Hey Charles,
I am sure you have thought about this but your retrieval process could be too
stringent if you are having over expression.
Thanks,
Timothy Higgins, HT(ASCP), QIHC(ASCP)cm
Histology Supervisor
Center for Disease Detection/LabCorp
tim.higg...@cddmedical.com
Message: 6
Date: Wed
Hey Charles,
I have to agree with Rene. They are either being left out after sampling prior
to fixation or possibly coming into contact with air during the processing run.
Make sure your solution are covering all the cassettes, don't let them float
free inside the processing chamber.
Thanks,
Cap states, "should test a minimum of 10 positive and 10 negative tissues".
Does not state "cases" but "tissues", so we used a block for positive and
negative from the same case.
Thanks,
Tim
-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu
[mailto:histonet-requ...@
I check pH every time I make a new batch(bottle) and then periodically there
after.
Timothy N. Higgins, HT (ASCP), QIHC
> Message: 10
> Date: Tue, 11 Feb 2014 16:23:22 +
> From: "Gupta, Nilesh"
> Subject: [Histonet] Buffer pH monitoring
> To: "'histonet@lists.utsouthwestern.edu'"
>
They come in, give their opinions on how to make your workflow better. I
didn't get much from it, we didn't make any changes, more of a selling tool in
my opion. I guess if your workflow was terrible, they might be able to offer
good advice.
On a positive, they were all very nice.
Thank,
T
I have used orange peel for years. I never had a problem with the pathologist
not approving the control for use on patient stains. Its "naturally
occurring", a petri dish would not be naturally occurring in my mind.
Once I verified the orange peel does stain the fungus the same way it does
Hey Mary,
I would suggest observing your
stainer to ensure the proper drop zones are being performed and not just
dropping closest to the label. I used Dako for years and loved them but you do
have to make sure it dispenses properly from time to time. They use drop zones
unlike Ventana which use
Hello Annieinarabia,
You are quite the advocate for Sir Rene J. of the most respected voices on this
forum.
Truly if you want to be respected, give respect and do not degrade other as he
does from time to time (in my opinion). Give substance in your responses and
not comment using some pomp
Hey Martha,
You have to forgive Rene J. she speaks sometimes and has nothing good to say
but keeps speaking. There is never a bad question, if you don't have a good
answer then keep it to yourself as I usually do.
"Adequate forum". really? I guess no one on Histonet is allowed to share th
Hello All,
Have you ever had a Pathologist say, if you have one tissue control (placed on
the same slide as the patient tissue) per antibody, per patient case, but there
are multiple blocks (same tissue type) from the same patient case, that would
be batch controlling?
Hope that made sens
Hey Kathy,
It is not considered a High Complexity test on the staining side of it. You
don't need any special degree or certification, I know we all want our
profession to fetch more money and respect and a good way is make a mountain
out of molehill to use an old saying.
Now here is th
I need some MOHS procedure help, anyone want to help me offline? I would
really appreciate it!!
Thanks,
Tim
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All I have to go by is CAP's response to the question, "Is IHC a High
Complexity Test". Their verbal answer is no, but there is nothing formally
documented. The only documented statement is from 2010 Webinar during the
Question and Answer portion. It is as follows,
Laboratory Accreditation
The professional interpretation is considered a high complexity test but not
the actual technical component.
Thanks,
Timothy N. Higgins, HT (ASCP), QIHC
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offered for the benefit of all, and especially for the benefit of those
posting a problem.
At least that is what I always try to do!
René J.
From: "joellewea...@hotmail.com"
To: Tim Higgins ; histonet@lists.utsouthwestern.edu
Sent: Thursday, January 10, 2013 11:07 AM
Subject: Re:
Gale and Pam, people on Histonet love to sit on their soap box and spew out
ridiculous statements without any thought behind them at times.
To say you need to "scale the volume down to a point where it is manageable"
(tell that to your boss!! Whatever!!) is coming from someone who obvio
Hey Deloris,
I would have the dispensing nozzles on the XT checked, could be it is not
getting rinsed properly. Had that issue with my Ultra not to long ago. No
warnings or errors showed up, just a clogged nozzle tip.
