectfully and hopeful, Colleen Forster U of MN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] processing Dosophilla flies

Can anyone give me a processing schedule for Drosophila flies...very tiny. Thank you in advance! Colleen Forster HT(ASCP)QIHC U of MN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Reference Lab in So. CAL

to work with them.. Respectfully, Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory University of Minnesota 612-626-1930 On Thu, Jan 11, 2018 at 3:27 PM, Jennifer MacDonald via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Thank you to all that responded. I have a li

Re: [Histonet] Beta-Amyloid antibody for IHC

ichard* > > > > *From:* Colleen Forster [mailto:cfors...@umn.edu] > *Sent:* Wednesday, December 27, 2017 4:23 PM > *To:* Cartun, Richard > *Cc:* histonet@lists.utsouthwestern.edu > *Subject:* Re: [Histonet] Beta-Amyloid antibody for IHC > > > > CAUTION: This ema

Re: [Histonet] Beta-Amyloid antibody for IHC

Do you knot want to use 6E10 anymore? I believe you can still get it from Biolegends? https://www.biolegend.com/en-us/products/purified-anti-beta-amyloid--1-16-antibody-11228 Respectfully, Colleen Forster HT(ASCP)QIHC *University of Minnesota* On Wed, Dec 27, 2017 at 3:20 PM, Cartun, Richard

Re: [Histonet] Mouse bladder

gt; > > On Tuesday, December 19, 2017 9:45 AM, Colleen Forster via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > Be sure you are processing them on an overnight process. Even if the > samples are small it takes that time to process the agar properly. > >

Re: [Histonet] Mouse bladder

ould think agar might be the same. Just a thought, Colleen Forster HT(ASCP)QIHC U of MN On Tue, Dec 19, 2017 at 11:29 AM, Liz Chlipala via Histonet < histonet@lists.utsouthwestern.edu> wrote: > I would try histogel instead of agar. > > Liz > > Elizabeth A. Chlipala, BS, HTL

Re: [Histonet] Translational science/medicine/research IHC lab question

I am following, would love to see the responses. Colleen Forster On Thu, Sep 28, 2017 at 5:14 PM, Judi Ford via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi Everyone, > I am interested in connecting with techs/managers who are working in or > have developed a tran

Re: [Histonet] Response Fixation and IHC for animal tissue

Cristi, I follow the general guidelines of good fixation for the msajority. IF I am testing something that has specific in the clinicla lab, I try it the same on animal tissues for the same thing. Colleen Forster HT(ASCP)QIHC On Thu, Jun 15, 2017 at 4:05 PM, Eddie Martin via Histonet

Re: [Histonet] Butterfly histology

Following. Colleen Forster On Mon, May 29, 2017 at 10:04 AM, Gregoire, Rhonda (AGR) via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Does anyone have any experience processing and paraffin embedding > butterflies? > > Rhonda Gregoire, MLT > Superviso

Re: [Histonet] Kawamoto protocol online from Rowe lab

Thanks Gaylethis is the system the pathologist wanted to but$3000.00 and he had no money I want to try and work with more reasonably priced ideas first.not sure I will ever get this one downI think Ill have better luck with bugs! I will watch this video and read the notes

Re: [Histonet] CV Path Institute IHC-HT Job opportunity

This sounds like a wonderful opportunity.I wish I were in a position to go for it! C On Mon, Aug 15, 2016 at 12:43 PM, Lila Adams via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Good afternoon everyone, > I thought I would post this job opportunity. > CV Path Institute Job

Re: [Histonet] JOH journal collection

Brett, Are you interested in sending all mof them as a complete (-7 issues) set? Colleen Forster U of MN On Tue, Aug 9, 2016 at 8:57 AM, Connolly, Brett M via Histonet < histonet@lists.utsouthwestern.edu> wrote: > HI all, > > Is anyone interested in old issues of JOH? My c

Re: [Histonet] CD3 antibody for FFPE mouse tissue

it at 1:400, citrate retrieval, 1 hour primary incubation. I use the Rabbit on Rodent polymer from Biocare. Respectfully, ' Colleen Forster On Tue, Jun 21, 2016 at 10:53 AM, Connolly, Brett M via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Liz, > As long as it detects mouse

Re: [Histonet] Shrinkage

I find the same as Caroline Miller, about 20-25% with formalin fixation and routine processing. C On Mon, Feb 22, 2016 at 3:41 PM, Caroline Miller via Histonet < histonet@lists.utsouthwestern.edu> wrote: > hi All, I have done some experiments in this area for mouse brains, and I > find that

Re: [Histonet] Missouri/Arkansas Joint Meeting May 19th to 21st, 2016 Branson MO

This looks wonderful! Be sure to keep me on your list Pam so I can consider this meeting! C On Thu, Jan 28, 2016 at 2:50 PM, Pamela Marcum via Histonet < histonet@lists.utsouthwestern.edu> wrote: > > > Good Afternoon, > > > > > We would like to invite all of you to Branson Missouri for the

Re: [Histonet] NHPD

I think that is about average for good Tshirts these days. C On Tue, Jan 26, 2016 at 2:43 PM, Bitting, Angela K. via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Is it me or does anyone else think $20 is a lot of money for a T-shirt? > The Histotechnology Professionals Day T-shirts on

Re: [Histonet] CD8 IHC on FFPE mouse tissue -what your experience?

