Subject: Re: [Histonet] IHC-start-up
Doing ihc on animal tissue is very tricky, if for no other reason than you are
trying to standardize an antibody across multiple species. You will need to use
every trick in the book and invent some of your own to get reproducible
results.
I would steer clear
9:45 AM
To: Erin Sarricks
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC-start-up
Doing ihc on animal tissue is very tricky, if for no other reason than you
are trying to standardize an antibody across multiple species. You will
need to use every trick in the book
1:10 PM
To: Colleen Forster
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC-start-up
Diagnostic Biosystems has the same platform with tech support that is
outstanding!
Michael O. Thompson
Director of Sales
Diagnostic BioSystems
Phone: 1-888-896-3350
Mobile: 412-860-1288
Office
Subject: Re: [Histonet] IHC-start-up
Diagnostic Biosystems has the same platform with tech support that is
outstanding!
Michael O. Thompson
Director of Sales
Diagnostic BioSystems
Phone: 1-888-896-3350
Mobile: 412-860-1288
Office Fax: 412-727-6080
IHC Made Affordable
www.dbiosys.com
Colleen
Hi,
there is disagreement at the our dept regarding the control slides for IHC, so
far on the control slides there is only writen control, marker and date of
staining (on automated stainer) but there is NOT indicated/writen the ID of
control, however I believe or it's obvious to me that is
Learn IHC staining, Hands - on IHC Lab courseCEU approved; February
27th 28th, 2014 San Francisco Bay area.
For more info contact Maria workshop...@gmail.com
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Hi All,
I'm currently recruiting for a new IHC tech with a facility located in San
Francisco, CA. Please find job description below:
IHC Histotechnologist/Histotechnician
Our Client is an exciting, high-growth molecular clinical laboratory located
in the Bay Area (Northern CA), utilizing
Good morning,
I know that this has been talked about on here before but I cannot find my
email regarding this issue. I need your help. When billing for IHC is the cpt
code 88343 pertaining to the dual or triple antibodies on one slide? 88342 for
the initial antibody? 88434 for each
Hello and happy new year! I was wondering what other labs (NOT CAP certified)
are doing for their daily QC sign off on each antibody run? I have about 5 docs
and each order anywhere from 2-50 IHC stains daily. Currently I use the Leica
Bond and I am pulling and printing case logs (brief slide
primarily during
validation. It is not too tedious if you have history.
Joelle Weaver MAOM, HTL (ASCP) QIHC
Date: Fri, 3 Jan 2014 11:22:38 -0500
From: ksime...@leavittmgt.com
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC QC
Hello and happy new year! I was wondering what other
Learn IHC staining, Hands - on IHC Lab courseCEU approved; February
27th 28th , 2014 San Francisco Bay area.
For more info contact Maria workshop...@gmail.com
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LEAD IMMUNOHISTOCHEMISTRY TECHNICIAN
ProPath, a progressive, CAP accredited, high-volume pathology practice in
Dallas, Texas is seeking an Lead Immunohistochemistry Technician for its'
Immunohistochemistry Lab. Responsibilities include slide preparation (paraffin
and frozen sections), IHC
Still looking for an technician to join a research team in Albany, NY in a
Neuropathology Lab devoted to hereditary ataxia research.
Duties include daily manual IHC/staining selected biomolecular techniques
(ELISA, PCR etc.). Looking to fill position immediately to replace associate
Hello everyone,
Got your Christmas shopping done? (Didn't think so...)
Anyway, I'm looking for an IHC PBS/Tween buffer wash concentrate that is
comparable to the very expensive Lab Vision PBS/Tween buffer wash. I see some
at VWR and FISHER (no shipping charges for our lab) that
You should try Idexx Laboratories!
I remain yours truely,
Candice Camille
From: Rob Day r...@foliobio.com
To: histonet@lists.utsouthwestern.edu
Sent: Friday, November 22, 2013 4:58 PM
Subject: [Histonet] IHC lab who can do Veterinary Markers?
You might
From: Rob Day r...@foliobio.com
To: histonet@lists.utsouthwestern.edu
Sent: Friday, November 22, 2013 4:58 PM
Subject: [Histonet] IHC lab who can do Veterinary Markers?
You might try www.phylogenyinc.com.