Tim
-Original Message-
From: histonet-boun...@lists.utsouthweste
Nobody has mentioned it, but can you imagine the amount of revenue the
manufactures would loose if they came out and supported the decision on not
using negative controls. That’s not going to happen!!
I have used both systems the Dako and the Ventana (Benchmark, Benchmark XT,
Ultra) and both
All great points, awesome information, that’s why we all enjoy histonet.
One thing I've learned over the 20 plus years in histology is every person
is different and you cant stick them all in the same box. I personally
don't believe 40 blocks an hour is unreasonable as an average as long as yo
Hey Dorothy,
It's 10 years for CAP, but you need to check with your state as well. I
know for New York you must hold them for 20 years.
Thanks,
Tim
Message: 20
Date: Tue, 11 Sep 2012 08:09:24 -0700 (PDT)
From: Dorothy Glass
Subject: [Histonet] blocks and slides
To: histonet@lists.utsouthwe
Agree, only if the antigen has been bound by a fixative.
-Original
From: Daniela Bodemer
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, July 4, 2012 7:02 AM
Subject: [Histonet] Frozens and antigen retrieval
Hi all,
A question from my student: antige
Nuclear Fast Red needs to be heated during prep and it does take a little
while to dissolve, it never really dissolves entirely, probably saturates
the solution pretty fast.
Tim
Message: 1
Date: Tue, 29 Nov 2011 11:29:07 -0700
From: "Patsy Ruegg"
Subject: [Histonet] nuclear fast red
To:
Message
Hello Histonetters,
Anyone have a spirochete control block(s) they would like to trade?
Thanks,
Tim
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Hey Jessica,
We have two Histostar's that have been here since March and have not had any
issues with them. Can't say anything bad about them, yet.
Thanks,
Tim
Subject: [Histonet] Histostar Embedding Station
To: "Histonet@lists.utsouthwestern.edu"
Message-ID:
<1319563285.61798.yahoomail...@w
Hey Histonetters,
Does anyone use the p57 (kip 2) antibody from Thermo (Lab Vision)? I am still
having trouble with the pathologist saying it's not staining the
syacitiotrophoblasts. Any help with a protocol or even me being able to send
in a slide that you could stain and send back to be wou
I have been in the histology field for 20 years and never heard of having to
remove the labels prior to disposal through an approved vendor. I don't
believe that the labels from blood tubes are removed prior to disposal, this
would be the same type of scenario.
Thanks,
Tim
Message: 9
Date: Mon
Does anyone use the p57 (Ab3) concentrated antibody from Lab Vision (Fisher)
for Molar pregnancies on the Ventana platform using the UltraView detection
system? What is the protocol and dilution you run?
Any help would be greatly appreciated!!
Thanks,
Timothy Higgins, HT(ASCP), QIHC
Histolo
I have worked at labs that reuse molds and ones that clean between uses.
Guess it all depends on what your definition of a "clean" mold is. If you
have the time and space, not reusing the molds is best practice but not
always practical.
Thanks,
Tim
Message: 10
Date: Tue, 20 Sep 2011 09:44:26 -
Good instument, been working with it for about 6 months, using the Fast Flex
system for rapid tissue processing. What did you want to know specifically?
Tim
Message: 8
Date: Tue, 13 Sep 2011 09:11:53 -0700
From: Davide Costanzo
Subject: [Histonet] STP420
To: histonet@lists.utsouthwestern.edu
Me
Hello Histo Friends,
Has anyone had to validate IHC antibodies when you do not have tissue processed
in house to use for your validation. I do have control tissue that stains
properly but it was acquired from outsides sources with verified proper
predicted staining.