I am interested to see what you hear BrettI need to do these same markers and am not having good luck in FFPEfrozens , great...FFPE NOT! C On Tue, Nov 3, 2015 at 7:32 AM, Connolly, Brett M via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi all, > I have a project that will be

Re: [Histonet] Help: IHC service

I'd be very interested in this as well...could you shaere Karen. Thanks. C On Mon, Nov 2, 2015 at 11:43 AM, Karen Cai via Histonet < histonet@lists.utsouthwestern.edu> wrote: > HELP: > > > > Hi, > > Is there anybody can provide me the price list/structure of the custom IHC > services? > > >

Re: [Histonet] Peggy Wenk's passing

So very sorry to hear of Peggy's passing. She was such as inspiration to us all. She will be greatly missed. May God grant her family the peace and grace to pass into the following days with strength. She will now be soaring with the eagles watching over everyone. Rest in peace Peggy, Colleen

Re: [Histonet] IHC-start-up

I totally agree with William. I do animal IHC and I am, using the Biocare Nemesis, which be the same as the Dako platform. Completely open so you can do what you need to at a cost you can afford Colleen Forster HT(ASCP)QIHC u of MN On 1/23/2014 11:44 AM, Will Chappell wrote: Doing ihc

[Histonet] unsubscribe for NSH

___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Unregistered HT

Exactly Jennifer! C On 9/10/2013 6:32 PM, Jennifer MacDonald wrote: As long as we do not need certification, licensure and minium education requirements we will not be recognized as Laboratory Professionals. From: Marcum, Pamela A pamar...@uams.edu To: 'joelle weaver'

[Histonet] IHC after EDTA decalcifying

and in the human tonsil the stain is great. Any suggestions.. Thanks in advance! Colleen Forster U of MN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] HE staining question

I am interested in the answer as I use this same technique on my rodent samples and have not had this issue. On 7/2/2013 8:07 AM, Connolly, Brett M wro Histonetters - We had some rat tissues that were removed at necropsy and immediately fixed in formalin for 48 hrs. Since the tissues

[Histonet] CD200(OX2) that works in FFPE mouse samples

To all the histonetters, IS anyone doing CD200 staining in FFPE mouse samples with good results? If so, please share your information. Thanks in advance...your help is always appreciated!! Colleen Forster U of MN ___ Histonet mailing list

[Histonet] BrDU

What are you guys using as your primary for BrDU these days? Thanks! Colleen Forster U of MN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Stain to ID eosinophils specifically

Does anyone have access to this reference for me? I have a person looking to ID eosinophils in fat samples. I am looking for a stain that will make finding them easierthis reference was mentionedany other suggestions? These are FFPE samples. Suggested Ref ex. LIllie 4th Ed. pp750.

[Histonet] zebra fish experts......

!! Colleen Forster U of MN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] EGFR primary

Hello everyone, Can you please share with me the EGFR primary antibody you use and what pretreatment you use with itthanks in advance!! Colleen Forster ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu

Re: [Histonet] CD11b

Me too!!! C On 11/6/2012 3:44 PM, Bell, Pat wrote: Hello to Everyone!! :) Has anyone worked up CD11b in human tissue? We have tried one from Novus and one from Serotec with no staining or staining that was not specific. I would appreciate any help anyone could give! :) Thank you, Pat Pat

Re: [Histonet] RE:Changing dynamics in histotechnology (Jesus Ellin)

Well said Toysha. I don't think they have even really incorporated IHC into the histology programs and that IS a routine technique now. We cannot promote in a field what we do not teach! Colleen Forster HT(ASCP)QIHC U of MN On 9/18/2012 12:06 PM, Mayer,Toysha N wrote: Being in HTL

Re: [Histonet] endothelial cell marker

I second Patsy! I have used this antibody with both AP and DAB and have had excellent results. Colleen Forster U of MN On 9/13/2012 2:05 PM, Patsy Ruegg wrote: By far the best ab for endothelial cells in mouse tissue is rat anti CD31 from Dianovo, it picks up the really early forming