Looking for a lab that can do IHC on veterinary tissue
You might try www.phylogenyinc.com.
Looking for a lab that can do IHC on veterinary tissue of all types,
rodents(murine and rats), canine, feline, equine, avia as many as a
zoo would have.. Slides would be precut, usually with a control
already on the slide. Lookng
An academic research laboratory devoted to the study of hereditary ataxia and
located at the VA Medical Center in Albany, N.Y., is seeking a third research
associate.
Technologies include Routine and Advanced Immunohistochemistry of tissue
sections, Qualitative and Quantitative X-ray
IMMUNOHISTOCHEMISTRY TECHNICIAN
ProPath, a progressive, CAP accredited, high-volume pathology practice in
Dallas, Texas is seeking an Immunohistochemistry Technician for its'
Immunohistochemistry Lab. Responsibilities include slide preparation (paraffin
and frozen sections), IHC staining
In less than 8 hours I can get 96 Ihc stains done with our dako link and that
is two runs
Sent from my iPhone
On Oct 1, 2013, at 1:15 PM, McKenzie, Emily mckenzie.em...@mhsil.com wrote:
Hello all,
I am currently working on a Six Sigma Green Belt project and am looking for
some data. Can
Hello all,
I am wondering how many places offer same-day staining of IHC. We are a small
hospital based lab that averages about 70 IHC slides per week. We operate 5
days/week, 0600-1600. We give same day staining for IHC requests that are
received by 10am and are using the Ventana Benchmark
@lists.utsouthwestern.edu
Sent: Tuesday, October 1, 2013 11:00 AM
Subject: [Histonet] IHC trun-around-time
Hello all,
I am wondering how many places offer same-day staining of IHC. We are a small
hospital based lab that averages about 70 IHC slides per week. We operate 5
days/week, 0600-1600. We
Hello all,
I am currently working on a Six Sigma Green Belt project and am looking for
some data. Can any one give me their general turnaround times for IHC, from
time of order to time of delivery?
Thanks for all your help with this,
Emily K. McKenzie BS, HT(ASCP)
Memorial Medical Center│701
changes?
Regards
Gudrun Lang
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Tom
McNemar
Gesendet: Dienstag, 01. Oktober 2013 17:00
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] IHC trun
Dear Tom
Upgrade to the benchmark Ultra and your problem is solved as you can add and
remove slides continuously throughout the day. There is no run and no need
for cut offs. Our upgrade was cost neutral.
Regards
Gary Gracie
Senior Technical Officer
IHC Laboratory
Anatomical Pathology
St
I'm currently recruiting for an IHC Manager for a facility in southern San
Francisco. ASCP Certification is required. Feel free to contact me directly
with any questions.
Anna Nolan
Recruiter
Prometheus Healthcare
Direct Line 301-693-8908
Office 301-693-9057
Fax 301-368-2478
Good morning,
I would like to get some input on the type of tissue everyone is using for
their IHC MMR protocol. I would like to use just one block for all four
protocols if possible. Any suggestions.
: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Colleen
Forster
Sent: Thursday, August 15, 2013 5:59 PM
To: Histonet
Subject: [Histonet] IHC after EDTA decalcifying
Hello fellow histonetters,
Can someone who has worked with decalcifyied bone tell
Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
koelli...@comcast.net
Sent: Thursday, August 15, 2013 10:17 PM
To: Colleen Forster
Cc: Histonet
Subject: Re: [Histonet] IHC after EDTA decalcifying
Could I ask exactly
Hello fellow histonetters,
Can someone who has worked with decalcifyied bone tell me if EDTA
interferes with IHC staining? I was under the impression it did not but
cannot get staining in mouse femurs that I have decaled in EDTA. I have
used both the steamer and the decloaker for retrieval
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Colleen Forster
Sent: Thursday, August 15, 2013 4:59 PM
To: Histonet
Subject: [Histonet] IHC after EDTA decalcifying
Hello fellow histonetters,
Can someone who
but my control was also a
mouse.
Ray
Research Scientist
Comparative Medicine
University of Washington
- Original Message -
From: Colleen Forster cfors...@umn.edu
To: Histonet histonet@lists.utsouthwestern.edu
Sent: Thursday, August 15, 2013 4:58:35 PM
Subject: [Histonet] IHC
Dorothy:
I believe that the conference you're referring to is the Annual
International Retreat on Applied Immunohistochemistry and Molecular Pathology,
which
is sponsored by an organization called Pathology Learning Center.