If you have done this type
How would the expression, "you need experience to get the job, and you need
the job to get experience" work if a person went through flight school to be
a pilot but did not receive any actual flight training but was then allowed
to get a job as a pilot flying a commuter plane in charge of peoples l
I have to agree with the fact that students should not be released into the
workforce without any practical experience. We team up with a local college
(St. Phillips College, San Antonio TX) that produces several students a year
and we along with other facilities help in the rotations for students
Hey Michelle,
The easiest way to find out if it's the recycled regeants is to use fresh
reagents and see how the stain looks. Obvisously if it still shows sign of
a lack of color or detail than I would think it could be the stains
themselves.
What manufacture and type of Hematoxylin and Eosin ar
It is the most effective and economical way to run your IHC's.
We have been doing it that way for as long as I have been in histology.
Thanks,
Tim
- Original Message -
From:
To:
Sent: Tuesday, July 19, 2011 6:04 PM
Subject: Histonet Digest, Vol 92, Issue 25
Hi,
> I'm interested in kno
On all stomach biopsies.
Tim
- Original Message -
From:
To:
Sent: Wednesday, July 13, 2011 10:45 PM
Subject: Histonet Digest, Vol 92, Issue 18
Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://
My apologies, I wasn't ready for the number of responses I received over the
control tissue and might have missed some people. If I didn't contact you back
shoot me another email so I can start working on it.
Control Tissue still Available
Iron/FE
Tonsil
Hpylori
CD15/30
TTF1
p16
AFB
Fungus
> Last comment on the paraffin conversion for me.
>
> You can use bees wax if you want in your processor and embedder that
doesn't
> mean you going to get the optimal results for every situation. Paraffin
> types are a personal preference for most people or labs. It's a little
more
> hassle to k
I have an abundance of H-pylori controls that I would be willing to part with
and a number others as well.
Thanks,
Tim
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Hi Paula,
Obviously there are no "compression free" paraffin's on the market. Look
for a paraffin with a higher polymer content, this makes the paraffin firmer
and less likely to compress. Other factor for into section compression but
paraffin is a good place to start.
The higher polymer paraff
What is the purpose of batching control? Unless you are having a hard time
finding certain positive tissue types, it's good practice to run a positive
control with each case to ensure every slide shows the desired results.
Plus the patholigist have to call and verify that the control worked,
wasti
Hi Histonetters,
This maybe a stupid question but I'm going to ask anyways. Is it legal to
buy tissue blocks (with human tissue) from another lab to use as controls
for specials and Immunos? I know we purchase slides from vendors to use
with our specials and Immunos.
Thanks!
Tim
___
Glad I never worked at that lab, never would have made to a supervisory
role.
I have to agree with Sarah, two infractions in a 12 month period is
extremely severe. I don't care if you have Vantage or any one of the million
tracking software packages out there. Especially with the shortage of
qu
Hi Mala,
We've used the Fisher superfrost plus slides for years and have never had
that issue, it sounds more like you need to add some Tween to your buffer.
That will help the solutions spread along your slide.
Hope that helps.
Thanks,
Timothy Higgins, HT(ASCP) QIHC
-Original Message--
Agreed.
Thanks,
Timothy Higgins, HT(ASCP) QIHC
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tench, Bill
Sent: Friday, October 01, 2010 12:27 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet]
We use Statlab and Fisher marking inks. We dip our specimens in Bouin's or
Vinegar to set the ink. We don't have any problems with the ink coming off.
Hope that helps.
Thanks,
Timothy Higgins, HT(ASCP) QIHC
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:his
Hello Histonetters,
We are overstocked on Ultraview DAB detection kits and looking to sale some
for $2,300.00 per kit. We will pay for shipping within the continental US.
The kits are NOT registered with the button still attached.
We have 6 with the expiration Date: 4/2011
Lot #812955
We
Agreed!
Thanks,
Timothy Higgins, HT(ASCP) QIHC
Histology Manager
APA
Amarillo, TX
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A.
Powell
Sent: Monday, September 20, 2010 10:33 AM
To: Senn, Amy
Hello Histonetters,
We are in the process of selling some of our Ventana Ultraview Detection
kits. The kits still have a long shelf life and have not been registered.
If you are interested in purchasing one of more of these kits please email
me with questions and or contact info.
Thanks,
Tim
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