Re: [Histonet] RE: Slide printing systems

Try Dako, they have a nice little slide printer out there now. Colleen Forster U of MN On 8/15/2012 10:25 AM, Bell, Pat wrote: I also have the slide-mate and have a lot of problems with the printing. We also have to spend a lot of time rewriting and labeling them. I have been told

[Histonet] written procedures

processor. I am interested in procedures with and without the use of a hydrometer. Thanks in advance!! Colleen Forster U of MN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Ki67 antibody to stain Mouse tissue

in human gut and yes, I have done it on most major organs of the mouse. Colleen Forster U of MN On 8/8/2012 10:38 AM, Eva Permaul wrote: Just to clarify. Are you using it on mouse tissues? I am only asking because the Thermo Scientific/Labvision/Neomarkers data sheet says it has only been

Re: [Histonet] Ki67 antibody to stain Mouse tissue

Thanks Jan, that would be the same one I have and it works great! Colleen Forster U of MN On 8/6/2012 2:59 PM, Jan Shivers wrote: We have had success with Lab Vision's rabbit polyclonal on mouse tissue (catalog # RB-9043), utilizing HIER for antigen retrieval. Jan Shivers U of MN Vet Diag

Re: [Histonet] Ki67 antibody to stain Mouse tissue

do this it will save you a LOT of headache. Colleen Forster U of MN On 8/6/2012 3:57 PM, Colleen Forster wrote: Thanks Jan, that would be the same one I have and it works great! Colleen Forster U of MN On 8/6/2012 2:59 PM, Jan Shivers wrote: We have had success with Lab Vision's rabbit

[Histonet] TMA blocks

of them into one big block? Thanks in advance!! Colleen Forster Bionet Histology Laboratory U of MN, AHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Need feed back from any users out there!

Hello Histoland, Can anyone who may have used or is using this piece of equipment give me their feed back? We are considering it to do an online dewaxing and retrievel with the Biocare Nemesis stainer. Thanks in advacne for your comments! Colleen Forster HT(ASCP)QIHC U of MN Lab

[Histonet] Pearls Prussian blue with green counterstain

I am wondering if any of you have ever used a Fast Green counter stain in place of the Nuclear Fast Red? If so, what timing did you use? Thanks, Colleen Forster U of MN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] CD31 for rat tissue

Hello Histonetters, I am looking for a CD31 that has been proven to work well in rat tissue samples...have any of you out there done this with success? I have good human and mouse antibodies not rat! Thanks in advance! Colleen Forster Bionet Histology Research Laboratory U of MN 612-626

[Histonet] In need of a Methods in Cell Biology chapter

for the autoradiography of diffusible compounds by W.E. Stumpf. This volume is from 1976 so it goes way back!! HELP!! anyone. Our medical library does not have this book and to get it on Amazon was $172, too steep for my pocket. Thanks Colleen Forster HT(ASCP)QIHC U of MN

[Histonet] Cyclin D2 staining

Has anyone out in histo and been able to get good Cyclin D2 staining in FFPE samples? If so, please share. Thanks, Colleen Forster U of MN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo

Re: [Histonet] Slide labeler

And if you have their coverslipper, it doesn't like the rounded edges either!! Colleen Forster. Cheryl Crowder wrote: Igor - We have a Leica slide labeler. It is easy to use, but we have found that you should use slides with 90 degree corners and a rough surface. Slides with clipped

[Histonet] To my fellow histotechs....

* MUM-1 Thanks in advance... Colleen Forster HT(ASCP)QIHC Bionet Histology Service U of MN 612-626-1930 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] CD31 working protocol

Biocare has a great CD31 for mouse and rat too. You do need the kit and their primary but you can use formalin fixed samples with great results! Colleen Forster U of MN Amy Porter wrote: BD Biosciences has a great rodent CD31 for mouse and rat - however you need to use their Zinc Fixative

Re: [Histonet] What percent of HTL's do not have a BS degree?

To Ron Martinhistology IS BOTH A PROFESSION AND A CAREER! You need a new job! Colleen Forster HT(ASCP)QIHC Wahlberg, Nikki wrote: I would just like to add that in my opinion it is people who make statements like the one below that are holding our field back from being seen

Re: [Histonet] Re: IHC on frozens

I disagree. I do heat retrieval on frozen sections that have been fixed in a formalin fix. If you use the correct protocol you can get very nice IHC on these. Colleen Forster HT(ASCP)QIHC Anatomic Pathology Research Laboratory U of MN Johnson, Teri wrote: In response to this thread

Re: [Histonet] CNS Fixation

Animal brain is the same. IF you aior dry overnight and then into the oven before starting your stains you will not lose sections. Colleen Forster U of MN Barbara Albert wrote: We've found this also. Even 30-60 minutes air drying helps alot with human brain. We use regular slides and NBF

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