Information relating to the meeting held this past February can
If there is anyone out there who is using Thermo's Autostainer and also using
their antibodies, would you mind contacting me offline? We are just starting
up IHC's, and I would appreciate some feedback and information on a few issues
I am having. Thanks.
Laurie Colbert, HT (ASCP)
Histology
Hello
I was just wondering if anyone had a link to any papers discussing the
loss of antigenicity with decomposition. I work with forensic tissue
which is often quite autolysed.
Thanks in advance
Finlay
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Hi CJ,
we had this problem the year before last, it turned out to be the slides.
Apparently, the manufactor had a couple of bad runs. We switched to Avantik
Biogroup Ultra Bond and haven't had a problem since. The bonus was they were
more reasonably priced compare to our contract vendor.
Hi All,
I'm currently recruiting for an IHC Manager in San Francisco, CA. Preferable
candidate will have:
-ASCP certification
-be local to the area/ willing to relocate without assistance
- 5 years minimum experience in histology/IHC
-6 months of management experience (preferred but not
So, I am working on some IHC stuff. I need control for mouse tissues. While I
have just about every normal tissue you could ask for, several of these
antibodies say the positive control is lung cancer, or breast cancer, or
prostate cancer. While I have all of these in human I don't have them
Do any of you do this? Do any referral labs offer this?
Thanks,
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
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Are there any reference labs offering this on FFPE specimens?
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
___
Histonet mailing list
Give me more specifics, platforms? Manual? Theory? Or training checklists?
Maybe can help, finishing mine now
Sent from my Verizon Wireless 4G LTE Smartphone
- Reply message -
From: jmasla...@stpetes.org
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Tech Competency
Is anyone out there performing IHC with Mouse primaries on gerbil tissue???
If so what are you using for secondaries?
Amy S. Porter, HT(ASCP) QIHC
Michigan State University
Investigative HistoPathology Laboratory
William S. Spielman, Ph.D. - Director
Department of Physiology / Human
Anyone willing to share IHC Technician Competency checklist?
Thanks in advance
Joe Maslanka BS, CT,HT (ASCP)
Anatomical Pathology Technical Supervisor
St Peter's Hospital,MT 59601
(P)(406) 447-2406
(F)(406)444-2126
Give thanks for ALL things.
Kindness is the language the blind can see
I'm hoping Histonet can come through for me! I have an unusual case that I
need to run IHC on. Antibody is Pan Keratin Cocktail from Cell Marque with
recommended protocol on a Benchmark Ultra with i-view detection. Only problem
is, I only have two slides and they are not charged. The
mjdess...@commonwealthhealth.net
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, May 16, 2013 4:49 PM
Subject: [Histonet] IHC on non-charged slides
I'm hoping Histonet can come through for me! I have an unusual case that I
need to run IHC on. Antibody is Pan Keratin Cocktail from Cell
Michael
There is a procedure where you can transfer the sections to a charges slide
with a product called mount quick. You can check with newcomer supply they sell
it. I know of several labs that have used it on these occasions and it works
well.
Sent from my iPhone
On May 16, 2013, at 4:50
Yes, if the stain can wait get some mount quick from Newcomer. There is a
good chance the tissue will fall off if you proceed with the tissue on
regular slides. I would wait until I get the mount quick.
Everyone else reading this should order a tube now for emergencies. You
will find yourself
Hi Histonetters,
We are looking for a part time evening Histologist
to do IHC and light grossing in the Fort Myers Florida area. Riverchase
Dermatology is the largest most comprehensive skin care center in the area with
over 6 offices and growing. Here is a link to
Hello fellow Histonetters:
Does anyone have an IHC technician / specialist job description that they would
wish to share with me? I am in the process of creating a new IHC tech
specific position, so any help is much appreciated.
Thank you,
Vincent Rivera, HT (ASCP) QIHC
Histopathology
I need to talk to any histotechs/researchers out there who are doing IHC on
shellfish or fish. Please contact me personally at
powell...@mercer.edumailto:powell...@mercer.edu. Also any vendors who sell
detection systems for them, please contact me.
