Re: [Histonet] vasculature staining in 3D

[Paula Keene Pierce via Histonet]

Hi,
I have done retinal flat mounts to visualize vasculature with lectins and used 
chromagenic instead of fluoro.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Wednesday, March 13, 2024 at 12:44:36 PM CDT, M.O. via Histonet 
 wrote:  
 
 Happy Wednesday!

I have an image in my head and I cannot find any image that matches what I
saw - cleared 3D tissue, maybe it was lungs or a heart with blue
vessels/veins stained.

I've done skeletal staining on mouse embryos with alcian blue and alizarin
red and was wondering if there is something similar for soft tissue and
vessels (not fluorescent). Is this something that exists?

I've seen vascular casting, but couldn't find images of cleared tissue with
stained vessels.

Thank you for any hints to my mystery!
-Merissa
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Re: [Histonet] Histonet Digest, Vol 243, Issue 6

[Paula Keene Pierce via Histonet]

When I need to handwrite on slides or cassettes, I use Sakura Pigma Micron 
archival ink pens.
They come in different tip sizes and colors and are found at any office supply 
store.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Thursday, February 8, 2024 at 01:43:43 PM CST, KATHLEEN FERNANDEZ via 
Histonet  wrote:  
 
 Paula,
We are using the KP’s at our lab too and have been noticing that they are 
smearing! We are having issues on our slides, since we hand write still. This 
just recently started happening. Maybe they changed their formula?

Kathy
Sent from my iPhone

> On Feb 8, 2024, at 12:01 PM, histonet-requ...@lists.utsouthwestern.edu wrote:
> 
> Send Histonet mailing list submissions to
>    histonet@lists.utsouthwestern.edu
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> To subscribe or unsubscribe via the World Wide Web, visit
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> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>  1. Marking Pen (Paula)
>  2. Re: Marking Pen (Piche, Jessica)
>  3. Lead Histotechnologist (Stephanie L. Thompson)
> 
> 
> --
> 
> Message: 1
> Date: Wed, 7 Feb 2024 11:35:26 -0800
> From: "Paula" 
> To: 
> Subject: [Histonet] Marking Pen
> Message-ID: <009e01da59fc$ce13b680$6a3b2380$@biopath.org>
> Content-Type: text/plain;    charset="us-ascii"
> 
> Hello,
> 
> 
> 
> We have been using KP Marker Plus pens for cassettes.  We have 2 Leica
> Processors and 1 VIP6 processor.  The cassettes that go into the VIP6 are
> smeared and some are almost smeared off completely.  The processors have the
> same solutions in them.
> 
> 
> 
> If anyone can shed some light as to why there is a difference, and if anyone
> can recommend a better marking pen for us to try, I would appreciate the
> feedback.
> 
> 
> 
> Thank you,
> 
> Paula
> 
> 
> 
> --
> 
> Message: 2
> Date: Thu, 8 Feb 2024 11:30:03 +
> From: "Piche, Jessica" 
> To: "histonet@lists.utsouthwestern.edu"
>    ,    Paula 
> Subject: Re: [Histonet] Marking Pen
> Message-ID:
>    
>
>    
> Content-Type: text/plain; charset="us-ascii"
> 
> Hi Paula,
> 
> What is the first solution the cassettes go in when the processor starts? We 
> use Statmark pens when our cassette printer isn't working, and they work 
> well. The only time we have had issues was when we would hand write the 
> cassettes and run them on our small biopsy run which skips the formalin. It 
> seems like the marker ink needs to "fix" with the formalin. Sometimes they 
> smear if they aren't dry enough before they go into formalin too. I hope you 
> figure it out. Maybe see if you can get some samples of different pens and 
> then run some empty cassettes and see what works best for you.
> 
> Have a good day.
> 
> Jessica
> 
> Jessica Piche, HT(ASCP)
> Waterbury Hospital Histology Laboratory
> Histology Team Leader
> 203-573-7167
> 
> From: Paula via Histonet 
> Sent: Wednesday, February 7, 2024 2:35 PM
> To: histonet@lists.utsouthwestern.edu 
> Subject: [Histonet] Marking Pen
> 
> [EXTERNAL MSG]
> 
> Hello,
> 
> 
> 
> We have been using KP Marker Plus pens for cassettes.  We have 2 Leica
> Processors and 1 VIP6 processor.  The cassettes that go into the VIP6 are
> smeared and some are almost smeared off completely.  The processors have the
> same solutions in them.
> 
> 
> 
> If anyone can shed some light as to why there is a difference, and if anyone
> can recommend a better marking pen for us to try, I would appreciate the
> feedback.
> 
> 
> 
> Thank you,
> 
> Paula
> 
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> 
> 
> --
> 
> Message: 3
> Date: Thu, 8 Feb 2024 17:19:28 +
> From: "Stephanie L. Thompson" 
> To: "Histonet@lists.utsouthwestern.edu"
>    
> Subject: [Histonet] Lead Histotechnologist
> Message-ID: 
> Content-Type: text/plain; charset="us-ascii"
> 
> Looking for the next step in your career?
> 
> Sonic 

