Re: [Histonet] Microtomes

[Cooper, Brian via Histonet]

This has been a Histonet post in the past; techs are fiercely loyal to their 
favorite brand and I'm sure you'll see people disagree with me as soon as I hit 
send!

I prefer Microm.  In our experience, block alignment is easier with the 
Microm's because the X and Y axis orientation knobs are positioned in such a 
manner that you can adjust block orientation with your left hand while your 
right hand is on the flywheel.  This allows you to keep a constant sight line 
of the entire depth of the block face in relation to the knife.  With the Leica 
microtomes, the positions of these screws are moved to the right, and the 
orientation of the Y axis requires you to either use two hands at once, or for 
you to move your left arm across your sight line to orient the Y axis.  While 
certainly not insurmountable; this just makes block orientation take a little 
longer.  Since we frequently have to recut blocks from different institutions, 
this is a big deal here.  I'm sure the peeps from the other camp are going to 
say that it's all in the technique.  To that I say, YEAH, YEAH, YEAH  :)

Both of these machines are well designed and will provide years and years of 
excellent service as long as they are well maintained.  The best thing you can 
do is ask to demo both machines and see what you like best. 

Good luck!

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu 


-Original Message-
From: HERRINGTON, SHEILA via Histonet 
[mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, January 27, 2016 11:52 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtomes

We are looking to purchase two new microtomes and were wondering on thoughts 
and recommendations from the experience of the people that actually use them.  
Pros and cons would be extremely valuable on ergonomics, reliability and 
overall performance.

Sheila Herrington, RT
Technical Lead, Immunohistochemistry and Histopathology Kelowna General Hospital
2268 Pandosy Street,
Kelowna, B.C. V1Y 1T2

250-862-4300 x 7510

sheila.herring...@interiorhealth.ca



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[Histonet] ORO staining in pre- vs. post-fixed tissue

[Michele Wich via Histonet]

Does anyone know whether there is a difference, quantitatively, in Oil red O 
fat staining of tissue fixed in NBF prior to embedding in OCT vs. fresh frozen 
tissue embedded in OCT? I know that sections from fresh frozen tissues must be 
post-fixed in formalin before staining with Oil Red O. But is there any benefit 
to using this preparation over tissues that are fixed in formalin prior to OCT 
embedding (aside from sectioning/slide adhesion issues previously described)? 
Is the staining similar, or does one preparation give better staining results?
Much gratitude for any advice you can give,
Michele

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[Histonet] Tissue Arrays

[Amos Brooks via Histonet]

Hi,
 My experience with microarrays is that they are sometimes a bit
complicated to work with depending on the way they are constructed and the
platform they are used on. Often these slides are dipped in paraffin to
help preserve them. When this happens they need considerably longer
deparaffinization. They also sometimes are not floated on a waterbath to
transfer them to the slide but by a tape transfer process. The tape often
interferes in IHC staining and especially on Ventana platforms that make
use of the liquid coverslips. If you can try to get microarrays that are
floated like regular sections, make sure you give them a good long soak in
xylene, and perhaps increase the volume per slide of the antibodies.

Best of luck,
Amos


On Wed, Jan 27, 2016 at 1:00 PM, 
wrote:

> Message: 5
> Date: Wed, 27 Jan 2016 14:15:05 +
> From: John Shelley 
> To: "histonet@lists.utsouthwestern.edu"
> 
> Subject: [Histonet] Tissue Arrays
> Message-ID:
> 
> Content-Type: text/plain; charset="us-ascii"
>
> Good Morning,
>
> I have purchased some arrays from a company in the past and had some very
> varying results. I prefer not to mention the company and so my request is
> if those of you who do not have the luxury of having tissue at your
> disposal to make your own tissue arrays from whom are you buying them from.
> Once purchased have you used them on IHC platforms like Ventana
> Benchmark/Ultra or Leica Bond3? Were your results consistent across the
> slide?
>
> I would like to add that I am looking for neuroblastoma tumor arrays. Any
> help will be greatly appreciated. Thanks in advance!
>
> Kind Regards!
>
> John J Shelley
>
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[Histonet] Laboratory clerical job position *Delray Beach, FL*