Shirley A. Powell, HT(ASCP)HTL, QIHC
histonet-boun...@lists.utsouthwestern.edu;
histonet-requ...@lists.utsouthwestern.edu
histonet-requ...@lists.utsouthwestern.edu
Sent: Thursday, April 4, 2013 5:23 PM
Subject: [Histonet] IHC (DAB) after HE?
Dear Histonetters,
I would like to perform IHC (DAB) after HE. Do I need to remove HE
; histonet@lists.utsouthwestern.edu;
histonet-boun...@lists.utsouthwestern.edu;
histonet-requ...@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC (DAB) after HE?
Yes, you need to remove it. Hydrate your sections and place them in a 1% aq.
acetic acid solution (it will be a weak acid solution
Of MARY T HODGES
Sent: Friday, April 05, 2013 8:44 AM
To: Rene J Buesa ; nmargar...@luriechildrens.org ;
histonet@lists.utsouthwestern.edu ; histonet-boun...@lists.utsouthwestern.edu ;
histonet-requ...@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC (DAB) after HE?
I have just ihc over
Dear Histonetters,
I would like to perform IHC (DAB) after HE. Do I need to remove HE? If answer
is YES, my question HOW? Can I ask the protocol for that?
Thanks in advance,
Naira
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No. Re-hydrating the tissue will remove most (if not all) of the eosin and the
hematoxylin will serve as your counterstain (although you may need to
counterstain again at the end of you protocol). If you use antigen retrieval
for an unstained slide, you will have to use it on the HE-stained
What is the best pipette to buy for IHC? What ul range is best used?
Thank you-
Sent from my iPhone
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
, Unit E
Longmont, CO 80504
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb
Sent: Monday, April 01, 2013 4:36 PM
To: histonet
Subject: [Histonet] IHC pipettes:
What is the best pipette to buy for IHC? What
...@lists.utsouthwestern.edu] On Behalf Of Jb
Sent: Monday, April 01, 2013 3:36 PM
To: histonet
Subject: [Histonet] IHC pipettes:
What is the best pipette to buy for IHC? What ul range is best used?
Thank you-
Sent from my iPhone
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Eppendorf
René J.
From: Jb craiga...@gmail.com
To: histonet histonet@lists.utsouthwestern.edu
Sent: Monday, April 1, 2013 6:36 PM
Subject: [Histonet] IHC pipettes:
What is the best pipette to buy for IHC? What ul range is best used?
Thank you-
Sent from my iPhone
I currently use a Dako Autostainer and we are dispensing 300uL of all reagents
to the slide(100uL per drop zone). I am just curious what others are dispensing
and if you've noticed any difference by changing the dispense volume in regards
to stain quality. Does anyone adjust for very large
Hi Tiana,
We just got a Biocare Intellipath FLX and it came set at 300ul too. I've
switched it to 150ul, dropping in the middle zone only and had no problems, may
see if I can go 100ul for smaller tissues, will up it for larger tissues.
I pap pen around the samples though to make sure that
Medical Center Drive TVC 4531
Nashville, TN 37232
ashley.trout...@vanderbilt.edumailto:ashley.trout...@vanderbilt.edu
Message: 21
Date: Fri, 15 Mar 2013 16:51:27 +
From: Laurie Colbert lcolb...@pathmdlabs.commailto:lcolb...@pathmdlabs.com
Subject: [Histonet] IHC validation
To: Histonet Post
: Fri, 15 Mar 2013 16:51:27 +
Subject: [Histonet] IHC validation
If a lab is just starting up IHC for the first time and has to validate both
the IHC stainer and the AB's/detection kit, does the validation have to be
done using actual patient cases - or can you use strictly control tissue
IMMUNOHISTOCHEMISTRY TECHNICIAN
ProPath, a progressive, CAP accredited, high-volume pathology practice in
Dallas, Texas is seeking an Immunohistochemistry Technician
for its' Immunohistochemistry Lab. Responsibilities include slide
preparation (paraffin and frozen sections), IHC staining
IMMUNOHISTOCHEMISTRY TECHNICIAN
ProPath, a progressive, CAP accredited, high-volume pathology practice in
Dallas, Texas is seeking an Immunohistochemistry Technician for its'
Immunohistochemistry Lab. Responsibilities include slide preparation
(paraffin and frozen sections), IHC staining
Dawson; histonet
Subject: RE: [Histonet] IHC negative controls
Can anyone tell me if JHACO CLIA are deferring to CAP's judgment that a
negative control is not needed when utilizing a polymer detection?