Re: [Histonet] Microcredentials for Histology

[Paula Keene Pierce via Histonet]

Here! Here!
I sure would not want the pilot flying a plane I was on to have micro 
credentials.
Like, hey I learned how to raise the landing gear this week. 
Standards should be raised, not doing just enough to get by.
In histology, all steps in totality create the final product.
I still think blocks and slides should be sent in as part of the registry, as 
we did in the past.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Thursday, November 2, 2023 at 10:14:07 AM CDT, Jay Lundgren via Histonet 
 wrote:  
 
 What Jennifer said.

Just what kind of jumped-up osteopath college are you people running up
there?  Shocking.

You think they're looking for "microcredentials" at M.D. Anderson or
Brigham and Women's?

You're not going to help the shortage or the field by lowering standards.
Just kill a bunch of patients.

You want to attract registered/licensed HT/Ls?  PAY MORE MONEY!

You call yourself a Medical School (since 2018), act like one.

Please show this email to your boss.

Sincerely,

Jay A. Lundgren, M.S., HTL (ASCP)




Virus-free.www.avast.com

<#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2>

On Fri, Oct 27, 2023 at 3:39 PM Mac Donald, Jennifer via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> With route 2 the applicants must have at least one year of full-time
> acceptable experience working histology with experience in microtomy,
> embedding, fixation, staining, and lab operations.  They will be required
> to provide proof of the employment/experience to the ASCP with their
> transcripts.
>
> -Original Message-
> From: Kara, Phillip via Histonet 
> Sent: Friday, October 27, 2023 9:34 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Microcredentials for Histology
>
>  EXTERNAL SENDER - Exercise caution with requests, links, and attachments.
>
> Good morning everyone.
>
> I recently started a position with the University of North Texas Health
> Science Center as their Histology Research Associate. During a quick
> meeting with my director he mentioned that he was interested in possibly
> setting up a 'microcredentialing' program for Histology since our lab is
> now up and running.
> Pretty much from what he was telling me it would be similar to when we
> were in high school taking those computer classes where we would get the
> Microsoft certifications showing we knew how to use Word, Excel,
> Powerpoint, ect.
> My hope would be to try setting it up in such a way that students when
> finished would have a better understanding of what we do in Histology but
> also have the basics to possibly sit (depending on how long out program
> would last) for the HT exam using the route 2 option ["Both require prior
> education in the histology field, either through attending a histology
> program, or acquiring
> laboratory experience in the field."] My question for the field is 2 fold.
>
>  1.  Is this something that you think could be a second path for people
> to get into the field of Histology due to our nation wide shortage? We deal
> with med students, pre med, medical research students, and starting a
> nursing program here next year.
>  2.  If this could work what do you think I should focus on? My initial
> thoughts were of course sectioning, processing, maybe even some staining.
> But what else?
>
> Interested to hear everyone's thoughts on the topic.
>
> [
> https://mail.google.com/mail/u/0?ui=2=8e07797374=0.1=msg-f:1778656250049141922=18af0e6f89b51ca2=fimg=ip=s0-l75-ft=ANGjdJ8LWmuH5_BzU-y7vwwBOWcW_Ue6DO0UFBd0pTuQUq3tVH-HDPbJqzQxDFkDYKhFdT1mINf6UvGbJXAVg6WgX_U5TGZ-Dbo_vlWhmEqc8vfEpdnm-RoiZo2RO3c=emb
> ]
>
> Phillip Kara, HTL | Senior Research Associate
>
> University of North Texas Health Science Center
>
> Division of Research and Innovation
>
> a: 3500 Camp Bowie Blvd, Fort Worth, TX 76107
>
> p: 918-281-9060
>
> w: http://www.unthsc.edu/corelabs
>
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Re: [Histonet] Fixing and processing mouse eyeballs

[Paula Keene Pierce via Histonet]

Hi,
I have done 10s of thousands of mice eyes, as well as other species, processed 
to paraffin in my career with no retinal detachment and enhanced IHC and ISH, 
but using my own method.

Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Thursday, October 12, 2023 at 01:43:39 PM CDT, Li, Nathan via Histonet 
 wrote:  
 
 The J.Pang paper article John Kieran referenced might not be do-able in your 
lab without glyoxal but they have another excellent article here with more 
routine techniques:  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585658/

Nathan Li, BS. HTL(ASCP)CM QIHC
Histology Manager
Tufts University
Tufts Comparative Medicine Services
Animal Histology Core
Ziskind 240
Boston, MA 02111
(617)636-8359
Preferred pronouns:  he/him/his
http://sites.tufts.edu/histopath/
Please email any histology related messages to histoc...@elist.tufts.edu
https://form.jotform.co/tuftscms/histocore


“CLICK HERE – CMS EMPLOYEE RECOGNITION FORM – PLEASE HELP US RECOGNIZE CMS 
STAFF FOR THEIR CONTRIBUTIONS, BIG AND SMALL!”


-Original Message-
From: Charles Riley  
Sent: Wednesday, October 11, 2023 5:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fixing and processing mouse eyeballs

Does anyone have any helpful tips or protocols for fixing and processing 
schedules for adult mouse eyes?

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Re: [Histonet] Small Inexpensive Stainer for Special Stains

[Paula Keene Pierce via Histonet]

Just a thought, but H stainers have multiple programs to code.
Having a second machine to use for specials would be an option.
Maybe even bring slides to water and use different rows of containers for 
different stains.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Thursday, September 14, 2023 at 09:52:10 AM CDT, Patricia Latham via 
Histonet  wrote:  
 
 Thank you for asking - I am also looking for recommendations on a small
stainer.
Pat Latham

On Thu, Sep 14, 2023 at 9:58 AM Pairan, Kelly via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Good Morning,
> One of my sites is looking to automate a few of their specials stains.
> They do not have a high throughput and are looking for something small,
> reliable and budget friendly.They are not looking for something as high
> tech as an Artisan or a Benchmark at this time.  They are looking to
> automate Masson's Trichrome, Giemsa, PAS-F and Iron right now.  Does anyone
> know of an instrument that meets this criteria?
>
> Thanks,
> Kelly
>
> Kelly Pairan, HT(ASCP)CM, HQIPCM
> Technical Scientist Anatomic Pathology and Cytology
> OhioHealth Laboratory Systems
> Suite 210 North Medical Building
> 3535 Olentangy River Rd
> Columbus, OH 43214
>
> Email: kelly.pai...@ohiohealth.com
> Work: (614) 566-3575
> Cell: (614) 312-0104
>
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Re: [Histonet] Alcian blue van gieson double stain

[Paula Keene Pierce via Histonet]

Hi,
if they are wanting to stain elastic, Alcian blue, and Van Gieson, I recommend 
doing the Verhoff elastic stain first, then the Alcian blue, and then the Van 
Gieson.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Thursday, March 9, 2023 at 01:05:14 PM CST, Charles Riley via Histonet 
 wrote:  
 
 I am working with a researcher and they saw an article where someone
performed an Alcian Blue  and elastic van Gieson double stain.  If anyone
has done this before can you provide your protocol.  I am trying to figure
out the best way to perform this process to get the best staining results.
Here are my questions/thoughts

1. Is it better to run the Alcian blue as usual and then perform the van
gieson stain?
2. Will the differentiating solution in the van gieson stain affect the
alcian blue staining in any way?
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Re: [Histonet] Goat Antibodies

[Paula Keene Pierce via Histonet]

Hi,
Vector Labs has Goat polymer ImmPress detection kits in both alkaline 
phosphatase and HRP.
I find any polymer detection greatly reduces background.


Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Monday, August 16, 2021, 04:17:10 PM CDT, Rebecca E. Ashley via Histonet 
 wrote:  
 
 I'm just curious what detection kits everyone is using for their goat 
antibodies.  We're getting a lot of background that we haven't been able to 
decrease significantly so I'm wondering if different reagents would help.

