Re: [Histonet] tissue highlighting for visibility
Drop of hematoxylin on the tissue, when put on the paper in the grossing area. Use a syringe. Only a SMALL drop. Too much means there's extra blue all over the paper, making it hard to see the blue tissue. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect those of Beaumont Hospital. -Original Message- From: cont...@histocare.com Sent: Tuesday, August 07, 2012 6:10 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue highlighting for visibility Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. What do some of you guys do? www.HistoCare.com Histology Staffing ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Teabags
Hint when using these - do NOT try to fold them up into a nice looking square. Once processed and in paraffin, it is very difficult to find the edge, to try to open back up. Fold into a not nice to look at, off-set square that is slightly crumpled. Much easier to find the edge. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect on Beaumont Hospital. -Original Message- From: Clouse, Rosanna Sent: Tuesday, August 07, 2012 3:31 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Teabags For those of you who like lens paper and/or the Obex Histo Wrap, a very inexpensive alternative is to visit any beauty store or visit sallybeauty.com and get a box of Jumbo End Wraps for $1.99 for a thousand 2.5 x 4 sheets. We have used them for years and they work really well for cell blocks. Rosanna S. Clouse, SCT(ASCP) Division Manager - Cytology Gettysburg Hospital - Wellspan Gettysburg, PA 17325 email-rclo...@wellspan.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, August 07, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Teabags Susan Walzer notes I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue. I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. __ CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . __ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] tissue highlighting for visibility
ditto on this. I also have very tiny specimens and this works wonderful, but use the smallest of drop Kate Mendell Histopathology/Lab Manager HOWARD S. GOLDBERG, M.D., INC 990 Paradise Road Swampscott, MA 01907 TEL: 781.595.0151 FAX: 781.592.6780 kmend...@goldbergmd.net www.cosmesticdermcenter.com PRIVACY NOTICE: This e-mail message may contain confidential patient or other information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or authorized entity named above. The authorized recipient of this patient or other confidential information is prohibited from disclosing the information to any other party. If you have received this message in error, please notify the sender immediately and delete. Please keep any information you may have viewed confidential. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk [lpw...@sbcglobal.net] Sent: Wednesday, August 08, 2012 5:33 AM To: cont...@histocare.com; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] tissue highlighting for visibility Drop of hematoxylin on the tissue, when put on the paper in the grossing area. Use a syringe. Only a SMALL drop. Too much means there's extra blue all over the paper, making it hard to see the blue tissue. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect those of Beaumont Hospital. -Original Message- From: cont...@histocare.com Sent: Tuesday, August 07, 2012 6:10 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue highlighting for visibility Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. What do some of you guys do? www.HistoCare.com Histology Staffing ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Processor
I am going to need to purchase a new tissue processor mine keeps breaking down. What tissue processor would you buy and why? I would greatly appreciate the help. Cheers, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] tissue highlighting for visibility
We use microwave processing and we add hematoxylin to the absolute and eosin to the isopropylthis also helps in keeping techs from accidently using isopropyl as absolute or vice-versa... when grosser cant find tissue in the container we put a drop of eosin and swirl and filter to try and find any material in the formalin container. Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vpe...@pathreflab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of MaryK Mendell Sent: Wednesday, August 08, 2012 5:48 AM To: Lee Peggy Wenk; cont...@histocare.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] tissue highlighting for visibility ditto on this. I also have very tiny specimens and this works wonderful, but use the smallest of drop Kate Mendell Histopathology/Lab Manager HOWARD S. GOLDBERG, M.D., INC 990 Paradise Road Swampscott, MA 01907 TEL: 781.595.0151 FAX: 781.592.6780 kmend...@goldbergmd.net www.cosmesticdermcenter.com PRIVACY NOTICE: This e-mail message may contain confidential patient or other information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or authorized entity named above. The authorized recipient of this patient or other confidential information is prohibited from disclosing the information to any other party. If you have received this message in error, please notify the sender immediately and delete. Please keep any information you may have viewed confidential. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk [lpw...