Charles,
I am in a research only lab as well. We create TMA's as control blocks
using the same idea that Val uses. Multiple tissues per block for all IHC
and also good for special stains. You can make nicely organized TMA's
manually very cost effectively as well. Feel free to reach out to me if
Paula,
I start my processor at 80%. I am in research and often the samples have
been fixed and are put into 70-80% ethanol for holding or transport.
Starting with 80% steps right in line with the samples. I rarely see a
schedule that starts at 50%. However, I do have a couple labs that ask me
for
I agree. Making some of these simple solutions that need to be FRESH is the
better way to go. As John said, there are ma ny books with these formulas
in them.
I have used Davidson's for different projects and I make mine in the lab
fresh each time tissue is harvested.
Colleen Forster
You are very welcome!
Colleen
On Tue, Nov 7, 2023 at 4:02 PM Naira Margaryan
wrote:
> Awesome, thank you so much Colleen, for such quick response,
> Naira
>
> On Tue, Nov 7, 2023 at 4:01 PM Colleen Forster wrote:
>
>> Naira,
>>
>> Contact Lee Dickey with Ted Pella. They carry the whole line
Naira,
Contact Lee Dickey with Ted Pella. They carry the whole line of large block
adapters for the Leica microtomes.
lee_dic...@tedpella.com
I have this set up and it works great.
Colleen Forster HT(ASCP)QIHC
On Tue, Nov 7, 2023 at 3:58 PM Naira Margaryan via Histonet <
I would love too but unable to move the whole family!
Colleen Forster
On Fri, Oct 27, 2023 at 11:20 AM Stephanie L. Thompson via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Come to beautiful New Hampshire, the best beaches, Great White Mountains,
> no income or state taxes, quality
Even 30um is too thick for the routine IHC on glass. You will never
penetrate the whole thickness for staining.
Colleen Forster HT(ASCP)QIHC
On Fri, Aug 4, 2023 at 2:05 PM Alonso Martínez Canabal <
acana...@ciencias.unam.mx> wrote:
> Hi, thank you for your reply.
> I cannot do my usual free
Alonso,
For sections that thick you would collect them as you always have, stain
them as floating sections and then mount onto a glass slide. The routine
standard methods for IHC will never penetrate a 50um thick section.
Colleen Forster HT(ASCP)QIHC
On Fri, Aug 4, 2023 at 1:17 PM Alonso
I run a research lab with automated staining and truthfully,I make my
dilutions fresh for each run because proteins are so different in their
stability.
Colleen Forster HT(ASCP)QIHC
On Sun, Jul 2, 2023 at 7:08 PM Tony Henwood via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> In
Hello Histologists,
I am wondering if any of you will share your SOP for changing solurions on
processors and stainers.
How many slides or samples do you put through before you rotate or totally
change out solurions. I want to get as many answers as possible so I can
put together a table for
In my experience if the antibody are designed for flow they rarely work on
tissue samples.
Colleen Forster HT(ASCP)QIHC
University of MN
On Wed, Aug 24, 2022 at 1:56 PM Charles Riley via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> I have been given an antibody that is used for flow
Donna,
I know first hand, if you have any computer issues that take out your
software, ( power surge for example) Leica does NOT reinstall the program.
You are left to find a different program. It took us a long time to get a
program so we could use both the IPC/IPS again. The new software isn't
HEllo Histonetters,
We are a research lab that has been using the Leica IPC/IPS printed for
many years. Today the flash motor went out in the IPS. We are now looking
into updating the slide printer.
We are research only and NOT hooked into any LIS system. It would be a
stand alone process. Can
ith specific
> cryo attachments is used.
>
> Sincerely,
>
> Paula Sicurello
>
> Sent from my iPhone
>
> > On May 18, 2022, at 12:55 PM, Colleen Forster via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> >
> > FollowingI am in r
FollowingI am in research and I have never cut this cold.
Colleen Forster
On Wed, May 18, 2022 at 2:04 PM Ken Marzinsky via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Can anyone tell me why research labs often require cryostat cutting head
> temperatures down to -60C?
>
> Ken
>
Great advice Tim. I still do this 30 years later. It definitely expands
your scope of work, keeps the job interesting and builds your resource
network
Colleen Forster HT(ASCP)QIHC
On Mon, May 9, 2022, 5:38 PM Tim Morken via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Pam, I tell
Make sure the periodic acid is made fresh EACH time you run the stain.
That can also make a big difference in the stain quality.
