Re: [Histonet] vasculature staining in 3D

Hi, I have done retinal flat mounts to visualize vasculature with lectins and used chromagenic instead of fluoro. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing

Re: [Histonet] Histonet Digest, Vol 243, Issue 6

When I need to handwrite on slides or cassettes, I use Sakura Pigma Micron archival ink pens. They come in different tip sizes and colors and are found at any office supply store. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH

Re: [Histonet] Microcredentials for Histology

Here! Here! I sure would not want the pilot flying a plane I was on to have micro credentials. Like, hey I learned how to raise the landing gear this week.  Standards should be raised, not doing just enough to get by. In histology, all steps in totality create the final product. I still think

Re: [Histonet] Fixing and processing mouse eyeballs

Hi, I have done 10s of thousands of mice eyes, as well as other species, processed to paraffin in my career with no retinal detachment and enhanced IHC and ISH, but using my own method. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK

Re: [Histonet] Small Inexpensive Stainer for Special Stains

Just a thought, but H stainers have multiple programs to code. Having a second machine to use for specials would be an option. Maybe even bring slides to water and use different rows of containers for different stains. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N

Re: [Histonet] Alcian blue van gieson double stain

Hi, if they are wanting to stain elastic, Alcian blue, and Van Gieson, I recommend doing the Verhoff elastic stain first, then the Alcian blue, and then the Van Gieson. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH

Re: [Histonet] Goat Antibodies

Hi, Vector Labs has Goat polymer ImmPress detection kits in both alkaline phosphatase and HRP. I find any polymer detection greatly reduces background. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH

Re: [Histonet] IF with permanent mounting media?

Hi, you can use "Crystal Mount" first, let it dry, and then coverslip with xylene/resin. I do this routinely with AEC IHC and oil red O and other fat stains. Crystal Mount only on retinal flat mounts to be shipped out to others for future staining, as it can be removed with water, FYI: Crystal

Re: [Histonet] Tissue Orientation Markers

You could use pieces of fixed/processed plant leaf embedded on edge. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb

Re: [Histonet] Antibody diluent

I just purchased antibody diluent from Electron Microscopy Sciences. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb

Re: [Histonet] Old Tissue Tek processor

There is one for sale on ebay. https://www.ebay.com/itm/FISHER-HISTOMATIC-TISSUE-PROCESSOR-MODEL-166-MP/151784782912?hash=item235713fc40:g:CbkAAOSwgQ9V02Xl Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH

Re: [Histonet] Repairing a damaged TMA block

Hi, align the block as well as you can in its corresponding size mold and place in a 37C oven for about 30 minutes to an hour. This will soften the block enough that you can use a straight edge to gently realign the warped side. You may need to repeat a couple of times to not force it. Paula

Re: [Histonet] Update info. on old topic?

I am interested in this too, with my main question being the quality of digital images from film coverslipped slides. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is

Re: [Histonet] Crystals forming in Hematoxylin

That is the aluminum salt precipitating out. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Thursday, July 9,

Re: [Histonet] Anyone having difficulty with tissue adhesion?

Hello, 99% of my work are eyes and they are notorious for not adhering well. One thing I have found is that white glass slides work the best and cannot use any green glass. I would like to try your slides. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake

Re: [Histonet] Shandon-Cryotome-Fse battery change ?

Yes, it is the same as the battery on a computer tower motherboard to keep the clock going. Turn off. Unplug. Remove panel. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife

Re: [Histonet] UV Light in Fume Hood

It will need to be UVC or UVD. UV light works by scrabbling the DNA in bacteria, viruses, and mold. But I will also scrabble yours too. Hospitals, airports, and other businesses are starting to use UVD robots to disinfect patient rooms and large areas with no people present at the time. UV light

Re: [Histonet] IHC DAB

Since you are newbies, I am just putting IHC 101 out there.  Permanent red is for alkaline phosphatase detection and DAB is for HRP. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp

Re: [Histonet] Histonet Digest, Vol 197, Issue 23 recovering RNA from FFPE

Biochain too. Paula On Saturday, April 25, 2020, 01:08:53 PM CDT, Steve McClain via Histonet wrote: 1) Does anyone know of a method of recovering RNA from FFPE? 2) Does anyone know a lab that has done it? Thanks, Steve A. McClain, MD McClain Labs 45 Manor Road Smithtown, NY 11787

Re: [Histonet] Histonet Digest, Vol 197, Issue 23 recovering RNA from FFPE

By doing an internet search for FFPE PCR Platform, Thermo, Agilent, Biogen, Qiagen, etc. all have this. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a

Re: [Histonet] Recommended thickness of Amyloid sections

As this is my 41 year of being a registered HT, I was taught that slides for amyloid are to be cut at 8-10µm. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing

Re: [Histonet] Metal Testing

A lab with X-Ray diffraction on an SEM could help. Try the university electron microscopy labs. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp

Re: [Histonet] EM as a "contract lab" lab for clia purposes

Yes, they would still need the certification, however as only the technical part. A pathologist will need to be designated the medical director and all pathologists reading the results will need to have their credentials added. The medical director will need to also sign off on any complex