@sbcglobal.net] Sent: Wednesday, August 08, 2012 5:33 AM To: cont...@histocare.com; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] tissue highlighting for visibility Drop of hematoxylin on the tissue, when put on the paper in the grossing area. Use a syringe. Only a SMALL drop. Too much means there's extra blue all over the paper, making it hard to see the blue tissue. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect those of Beaumont Hospital. -Original Message- From: cont...@histocare.com Sent: Tuesday, August 07, 2012 6:10 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue highlighting for visibility Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. What do some of you guys do? www.HistoCare.com Histology Staffing ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tissue Processor
Sakura René J. From: Heckford, Karen - SMMC-SF karen.heckf...@dignityhealth.org To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, August 8, 2012 8:11 AM Subject: [Histonet] Tissue Processor I am going to need to purchase a new tissue processor mine keeps breaking down. What tissue processor would you buy and why? I would greatly appreciate the help. Cheers, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] decalcification of premolar teeth (dog)
Alice I would recommend using sodium formate/formic acid mixture for demineralization as this is more gentle than most agents. I would not use hydrochloric acid unless you are shipwrecked on a desert island and that is the only chemical available to you. I am assuming that EDTA demineralization is not an option for you? Barry From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Alice Fraser [toxpat...@gmail.com] Sent: Tuesday, August 07, 2012 8:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decalcification of premolar teeth (dog) Dear Members I would be really interested to hear your advice on the currently preferred procedure for decalcification of premolar teeth from dogs. Do laboratories find the HCl/water solution or the formic acid solution or another solution to be optimal for decal without obliterating the tissues to be evaluated? A procedure/method would be hugely appreciated if poss. Many thanks. Alice ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] tissue highlighting for visibility
I always used few drops of alcoholic eosin in the 70%EthOL, just enough to make the solution a pale pink. That amount is enough to give a faint pink hue to the tissue to ease its localization. This does not interfere with any stain done after wards. René J. From: cont...@histocare.com cont...@histocare.com To: Histonet@lists.utsouthwestern.edu Sent: Tuesday, August 7, 2012 6:10 PM Subject: [Histonet] tissue highlighting for visibility Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. What do some of you guys do? http://www.histocare.com/ Histology Staffing ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] help ! paraffin section
What you describe is a typical example of poor paraffin infiltration = the paraffin has not infiltrated the tissue and when you prepare the final block it will consist of 2 different components; the tissue and the paraffin. That is why you end with a good paraffin section without the tissue. Poor paraffin infiltration is always caused by an improper sequence while tissue processing. Either the fixation is incomplete OR the dehydration is incomplete and there is water in the tissue when you go to the clearing stage OR the clearing stage is incomplete and the tissue still has alcohol (immiscible with paraffin) when the tissue goes to the paraffin OR the paraffin infiltration is too short. The problem resides in your processing protocol and there is nothing you can do about that at the end. Try to check your processing protocol to eliminate the problem. If this is happening all of the sudden while you used to have good results previously, then you either have changed reagents or the reagents are not in a good condition. René J. From: Megha Kumar meg...@g.clemson.edu To: histonet@lists.utsouthwestern.edu Sent: Tuesday, August 7, 2012 11:45 PM Subject: [Histonet] help ! paraffin section Hi All I am trying to section adult mouse intestine and skin using paraffin embedding. However, when i section, the tissue is torn although the rest of the paraffin looks perfect. Please suggest why this is happening. Also, sometimes the skin sections fall off the slides when I perform in situ hybridization. Any ideas how to prevent this? Please help! i am a beginner in histology and dont' know what to do! regards Megha * * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] help ! paraffin section
In addition to Rene's comment,to cut coagulated tissue (skin that have new wound crust) and calcified tissue is difficult. On Wed, Aug 8, 2012 at 6:45 AM, Megha Kumar meg...@g.clemson.edu wrote: Hi All I am trying to section adult mouse intestine and skin using paraffin embedding. However, when i section, the tissue is torn although the rest of the paraffin looks perfect. Please suggest why this is happening. Also, sometimes the skin sections fall off the slides when I perform in situ hybridization. Any ideas how to prevent this? Please help! i am a beginner in histology and dont' know what to do! regards Megha * * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-173/237 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ki67 antibody to stain Mouse tissue
