[Histonet] Qiagen Qproteome FFPE Tissue Kit

Hello, Has anyone tried the Qproteome FFPE Tissue Kit kit from Qiagen: Qproteome FFPE Tissue Kit For isolation of full-length proteins from formalin-fixed tissues * Standardized, quantitative extraction from FFPE tissue * Full-length proteins * Low sample requirements The

[Histonet] COL3A1

Hello, All, Curious if anyone knows of a great COL3A1 (Collagen III) antibody that works well in IHC for paraffin sections for human skin. I have tested a few and the ones that work/stain seem to stain most of the dermis of the human skin and I am concerned that they are staining for other

[Histonet] keratohyalin granules

Hello All, Does anyone have experience staining the keratohyalin granules in skin with different types of hematoxylins? I am currently looking at keratohyalin granules and staining with Harris hematoxylin and there seems to be little staining and not as prominent as I would have expected.

[Histonet] X-gal staining

Hello All, Does anyone know of a commercially available X-gal stain that would work on formalin fixed paraffin embedded skin samples in order to detect beta galactosidase. Thank you in advance. Kris ___ Histonet mailing list

[Histonet] papillary dermis

Hello All, Does anyone know of a technique, special stain, biomarker, etc. that will somehow demonstrate the difference between papillary dermis and reticular dermis in skin. Estimating where the reticular dermis ends and the reticular dermis ends is very subjective. Any help would be

[Histonet] whole slide scanners

Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] procollagen 1

Hello All, I have been using a procollagen antibody from Millipore and the latest lots now do not work. I am in the middle of running a clinical study which has a deadline that is closing in and would greatly appreciate if anyone could recommend a good procollagen antibody for skin. This

[Histonet] Biocare Medical Mach 4

Hello All, Does anyone have any knowledge of the Biocare Medical Mach 4 AP detection system. How does this system exactly work. Does it use a dextran backbone with 2ndary antibodies attached like other systems and then the polymer binds to that? Their datasheets simply state that it uses a

[Histonet] Herovici stain

All, Does anyone know what is the chemistry/biology that lets the stain selectively bind mature/premature collagen? I have tried looking for any literature dealing with this topic but have come up empty. If anyone can give me any information or point me in the right direction of where to

[Histonet] elastic stain

All, I have used a new Verhoeff-Van Gieson stain kit (from BBC) for elastic fibers and have run a large number of slides. I am noticing differences in the staining intensities throughout the samples. I see that there are darker sites and lighter sites within the same panelists. This is not

[Histonet] Herovici stain

Is anyone familiar with Herovici stain? If so, does anyone know of a commercial vendor for this stain or does anyone have a protocol that they would be willing to share? I would like to stain some skin samples and compare collagen 1 and collagen 3 within the dermis. Thank you in advance.

[Histonet] elastic fiber stain in dermis of skin

I am currently running a study to determine the increase in elastin in the dermis of skin due to certain treatments applied to skin. These samples will be FFPE after the study concludes. Does anyone have suggestions on which type of elastin stain works best for FFPE skin samples? Are there any

[Histonet] sectioning frozen skin

Can anyone suggest what the optimal thickness of flash frozen skin sections should be cut at? I typically cut FFPE sections at 5 um, should frozens be cut thinner or thicker? These sections will be used for HE and routine IHC. Thank you in advance. Kris

[Histonet] flash freezing tissue

Can someone recommend the best protocol for flash freezing tissue (skin). I am unsure if fixing tissue before freezing or fixing tissue post freezing is best. Also, these samples cannot be fixed with formalin. Thanks in advance. kris ___ Histonet

[Histonet] hematoxylin-eosin saffron

Can someone please explain to me the purpose of a hematoxylin-eosin saffron stain. Why would this be used over the normal hematoxylin and eaosin stain. Thank you in advance. Kris ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] fontana

Does anyone know of anything other than melanin that Fontana Masson would stain? Also, does anyone know of a stain that would label un/saturated fatty acids? Not sure if this is even possible. Thanks in advance. Kris Kalleberg ___ Histonet mailing

[Histonet] Biopsy fixation

Hello All, I have a simple fixation question. Typically when we process 3 mm skin biopsies we will fix in 10% NBF for 4 hours and then wash in PBS and then process over night into paraffin and then embed. If we are unable to immediately process the 3mm skin biopsies after 4 hr fixation what is

[Histonet] automated immunostainer

All, I am looking for an automated immunostainer for research purposes. I would like something reliable, easy to use, and compatible with a wide variety of reagents and protocols. Does anyone have any recommendations? We are considering upgrading our lab from a manual lab to fully automated.

[Histonet] flash frozen tissue

Hello All, I will be running a study upcoming and we have decided not to run FFPE as we normally do and have decided to use flash frozen. My big concern is what is the proper way to fix all of these SKIN samples to use for IHC. Any recommendations as to if prefix the samples in sucrose and

[Histonet] incubator oven

Hi All, I desperately need to get a new incubator oven for my histology lab. It seems as if most ovens are now convection ovens. Since my old oven is not convection I am just concerned that the constant air movement will some how affect the tissue slides in the way the paraffin in melted