Colleen Forster HT(ASCP)QIHC
On Thu, Sep 23, 2021 at 6:14 PM Tony Henwood (SCHN) via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> I agree with Bryan,
>
>
I agree with Terri. I also have an 1850 and will use it until parts are no
longer available which could be,. like she said, years. We also have a
local company do the PM and repairs and will use it until
it absolutely cannot be repaired.
Colleen Forster HT(ASCP)QIHC
University of Minnesota
BLS
I'd love to join you but I will be working that entire day
Colleen Forster
On Wed, May 19, 2021 at 3:24 PM Lauren Sweeney via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Hi Everyone,
>
> It is not too late to join us for the GSH Virtual Summit on May 22, 2021
> beginning at 8:00
Wow, what an excellent list John. I didn't ask the question but will surely
use the response. Thank you for sharing.
Respectfully,
Colleen Forster HT(ASCP)QIHC
University of Minnesota
On Mon, May 3, 2021 at 12:32 PM John Kiernan via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
>
Cheryl,
When I have this I use a small cube of tissue that is completely unrelated
to the project as my marker. You can even ink all surfaces on this small
piece of tissue. I process something but don't block it and keep that piece
to cut the smaller cubes from.
I keep track on a "map" and then
Hello Histonet,
Can you give me feedback on trying to do IHC on samples fixed in Zamboni's
fixative? I have an idea but need confirmation .
Thank you in advance
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
I use charged slides all the time. The best way to clear the water is to
cut the slides and air dry them overnight. The next day I follow the normal
protocol, heat in 60 degree over and then on to the rest of the protocol.
They don't really need the 56 degree oven all night long.
Respectfully,
Rhinda,
Which Alkaline Phos detection? How old is the kit?
I have found that the Alk Phos detection can die before the stated out
date. I use a Biocare platform as well. I have learned to leave the
chromagen 4 degrees until I am ready to stain. I make what I need and
immediately [lace the whole
HEllo Histoneters,
I am looking at replacing my VIP2000, porr girl finally quit on me.
The two processors I am looking at:
Leica ASP300S
Sakura VIP5
Any of you out there who have used either of these or both, can you give me
pros , cons, yes, noI am just looking for experiences those who
Totally following this one...
And what do you use to record humidity and what would be the cut off for
good staining , especially for IHC stains.
Colleen Forster
On Wed, Aug 19, 2020 at 6:30 PM Patti Nelson via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Hello Histo peeps
>
> I
Terri,
I agree with John on the use of Histogel. I use it routinely for all the
cell blocks and tiny matrix samples that come down and I get excellent
results.
I do a lot of IHC and the results are good.
Respectfully,
Colleen
On Thu, Jan 23, 2020 at 11:51 AM John Garratt via Histonet <
The Biocare certain was called the Nemesis. They are phasibg them out but
might be willing to come assist you.
Colleen Forster
On Thu, Oct 24, 2019, 2:43 PM Eileen Akemi Allison via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Ask Biocare or Dako. Unbeknownst to most of the public,
I'd love to know who actually told Michael this? I've had a company try
this on me as wellinterested to know if it is the same company.
C. Forster
On Thu, Sep 12, 2019 at 12:27 PM Maria Cruz via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Mr. Bradley:
> This is how awful rumors
Wow John,
Great information.,always so much to learn!~
Thank you,
Colleen Forster
U of MN
On Tue, Sep 10, 2019 at 10:55 AM Morken, Timothy via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> John, we love it when you "ramble!" It gives us an appreciation for the
> history and
We cut alot if these. This is the protocol:
1. The microtome, blade and water bath are cleaned well. We follow
withRNAse Away wipe down as an added precaution.
2. We use a coplin jar of 100% ethanol for forceps, pick or whatever other
tool you might use that comes into contact with and dip and
I agree with Brett.
I believe the histological aspect will be horrible and probably not worth
the time and money spent. I feel you would be terribly disappointed with
the lack of usable results.
Colleen Forster HT(ASCP)QIHC
On Thu, Aug 22, 2019 at 11:14 AM Brett Connolly via Histonet <
Biocare Medical Promark series are also great animal polymer based
detection that work very well.
https://biocare.net/products/detection/
Colleen Forster HT(ASCP)WQIUC
U of MN
On Wed, Jun 12, 2019 at 10:42 AM Paula Keene Pierce via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Vector
Following...
On Friday, April 19, 2019, Wellen, Terry :LLS Lab via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Would anybody share which antibodies they use for their CK Pools?