Just to clarify. Are you using it on mouse tissues? I am only asking because the Thermo Scientific/Labvision/Neomarkers data sheet says it has only been verified on Human tissues. Secondly had anyone used it on mouse gut. Does it stain in the bottom of the crypts or at the top? I am asking because I have also tried a different antibody from Leica but got staining at the top of the crypts not at the bottom. Thank you to everyone for all of your valuable suggestions and advice, Eva Permaul Georgetown University On Tue, Aug 7, 2012 at 2:21 AM, Bruijntjes, J.P. (Joost) joost.bruijnt...@tno.triskelion.nl wrote: Hi Eva I've used an antibody from Labvision /Neomarkers. It is a rabbit monoclonal; catalog number: RM-9106. Joost -Oorspronkelijk bericht- Van: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] Namens Eva Permaul Verzonden: maandag 6 augustus 2012 20:44 Aan: histonet Onderwerp: [Histonet] Ki67 antibody to stain Mouse tissue Hello, I am looking for a Ki67 to stain mouse tissues. We have been using Dako M2749 but just found out it has been discontinued. Could anyone suggest a good alternative? Thank you all for any help, Eva Permaul Georgetown University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 105, Issue 9
We make up a marking Eosin that we use on small bx before we place them in cassettes. We do not use actual teabags, but the mesh bags from Thermo Fisher that are not hard to pull apart and we never have anything sticking I the corners. I do know what you are referring to as some of the mesh bags ot teabags on the market are not mad of as fine material and are hard to pull apart and allow for specimens to gather in the corners. Our mesh bags are used for all specimens the PA's can pour into the bags. We also use the Obex papers for needle bx type specimens that can be laid out nicely on the paper and folded over the tissue once for optimal processing. On tissue processors, we are new this year to the Peloris processor from Leica and love the versatility of two retorts and the great reagent management which allows for less waste and less maintenance tech time. I was always a Sakura VIP fan previous, still am, but cannot compare the two retort option and how LEAN it has mad our lab! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, August 08, 2012 9:49 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 105, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Re: opinion on heating slides prior to IHC (Teri Johnson) 2. Manual for Reichert-Jung/Leica Cryocut 1800 Cryostat with 2020 microtome? (Jennifer Johnson) 3. Re: Teabags (Bob Richmond) 4. RE: Re: Teabags (Sarah Dysart) 5. RE: Re: Teabags (Clouse, Rosanna) 6. Re: Re: Teabags (Paula Pierce) 7. RE: Re: Teabags (Shirley A. Powell) 8. tissue highlighting for visibility (cont...@histocare.com) 9. decalcification of premolar teeth (dog) (Alice Fraser) 10. National Society for Histotechnology Hard Tissue Forum Event - Bethesda, MD on August 18, 2012!!! (Jack Ratliff) 11. help ! paraffin section (Megha Kumar) 12. Re: tissue highlighting for visibility (Lee Peggy Wenk) 13. Re: Re: Teabags (Lee Peggy Wenk) 14. RE: tissue highlighting for visibility (MaryK Mendell) 15. Tissue Processor (Heckford, Karen - SMMC-SF) 16. RE: tissue highlighting for visibility (Vanessa Perez) 17. Re: Tissue Processor (Rene J Buesa) 18. Re: tissue highlighting for visibility (Rene J Buesa) 19. RE: decalcification of premolar teeth (dog) (Rittman, Barry R) 20. Re: help ! paraffin section (Rene J Buesa) -- Message: 1 Date: Tue, 7 Aug 2012 17:30:04 + From: Teri Johnson tjohn...@gnf.org Subject: [Histonet] Re: opinion on heating slides prior to IHC To: jefthomp...@salud.unm.edu jefthomp...@salud.unm.edu Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 9f3cfee76e51b64991c7485270890b400cdcb...@ex4.lj.gnf.org Content-Type: text/plain; charset=us-ascii Dear J, I agree with Rene Busa on his assessment that thawing and refreezing is very very bad for your epitopes. You don't do it with antibodies, you don't want to do it with your antigens either. Keep the slides you will not need frozen, taking out only what will be needed. Work quickly as exposure to the air will start the condensation process. I also agree that there does not need to be an ethanol rehydration step. That might be useful as a permeabilization step, but you only need to hydrate your tissue with buffer. You might not have realized that alcohol can permeabilize cells (even though they are already exposed through sectioning) and also affect the protein folding, so if your antibodies are already working using this scheme you might see a difference if you quit doing it. You also might not, so it could be useful to test it. As to whether to use heat to thaw, you can try putting the slides in front of fans at room temperature, or you can try fan forced ovens set at 25 or even 37 degrees if you are worried about heat. I have worked with a researcher who used unfixed cryosections of brain and put them in a 95 degree oven for 2 minutes prior to freezer storage. They were still able to get antibody and mRNA staining from their sample. I have also read accounts of people using a hair dryer (blow dryer) on the heat setting on them as well with no ill effect on their published studies. Who is to
Re: [Histonet] Ki67 antibody to stain Mouse tissue