[Delray Beach Pathology Kari Simeone via Histonet]

Hi Histonetters. I thought maybe I'd share a position we have available in our 
dermatology laboratory that is NOT TECHNICAL. Maybe you can share with someone 
who might be looking for a job in our area of Delray Beach, Florida. It is M-F 
9-5p, FULL TIME clerical work. 401k, benefits and vacation. 25k annually. 
Filing, phones, doctor interaction/assisting and data entry. Must take drug and 
personality tests along with a background check.



No experience necessary, WILL TRAIN. Please click on link to apply.



https://advancedderm.applicantpro.com/jobs/242606.html



Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor

Alternate Laboratory Supervisor

Delray Beach Technical Laboratory

ADCS Clinics, LLC



www.advancedderm.com





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[Histonet] EWSR1 FISH positive tissue request

[Jennifer Valentine-Williams via Histonet]

Hello Histoland,

I'm reaching out in hopes that someone may have some unstained slides of Ewing 
Sarcoma FFPE they can share with me.  I'm working on a validation and it had 
proved to be a difficult task in finding a positive case for FISH.

If you have anything that you could share, I can provide you with our FedEx 
account for shipping.

Thanks in advance!


Jennifer Valentine-Williams, HT(ASCP)


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[Histonet] Alliance Rubber Bands

[Brent Adams via Histonet]

Hey Histonetters,



I don't endorse products but this one is really good.



I don't get a kick back and no relatives work for the company, I just wanted

to give you all a good product to use if you are looking. I looked up the 
company

and found they are the last U.S. Made Rubber Band company and based out of

Arkansas.



I think I have had 2 eye injuries and multiple painful mishaps from

rubber bands that break far to easily so from that perspective I feel

I can give this product a thumbs up.



For the last 4 years I have been using  Alliance Rubber Company

non- latex rubber bands. The size 64 (3.5 x 1/4) work best on Slide folders

I have found. The Orange color rubber bands are easy to see and

hold the file folders together great and they rarely break.



I know it sound silly to talk about rubber bands this way but I was

really getting tired of being snapped by faulty rubber bands when trying to

wrap slide folders or paperwork.



I just had my first rubber band by Alliance snap a couple months ago which 
caught me

off guard cause it had been so long since that happened. It was then I look to

see what brand we were using and it turned out that we had been using the same

NON-latex rubber bands from the Alliance Rubber Company since I opened the 
Laboratory in 2012.

Before that at other labs breaking rubber bands were just part of the daily Job.



I think purchasing has gotten their best price on Amazon but they also use 
staples and

Office depot for supplies. I informed them that I only want these rubber bands 
and they

can get their best pricing where ever.



My fingers hurt a lot less after distributing slide folders and I don't have to 
swear as much. Ha Ha.

Funny how little things can really bother us.



FYI



Brent Adams - BS, LPN, HT


www.acadianagastro.com

Acadiana Gastroenterology Associates, LLC
439 Heymann Blvd
Lafayette, LA  70503

tel:  (337) 269-1126
fax:  (337) 269-1476
PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein 
are confidential and protected from disclosure pursuant to Federal Law. This 
message is for the designated recipient only and may contain confidential, 
privileged, proprietary, or otherwise private information. If you have received 
this email in error, please notify the sender immediately and delete the 
original with any attachments. Any other use of the email is strictly 
prohibited.
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[Histonet] Part Time Histotech Job in West Chicago-Permanent Placement

[Melissa Owens via Histonet]

Hello Histoland,

Just an quick note to say that I am currently looking for a qualified Histotech 
to work in a part time position for the evening shift on a permanent basis. 
This position is in West Chicago, IL. Please contact me for more details. Have 
a great day!