> Some questions have been raised by some of our newer Pathologists.
>
>
> Thanks,
>
> Terrence D. Wellen HT
Barbi,
When we designed ours we had multiple samples that included: neg, 1+,2+,
3+, 4+ both ER and PR and a second one had those plus the Her2 the same...
C
On Wed, Mar 13, 2019 at 12:44 PM Moe, Barbi A via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> For anyone making in-house ER/PR
YEs, it isI have done it and it is a heavy load...and in this case
completely unnecessaryshe is qualified without having to be a Med
Tech...goodness
C
On Thu, Feb 21, 2019 at 11:07 AM Perl , Alison wrote:
> Fortunately our company has tuition reimbursement, so one of my techs is
>
Wow...this is just crazy~ The regulations leave histology in a real tough
spot...
Feeling for all of you and yes, for the future histology techs coming up~
Colleen Forster
U of MN
On Thu, Feb 21, 2019 at 10:52 AM Perl , Alison via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Hi
Yes! Your expertise is such a valuable resource..
C
On Wednesday, February 13, 2019, Bob Richmond wrote:
> You're welcome!
>
> I sat down and read through most of it as soon as I saw it. So glad John
> Kiernan's back!
>
>
> On Wed, Feb 13, 2019 at 8:48 PM Colleen Forster wrote:
>
>> THis
THis looks likje a great resource...I will take time to look it over.
Thank you for all your valuable mentorship over the years
Respectfully,
Colleen Forster
U of MN
On Wed, Feb 13, 2019 at 4:35 PM Bob Richmond via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> John Kiernan,
following.
I haven't done frozen stains on GP only FFPE...interested in what others
have to say.
Colleen
On Tue, Jan 29, 2019 at 1:58 PM Jan Shivers via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Has anyone ever performed IHC on frozen sections of guinea pig tissue? I
> am
Hello Dave,
I still do a lot of manual work and I also use Biocare reagents. They work
well and their customer service is very good.
Colleen Forster
On Fri, Jan 18, 2019 at 12:58 PM Jeanine Ronkowski via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Hi Dave:Our lab has used reagents
I am with Jennifer on the IntelliPath.I have just gotten 2 of them in
my lab.
The new instrument was demoed at NSH last year. It is an amazing
machine,I wish I could have had that one VEry limited numbers will be
avaiable in November with the main stream sales beginning in January.
This sounds wonderful..I wish I were in a position to swoop it up!
Colleen Forster HT(ASCP)QIHC
On Wed, Jun 6, 2018 at 9:24 AM, Arrington, Karla A via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Hello All,
>
> I hope everyone is enjoying the early part of summer.
>
> In beautiful
On Tue, May 1, 2018 at 12:07 PM, shultz11 via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
>
> Leica equipment is now being made in China not Germany. I wish I would
> have known this before purchasing equipment.
> Kendra ShultzSent from my Verizon, Samsung Galaxy smartphone
>
To do samples that you suspect prion disease also needs to be done in a
lab specifically designed for that.
I agree, formalin is sufficient.,
Colleen
On Mon, Apr 23, 2018 at 11:14 AM, Rene J Buesa via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> I believe that is an extreme and
Following...would like to see the answer.
Colleen Forster
On Tue, Mar 27, 2018 at 3:25 PM, Karl Koessler via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Does anyone know of a stain or marker I can use to differentiate
> sympathetic from parasympathetic in sacral spinal cord sections?
I agree Terry,
The TMA slide is a very economical and powerful way to validate with
minimal slides needed.
Colleen Forster
On Tue, Mar 20, 2018 at 12:54 PM, Terri Braud via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Just another note: You can order unstained tissue microarrays
Greetings Histonet family,
I am looking for a good reference for the hsitology of the Drosphilia fly.
I am cutting then and need a reference to correlate my stains to. It
wouild be really nice if they showed color p[lates done with H/E stain.
Does anyone have a good reference they can
Greetings Histonetters,
Our facility is looking into setting up a hard tissue service. I am in need
of a resource that I can talk to for advice on the equipment and supplies
we would need to have in a preliminary budget proposal.
Any hard tissue experts out there willing to assist me?
Thank
Can anyone give me a processing schedule for Drosophila flies...very tiny.
Thank you in advance!
Colleen Forster HT(ASCP)QIHC
U of MN
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Hello Jennifer,
I am located in Minneapolis, MN so they would have to ship them to me. The
turn around time is very doable.
Let me know if they would even consider my lab before I go through the work
of a cost estimate.