I assume that this is the case, but I'd like to be sure.
Short answer:
Don't bet the farm
-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Monday, November 05, 2012 12:56 PM
To: Morken, Timothy; 'Glen Dawson'; 'histonet'
Subject: RE: [Histonet] IHC negative controls
The thing is in my opinion that having the backing of CAP on this issue is a
good argument to make to CLIA
Ruegg; 'Glen Dawson'; 'histonet'
Subject: RE: [Histonet] IHC negative controls
Could be, but my point, maybe not clear in my answer, is that you should
check with your accrediting agency before an inspection and not assume that
they will follow what CAP has said it will do.
Tim
-Original
-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Glen
Dawson
Sent: Friday, October 26, 2012 8:45 AM
To: histonet
Subject: [Histonet] IHC negative controls
All,
Can anyone tell me if JHACO CLIA are deferring to CAP's judgement
an extensive
validation is adequate.
Good luck with your decision,
Linda
-Original Message-
From: Kuhnla, Melissa [mailto:melissa.kuh...@chsli.org]
Sent: Monday, October 29, 2012 5:23 AM
To: Sebree Linda A; Glen Dawson; histonet
Subject: RE: [Histonet] IHC negative controls
Very good
All,
Can anyone tell me if JHACO CLIA are deferring to CAP's judgement that a
negative control is not needed when utilizing a polymer detection?
I assume that this is the case, but I'd like to be sure.
Thank-you in advance,
Glen Dawson BS, HT(ASCP), QIHC
Histology Technical Specialist
-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Glen Dawson
Sent: Friday, October 26, 2012 8:45 AM
To: histonet
Subject: [Histonet] IHC negative controls
All,
Can anyone tell me if JHACO CLIA are deferring to CAP's judgement that a
negative
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Glen Dawson
Sent: Friday, October 26, 2012 6:45 AM
To: histonet
Subject: [Histonet] IHC negative controls
All,
Can anyone tell me if JHACO CLIA are deferring to CAP's judgement that a
negative control is not needed when
I'm in a new position and have to get our IHC paperwork CAP ready. I'm not
sure about something. When you put a new antibody dispenser (with a
different lot # than previously used) on the IHC stainer, what QC needs to
be done? Can the control run with a patient test serve as your QC, or does
it
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deloris
Carter
Sent: Monday, October 01, 2012 3:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC new reagent lots
I'm in a new position and have to get our IHC paperwork CAP ready. I'm
not sure about
IMMUNOHISTOCHEMISTRY TECHNICIAN
ProPath, a progressive, CAP accredited, high-volume pathology practice in
Dallas, Texas is seeking an Immunohistochemistry Technician for its'
Immunohistochemistry Lab. Responsibilities include slide preparation
(paraffin and frozen sections), IHC staining
I completely agree with what Teri has mentioned regarding the problems with
making absolute requirements for
histology positions. I've been searching for a histology job in San Francisco
I know a number of professional websites
that list histology jobs for clinical, research, Biotech
What criteria does everyone use to hire for working in your IHC department? I
am looking for feedback as to what qualifications are expected for IHC techs as
well as qualifications and expectations of the lead in IHC. Also, what title
do you have for the lead in IHC, technical specialist,
:17 PM
Subject: [Histonet] IHC lead
What criteria does everyone use to hire for working in your IHC department? I
am looking for feedback as to what qualifications are expected for IHC techs as
well as qualifications and expectations of the lead in IHC. Also, what title
do you have
Hi All,
i need you guys help.
I am doing IHC on 4% formalin fixed mice liver paraffins section and want to
stain for Phospho-JNK. I am using primary Antibody from cell signaling (
Cat.no-9251). i followed the protocol exactly same as they suggested but i
did't see any staining. I did antigen
IMMUNOHISTOCHEMISTRY TECHNICIAN
ProPath, a progressive, CAP accredited, high-volume pathology practice in
Dallas, Texas is seeking an Immunohistochemistry Technician for its'
Immunohistochemistry Lab. Responsibilities include slide preparation
(paraffin and frozen sections), IHC staining
Good Morning Histonet,
We are currently searching for an experienced Histotech who has a
background in IHC that would be interested in working with a global leader
in a field support role. The IHC Field Specialist will be the expert on the
company’s IHC products and will interact with clients
Our IHC tech is having trouble validating NAPSIN A, we are running it on a
benchmark XT. She's been working with tech support at Ventana to no avail.