Re: [Histonet] Cutting room and social distancing

Yes, that is one of the CDCs' recommendations. Use different shifts to spread out people. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. -

Re: [Histonet] hard Tick histology

The exoskeletons of insects are made of chitin and contain little calcium. Lysol spray contains a type of phenol which will soften chitin. The phenol is what kills bacteria, mold, and viruses by destroying their outer shells. Try spraying Lysol in a small container so you have a few mls. It will

Re: [Histonet] alternative labels for zebra printer TLP 2742 connected to dako agilent autostainer plus

Hello, I purchase my solvent resistant labels and thermal ribbons for my Zebra printer from Electronic Imaging Materials. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is

Re: [Histonet] X-gal staining

I have done paraffin processing and sectioning of X Gal stained eyes and embryo heads. The staining is done for the blue color first, post fixed, and then processed.Xylene should be avoided, as it is what removes the X Gal target, and a xylene substitute used such as StatLab's XS3.

Re: [Histonet] How do you section PPFE tissue in embedding rings?

Hi, I used embedding rings back in the 80s and still use the chuck adapter for large blocks. I do eyes. I have a couple John's Hopkins chuck adapters and could loan you one. Send me a picture of your microtome with a closeup of the chuck adapter to see if it will fit. Do you have an old AO 820

Re: [Histonet] IHC mouse optomization

Vector Labs ImmPress kits rock for animal IHC. https://vectorlabs.com/browse/immpress-polymer-detection-kits-for-ihc Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953www.excaliburpathology.com On Wednesday, June

Re: [Histonet] Looking for vendor for Zeus Wash solution

You can also use Michel's buffer. Electron Microscopy Sciences has it. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953www.excaliburpathology.com On Thursday, March 21, 2019, 12:29:45 PM CDT, Martha Ward-Pathology via

Re: [Histonet] Roche "bashing"

Yep. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953www.excaliburpathology.com On Wednesday, March 6, 2019, 9:14:39 PM CST, P Sicurello via Histonet wrote: It's not Roche "bashing", these are messages come

Re: [Histonet] benchtop fume hoods

I use a Labconco. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From: Allan Wang via Histonet To: Cc:

Re: [Histonet] Backup Power Supply

Hi,  this is one that can charge by being plugged in and is larger than ones for computers. http://www.wisefoodstorage.com/humless-portable-power-storage-and-fuelless-generator.html They also have larger ones. I have no relationship with Wise, except being a customer. Paula Keene Pierce, BS,

Re: [Histonet] Need help identifying a product!!!

Have you tried MarketLab? They have things like that. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From: "Cooper, Brian via Histonet"

[Histonet] Butterfly Histology

Hi, I do drosophila and other insects. Use an alcoholic fixative such as Davidson's for better infiltration. Transfer to 70% EtOH after 24-48 hours. Normal processing schedule with 30 minutes to an hour starting at 70-80%. 1-1.5 hours each for paraffin. Paula Keene Pierce, BS,

Re: [Histonet] No Ribbon

Hi, the blades are packed with a tiny, bit of oil. This can cause you to not obtain a ribbon. Try gently cleaning with xylene, followed by alcohol before sectioning. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX

Re: [Histonet] Tissue Fixation

Bravo Rene! Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From: Rene J Buesa via Histonet To: T H ;

Re: [Histonet] malarial pigment

Lee Luna's third edition. Page 43, Method II 1. Deparaffinize and hydrate to water.2. Wash in distilled water.3. Immerse slides in 100 ml of 70% alcohol, to which has been added 2 ml of 28% ammonium hydroxide, for 3 hours.4. Rinse in water.5. Rinse in 1% glacial acetic acid.6. Wash well in

Re: [Histonet] aniline oil/Holzer stain

You could try adding aqueous NaOH slowly to what you have until you start getting tiny white droplets in the water. Then they will fall and form an oily layer below the water. Decant the water or use a separator funnel to drain the aniline oil out the bottom.  Paula Keene Pierce, BS,

[Histonet] Pan Cytokeratin

Hello, would anyone be willing to donate a recently expired, about to be tossed, vial of pan cytokeratin for a research project? I will pay shipping. Thank you, Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX

Re: [Histonet] Frosted slides

Hi, they are called Dakin slides.  A quick search found:  http://www.emsdiasum.com/microscopy/products/preparation/slides.aspx  Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com

Re: [Histonet] Shandon Varistain Gemini Slide Stainer

This is so true! I had a Fisher Histomatic automated slide stainer that was manufactured in 1985 that I used until the building took a direct lightning strike JUST LAST YEAR! My old VIP is still going!  Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake

Re: [Histonet] floor vibration

Refuse the space and ask to be moved somewhere else. Years ago our EM scopes were on the fourth floor and lines in photos taken could be seen from the vibration from people simply walking down the halls. Finally moved the scopes to the basement on a section of concrete cut out completely

[Histonet] Job Opening in CA

| | | | | | Dear Friends & Family,I am seeking a Research Scientist I, Stem Cell Biology  for a stem cell therapy project that we just started. The funding has been  secured for the entire project. The position is available immediately and the description is shown below. If you know