We have done projects like this before, I would love the opportunity to
You are welcomeit should be the original one by SIgnet...then
COvancenow BioLEgends
C
On Wed, Dec 27, 2017 at 3:34 PM, Cartun, Richard <
richard.car...@hhchealth.org> wrote:
> No, I would like to use “6E10” if it’s still available. Thank you!
>
>
>
> *Richard*
>
>
>
> *From:*
Do you knot want to use 6E10 anymore? I believe you can still get it from
Biolegends?
https://www.biolegend.com/en-us/products/purified-anti-beta-amyloid--1-16-antibody-11228
Respectfully,
Colleen Forster HT(ASCP)QIHC
*University of Minnesota*
On Wed, Dec 27, 2017 at 3:20 PM, Cartun, Richard
gt;
>
> On Tuesday, December 19, 2017 9:45 AM, Colleen Forster via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
>
>
> Be sure you are processing them on an overnight process. Even if the
> samples are small it takes that time to process the agar properly.
>
>
Be sure you are processing them on an overnight process. Even if the
samples are small it takes that time to process the agar properly.
I use Histogel and learned this lesson the hard way. My samples always
"shriveled" up too until someone enlightened me about the processing time.
I would think
I am following, would love to see the responses.
Colleen Forster
On Thu, Sep 28, 2017 at 5:14 PM, Judi Ford via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Hi Everyone,
> I am interested in connecting with techs/managers who are working in or
> have developed a translation
Cristi,
I follow the general guidelines of good fixation for the msajority. IF I am
testing something that has specific in the clinicla lab, I try it the same
on animal tissues for the same thing.
Colleen Forster HT(ASCP)QIHC
On Thu, Jun 15, 2017 at 4:05 PM, Eddie Martin via Histonet <
Following.
Colleen Forster
On Mon, May 29, 2017 at 10:04 AM, Gregoire, Rhonda (AGR) via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Does anyone have any experience processing and paraffin embedding
> butterflies?
>
> Rhonda Gregoire, MLT
> Supervisor, Clinical Pathology
>
Thanks Gaylethis is the system the pathologist wanted to
but$3000.00 and he had no money
I want to try and work with more reasonably priced ideas first.not sure
I will ever get this one downI think Ill have better luck with bugs!
I will watch this video and read the notes
This sounds like a wonderful opportunity.I wish I were in a position to
go for it!
C
On Mon, Aug 15, 2016 at 12:43 PM, Lila Adams via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Good afternoon everyone,
> I thought I would post this job opportunity.
> CV Path Institute Job
Brett,
Are you interested in sending all mof them as a complete (-7 issues) set?
Colleen Forster
U of MN
On Tue, Aug 9, 2016 at 8:57 AM, Connolly, Brett M via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> HI all,
>
> Is anyone interested in old issues of JOH? My collection spans
Brett,
I use the rabbit monoclonal CD3 from Thermo (it was a Lab Vision antibody)
with great and consistent results!
The hardest part, actually getting the antibody to me\jeez they have
problems with orders sometimes...
Here is the information for mine:
CD3 (SP7) - cat #RM-9107-S1
I use
I find the same as Caroline Miller, about 20-25% with formalin fixation and
routine processing.
C
On Mon, Feb 22, 2016 at 3:41 PM, Caroline Miller via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> hi All, I have done some experiments in this area for mouse brains, and I
> find that
This looks wonderful!
Be sure to keep me on your list Pam so I can consider this meeting!
C
On Thu, Jan 28, 2016 at 2:50 PM, Pamela Marcum via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
>
>
> Good Afternoon,
>
>
>
>
> We would like to invite all of you to Branson Missouri for the
I think that is about average for good Tshirts these days.
C
On Tue, Jan 26, 2016 at 2:43 PM, Bitting, Angela K. via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Is it me or does anyone else think $20 is a lot of money for a T-shirt?
> The Histotechnology Professionals Day T-shirts on
I am interested to see what you hear BrettI need to do these same
markers and am not having good luck in FFPEfrozens , great...FFPE NOT!
C
On Tue, Nov 3, 2015 at 7:32 AM, Connolly, Brett M via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Hi all,
> I have a project that will be
I'd be very interested in this as well...could you shaere Karen.
Thanks.
C
On Mon, Nov 2, 2015 at 11:43 AM, Karen Cai via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> HELP:
>
>
>
> Hi,
>
> Is there anybody can provide me the price list/structure of the custom IHC
> services?
>
>
>
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