I just found a nice publication with the Rm-9106 on mouse intestine. Have provided it to my manager. Thank you everyone. Eva On Wed, Aug 8, 2012 at 11:38 AM, Eva Permaul e...@georgetown.edu wrote: Just to clarify. Are you using it on mouse tissues? I am only asking because the Thermo Scientific/Labvision/Neomarkers data sheet says it has only been verified on Human tissues. Secondly had anyone used it on mouse gut. Does it stain in the bottom of the crypts or at the top? I am asking because I have also tried a different antibody from Leica but got staining at the top of the crypts not at the bottom. Thank you to everyone for all of your valuable suggestions and advice, Eva Permaul Georgetown University On Tue, Aug 7, 2012 at 2:21 AM, Bruijntjes, J.P. (Joost) joost.bruijnt...@tno.triskelion.nl wrote: Hi Eva I've used an antibody from Labvision /Neomarkers. It is a rabbit monoclonal; catalog number: RM-9106. Joost -Oorspronkelijk bericht- Van: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] Namens Eva Permaul Verzonden: maandag 6 augustus 2012 20:44 Aan: histonet Onderwerp: [Histonet] Ki67 antibody to stain Mouse tissue Hello, I am looking for a Ki67 to stain mouse tissues. We have been using Dako M2749 but just found out it has been discontinued. Could anyone suggest a good alternative? Thank you all for any help, Eva Permaul Georgetown University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ki67 antibody to stain Mouse tissue
FYI: That is what the data sheet says because they didn't test it but many of us have and it works beautifully! It should stain mostly in the bottom of the crypts where the new cells emerge from but you will have occasional cells towards the top of the crypt. It should look much like it does in human gut and yes, I have done it on most major organs of the mouse. Colleen Forster U of MN On 8/8/2012 10:38 AM, Eva Permaul wrote: Just to clarify. Are you using it on mouse tissues? I am only asking because the Thermo Scientific/Labvision/Neomarkers data sheet says it has only been verified on Human tissues. Secondly had anyone used it on mouse gut. Does it stain in the bottom of the crypts or at the top? I am asking because I have also tried a different antibody from Leica but got staining at the top of the crypts not at the bottom. Thank you to everyone for all of your valuable suggestions and advice, Eva Permaul Georgetown University On Tue, Aug 7, 2012 at 2:21 AM, Bruijntjes, J.P. (Joost) joost.bruijnt...@tno.triskelion.nl wrote: Hi Eva I've used an antibody from Labvision /Neomarkers. It is a rabbit monoclonal; catalog number: RM-9106. Joost -Oorspronkelijk bericht- Van: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] Namens Eva Permaul Verzonden: maandag 6 augustus 2012 20:44 Aan: histonet Onderwerp: [Histonet] Ki67 antibody to stain Mouse tissue Hello, I am looking for a Ki67 to stain mouse tissues. We have been using Dako M2749 but just found out it has been discontinued. Could anyone suggest a good alternative? Thank you all for any help, Eva Permaul Georgetown University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tissue Processor
We have swithched from the VIP Sakura, older models, to the Thermo Excelsior. We are currently buying our 3rd and 4th units for the Histology Lab. They have features we really liked and cut my our exposure to formalin and xylene by almost 90% due to the way the system changes solutions. Due to the very gently agitation used during processing we were also able to cut some time off our programs for overnight and short cycle biopsy runs. We stil use a Leica ASP 300 for bone marrows only as it is what need due to reagent requirements for xylene sub on the last two stations. If you have any questions let me know. Pam Marcum UAMS - Original Message - From: Karen - SMMC-SF Heckford karen.heckf...@dignityhealth.org To: histonet@lists.utsouthwestern.edu Sent: Wednesday, August 8, 2012 7:11:13 AM Subject: [Histonet] Tissue Processor I am going to need to purchase a new tissue processor mine keeps breaking down. What tissue processor would you buy and why? I would greatly appreciate the help. Cheers, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] tissue highlighting for visibility
We just add about 10-15 ml of eosin in the last alcohol on the processor Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of cont...@histocare.com Sent: Tuesday, August 07, 2012 6:10 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue highlighting for visibility Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. What do some of you guys do? www.HistoCare.com Histology Staffing ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Ki67 to stain mouse tissue
We've had good success with clone SP6, a rabbit monoclonal from Vector. This antibody works in many species and has been very reliable. Tim Coskran Pfizer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Free-standing Dictation System for AP
Dear Histonet members, Good afternoon! I was wondering if anyone can kindly recommend a free-standing electronic dictation system to be used with an Anatomic Pathology information systems? Any suggestions or advice would be greatly appreciated. Thank you, Mari Mari Yang, MHA, CT(ASCP)CMHTLCM Cytology Supervisor Tel: 760.773.2009 P Save a tree, please don't print this e-mail unless you really need to. Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
