Re: [Histonet] Iron Stain
Try your eosin stain used in your H or 1% Neutral red in 2% acetic acid Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: Campbell, Tasha M. via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, 8 August 2018 9:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Iron Stain Is nuclear fast red the only counter stain for Prussian blue stain? I have a Masson's trichrome kit and was wondering if the Bierbech Scarlet could be a counterstain. I won't be doing the iron stain very often at all so I am trying to keep dry reagents on hand to make up as needed so they do not expire so quickly. Thanks! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Buffy Coat Subspecialty
Good afternoon, Does anyone know the correct subspecialty for Buffy Coats on the CLIA certificate? Any feedback would be appreciated! Caroline M. Pratt, MBA Practice Administrator Dermpath 3020 Market Street, Ste 201 Philadelphia, PA 19104 Phone 215-349-8178 Cell 610-800-1381 Fax 215-662-6150 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Iron Stain
HI Tasha, The nuclear fast red will stain the nuclei red, the biebrich scarlet is a connective tissue stain and may cover up the (sometimes sparse) prussian blue localization of iron in the tissues. I, personally, would not consider these reagents swappable. In my experience nuclear fast red does last a long time, but I am also not in a lab where expiration dates matter. mills ᐧ On Wed, Aug 8, 2018 at 4:52 AM Campbell, Tasha M. via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Is nuclear fast red the only counter stain for Prussian blue stain? I > have a Masson's trichrome kit and was wondering if the Bierbech Scarlet > could be a counterstain. I won't be doing the iron stain very often at all > so I am trying to keep dry reagents on hand to make up as needed so they do > not expire so quickly. Thanks! > > > > > Tasha Campbell, B.S.,HTL(ASCP) > Frederick Gastroenterology Associates > 310 W. 9th St. > Frederick, MD 21701 > 301-695-6800 ext. 144 > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Biology www.3Scan.com 415 2187297 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Iron Stain
Oops, yes, I am mixing up neutral red - and the recipe from Bryan is what I use - it last forever. I have had luck with nuclear fast red from vector - comes made up. That lasts a little longer than lab made. mills ᐧ On Wed, Aug 8, 2018 at 12:52 PM Caroline Miller wrote: > HI Tasha, > > The nuclear fast red will stain the nuclei red, the biebrich scarlet is a > connective tissue stain and may cover up the (sometimes sparse) prussian > blue localization of iron in the tissues. I, personally, would not consider > these reagents swappable. > > In my experience nuclear fast red does last a long time, but I am also not > in a lab where expiration dates matter. > > mills > ᐧ > > On Wed, Aug 8, 2018 at 4:52 AM Campbell, Tasha M. via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > >> Is nuclear fast red the only counter stain for Prussian blue stain? I >> have a Masson's trichrome kit and was wondering if the Bierbech Scarlet >> could be a counterstain. I won't be doing the iron stain very often at all >> so I am trying to keep dry reagents on hand to make up as needed so they do >> not expire so quickly. Thanks! >> >> >> >> >> Tasha Campbell, B.S.,HTL(ASCP) >> Frederick Gastroenterology Associates >> 310 W. 9th St. >> Frederick, MD 21701 >> 301-695-6800 ext. 144 >> >> ___ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > -- > Caroline Miller (mills) > Director of Biology > > www.3Scan.com > 415 2187297 > > > -- Caroline Miller (mills) Director of Biology www.3Scan.com 415 2187297 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Iron Stain
1% neutral red in 0.01% acetic acid (1 mL of 1% per 100 mL stain) works, as does 1% safranin. Both are stable, although should be filtered from time to time. Stain 1 minute, water rinse, ethanol, xylene and mount should work. I had one pathologist who preferred a very light progressive H She said she was interested in increased amounts of iron and normal amounts not being glaringly obvious didn't bother her. Bryan Llewellyn Bob Richmond via Histonet wrote: Tasha Campbell, B.S.,HTL(ASCP), at Frederick Gastroenterology Associates in Frederick, Maryland asks: Is nuclear fast red the only counter stain for the Prussian blue stain? I have a Masson's trichrome kit and was wondering if [Biebrich] scarlet could be [used as] a counterstain. I won't be doing the iron stain very often at all so I am trying to keep dry reagents on hand to make up as needed so they do not expire so quickly.<< This old pathologist never found Biebrich scarlet or the various fuchsins to be satisfactory substitutes for nuclear fast red - they're too dense, and they don't stain nuclei specifically. Nuclear fast red (Kernechtrot, C.I. 60760) is a dye with problems - poor shelf life both dry and in solution, and it will probably go out of manufacture eventually. It's used as an aluminum lake for staining nuclei. I'm not sure what would serve as an alternative. I'd like to know more about brazilin (particularly about Anatech's Brazilliant), a catechol dye similar to hematoxylin, with good specificity for nuclei. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Iron Stain
Tasha Campbell, B.S.,HTL(ASCP), at Frederick Gastroenterology Associates in Frederick, Maryland asks: >>Is nuclear fast red the only counter stain for the Prussian blue stain? I have a Masson's trichrome kit and was wondering if [Biebrich] scarlet could be [used as] a counterstain. I won't be doing the iron stain very often at all so I am trying to keep dry reagents on hand to make up as needed so they do not expire so quickly.<< This old pathologist never found Biebrich scarlet or the various fuchsins to be satisfactory substitutes for nuclear fast red - they're too dense, and they don't stain nuclei specifically. Nuclear fast red (Kernechtrot, C.I. 60760) is a dye with problems - poor shelf life both dry and in solution, and it will probably go out of manufacture eventually. It's used as an aluminum lake for staining nuclei. I'm not sure what would serve as an alternative. I'd like to know more about brazilin (particularly about Anatech's Brazilliant), a catechol dye similar to hematoxylin, with good specificity for nuclei. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Iron Stain
Is nuclear fast red the only counter stain for Prussian blue stain? I have a Masson's trichrome kit and was wondering if the Bierbech Scarlet could be a counterstain. I won't be doing the iron stain very often at all so I am trying to keep dry reagents on hand to make up as needed so they do not expire so quickly. Thanks! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