Melissa Owens
President, Laboratory Staffing
Allied Search Partners

T: 888.388.7571 ext. 102

F: 888.388.7572

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[Histonet] Microtomes

[HERRINGTON, SHEILA via Histonet]

We are looking to purchase two new microtomes and were wondering on thoughts 
and recommendations from the experience of the people that actually use them.  
Pros and cons would be extremely valuable on ergonomics, reliability and 
overall performance.

Sheila Herrington, RT
Technical Lead, Immunohistochemistry and Histopathology
Kelowna General Hospital
2268 Pandosy Street,
Kelowna, B.C. V1Y 1T2

250-862-4300 x 7510

sheila.herring...@interiorhealth.ca



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Re: [Histonet] Alliance Rubber Bands

[Cooper, Brian via Histonet]

Awesome post Brent!   I thought I was the only one who had a strong opinion 
about rubber bands! I never used the orange color bands you mentioned, but the 
Alliance Pale Crepe Gold #64 were pretty awesome too.  Agree totally--cheap 
rubber bands HURT

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Brent Adams via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, January 27, 2016 11:29 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Alliance Rubber Bands

Hey Histonetters,



I don't endorse products but this one is really good.



I don't get a kick back and no relatives work for the company, I just wanted

to give you all a good product to use if you are looking. I looked up the 
company

and found they are the last U.S. Made Rubber Band company and based out of

Arkansas.



I think I have had 2 eye injuries and multiple painful mishaps from

rubber bands that break far to easily so from that perspective I feel

I can give this product a thumbs up.



For the last 4 years I have been using  Alliance Rubber Company

non- latex rubber bands. The size 64 (3.5 x 1/4) work best on Slide folders

I have found. The Orange color rubber bands are easy to see and

hold the file folders together great and they rarely break.



I know it sound silly to talk about rubber bands this way but I was

really getting tired of being snapped by faulty rubber bands when trying to

wrap slide folders or paperwork.



I just had my first rubber band by Alliance snap a couple months ago which 
caught me

off guard cause it had been so long since that happened. It was then I look to

see what brand we were using and it turned out that we had been using the same

NON-latex rubber bands from the Alliance Rubber Company since I opened the 
Laboratory in 2012.

Before that at other labs breaking rubber bands were just part of the daily Job.



I think purchasing has gotten their best price on Amazon but they also use 
staples and

Office depot for supplies. I informed them that I only want these rubber bands 
and they

can get their best pricing where ever.



My fingers hurt a lot less after distributing slide folders and I don't have to 
swear as much. Ha Ha.

Funny how little things can really bother us.



FYI



Brent Adams - BS, LPN, HT


www.acadianagastro.com

Acadiana Gastroenterology Associates, LLC
439 Heymann Blvd
Lafayette, LA  70503

tel:  (337) 269-1126
fax:  (337) 269-1476
PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein 
are confidential and protected from disclosure pursuant to Federal Law. This 
message is for the designated recipient only and may contain confidential, 
privileged, proprietary, or otherwise private information. If you have received 
this email in error, please notify the sender immediately and delete the 
original with any attachments. Any other use of the email is strictly 
prohibited.
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is for the sole use of the intended recipient(s) and may contain confidential
or legally privileged information. Any unauthorized review, use, disclosure
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message.  

-


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[Histonet] Tissue Arrays

[John Shelley via Histonet]

Good Morning,

I have purchased some arrays from a company in the past and had some very 
varying results. I prefer not to mention the company and so my request is if 
those of you who do not have the luxury of having tissue at your disposal to 
make your own tissue arrays from whom are you buying them from. Once purchased 
have you used them on IHC platforms like Ventana Benchmark/Ultra or Leica 
Bond3? Were your results consistent across the slide?

I would like to add that I am looking for neuroblastoma tumor arrays. Any help 
will be greatly appreciated. Thanks in advance!

Kind Regards!

John J Shelley

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