Any suggestions would be appreciated.
Thanks
Joe Maslanka BS, CT,HT (ASCP)
Anatomical Pathology Technical Supervisor
St Peter's Hospital,MT
Hi guys,
We are preparing for CAP Inspection and we were suppose to write
procedues/policieson stuffs like Buffer water, positive controls, negative
controls. ETC.
So please I will appreciate it if somebody can get these procedure out to
me.
I really appreciate you guys.
Hello everyone,
Is there any cost per test calculator that can be used to determine the cost
per test for IHC? Thanks
Tunde Ajibade
CONFIDENTIALITY NOTICE: The documents accompanying this email transmission
contain confidential information belonging to the sender that is legally
Dear All,
I want to stain for inos, Dectin-1 and Hem OX-1 on formalin fixed paraffins
sections. I was told by some one these antibodies only work on frozen sections
and not on paraffin sections, but I see all these antibodies datasheet say it
work on paraffin sections as well.
Any input will
What has been people's experience with immunohistochemical staining for p21,
p27, and Retinoblastoma (RB) gene protein on formalin-fixed, paraffin-embedded
human tissue? Thank you.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Friday, May 25, 2012 11:25 AM
To: Histonet
Subject: [Histonet] IHC for p21, p27, and RB
What has been people's experience with immunohistochemical staining for p21,
p27, and Retinoblastoma (RB) gene
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
[rcar...@harthosp.org]
Sent: Friday, May 25, 2012 1:24 PM
To: Histonet
Subject: [Histonet] IHC for p21, p27, and RB
What
Hello,
I have a full time permanent IHC Specialist Job opening in East Texas. If
you are in East Texas or would be interested in relocating to East Texas
please message me for more details.
To view a complete list of Allied Search Partners current openings go to:
Hello all,
A year or so ago, we upgraded from a Benchmark XT to Benchmark Ultra.
For validation, we selected a variety of cases as usual and ran them on
both instruments before we retired the XT. Now, when we add a brand new
antibody, we again select a variety of cases, and once we're happy
...@lists.utsouthwestern.edu] On Behalf Of Dessoye,
Michael J
Sent: Friday, May 04, 2012 8:51 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Validation
Hello all,
A year or so ago, we upgraded from a Benchmark XT to Benchmark Ultra.
For validation, we selected a variety of cases as usual and ran them
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Dessoye,
Michael J
Sent: Friday, May 04, 2012 7:51 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Validation
Hello all,
A year or so ago
Allied Search Partners has been retained for the following searches. We have
openings in Immunohistochemistry and Cytotechnology. Please forward this
along to anyone who you know that would be interested in any of the
following positions. We do offer generous referral bonuses.
1. Please email a
Hello Everyone,
If you send IHC to a ref lab to do the technical components only and you have
pathologists onsite to interpret the slides, do you need the QC log for that
stain from that ref lab? If you already have both positive and negative control
slides from that ref lab.
Thank you.
Can I get the synoptic version of the IHC charge, changes? IE; each antibody
or each block?
Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical
Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050
l FAX 804-765-8852
Has anyone succeeded in doing IHC for interferon gamma on formalin-fixed,
paraffin-embedded tissue? Thank you.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour
1. Any feedback on the process for validating a new tissue processor? Finding
80 to 100 breast specimens that are large enough to divide up and retest
Estrogen and Progesterone would take a long time to complete. Also, how many
antibodies and specimens should be tested? As we all know, some
I am having to validate antibodies and was wondering if you have to do a
parallel study or just use known positive and negative tissue?
~Sabrina~
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...@marshfieldclinic.org wrote:
From: Bliven, Laura bliven.la...@marshfieldclinic.org
Subject: [Histonet] IHC Processor Validation MUM1
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Tuesday, March 13, 2012, 9:29 AM
1. Any feedback on the process for validating
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