Thanks,
Rebecca

Rebecca Ashley, HTL(ASCP)CM
Laboratory Technician III
Histopathology
Wyoming State Veterinary Laboratory
1174 Snowy Range Rd.
Laramie, WY 82070
307-766-9946
Pronouns: she | her | hers (what's 
this?)


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Re: [Histonet] IF with permanent mounting media?

[Paula Keene Pierce via Histonet]

Hi,
you can use "Crystal Mount" first, let it dry, and then coverslip with 
xylene/resin.
I do this routinely with AEC IHC and oil red O and other fat stains.
Crystal Mount only on retinal flat mounts to be shipped out to others for 
future staining, as it can be removed with water,

FYI: Crystal mount is no longer made under that name, but do a search on 
Electron Microscopy Sciences website and their version will come up in the 
results.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Friday, May 28, 2021, 10:43:39 AM CDT, Morken, Timothy via Histonet 
 wrote:  
 
 Has anyone tried using xylene/permanent mounting media for immunofluorescence 
stains? I had a question from a pathologist who wondered if we could do this. I 
have never heard of anyone doing it.

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

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Re: [Histonet] Tissue Orientation Markers

[Paula Keene Pierce via Histonet]

You could use pieces of fixed/processed plant leaf embedded on edge.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Friday, March 26, 2021, 01:23:39 PM CDT, Igor Deyneko via Histonet 
 wrote:  
 
 Hello fellow Histonetters,

Has anyone ever worked with any type of a tissue orientation marker that is
not a dye, but rather some sort of a synthetic product that can be
processed and then sliced in a microtome?

Previously, we have used plain gut sutures and dyed histogel, which we
processed and made paraffin punch biopsies and then placed it as a tissue
marker along with tissues of interest and processed again. It's quite
cumbersome, so I'm trying to see if there are better options out there?

Thank you!

Igor Deyneko
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Re: [Histonet] Antibody diluent

[Paula Keene Pierce via Histonet]

I just purchased antibody diluent from Electron Microscopy Sciences.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Tuesday, March 23, 2021, 01:36:12 PM CDT, Morken, Timothy via Histonet 
 wrote:  
 
 Maria, we went back to using Dako Diluent, which we had just switched away 
from to save money!

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

-Original Message-
From: Maria Cruz via Histonet  
Sent: Tuesday, March 23, 2021 10:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Antibody diluent

Like other folks in the histo community, I’ve recently experienced the 
inability to buy diluent from Leica.  Now wondering what others are doing to 
handle this problem.  TIA!
Maria
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Re: [Histonet] Old Tissue Tek processor

[Paula Keene Pierce via Histonet]

There is one for sale on ebay.
https://www.ebay.com/itm/FISHER-HISTOMATIC-TISSUE-PROCESSOR-MODEL-166-MP/151784782912?hash=item235713fc40:g:CbkAAOSwgQ9V02Xl




Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Tuesday, October 13, 2020, 01:33:49 PM CDT, Brett Connolly via Histonet 
 wrote:  
 
 Does anyone have a pic of the old style Fisher Tissue Tek Processor?...the one 
that was popular in the early 1980s. I couldn’t find any on-line. Thanks.

Brett Connolly, Phd, HTL(ASCP)

Sent from Mail for Windows 10

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Re: [Histonet] Repairing a damaged TMA block

[Paula Keene Pierce via Histonet]

Hi,
align the block as well as you can in its corresponding size mold and place in 
a 37C oven for about 30 minutes to an hour.
This will soften the block enough that you can use a straight edge to gently 
realign the warped side.
You may need to repeat a couple of times to not force it.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Friday, September 4, 2020, 12:20:45 PM CDT, Kristyn Ferber via Histonet 
 wrote:  
 
 Good afternoon Histonet!

Does anyone have any experience repairing a precious TMA block that was damaged 
by heat? Part of the block is okay but half was exposed to heat and experienced 
some melting which misaligned some cores and has the block on a slant. We know 
that this has probably happened to someone else with all the bad storage 
practices of the past and damage to blocks in the mail.

Thanks in advance for any advice you have!

Best,

Kristyn

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Re: [Histonet] Update info. on old topic?

[Paula Keene Pierce via Histonet]

I am interested in this too, with my main question being the quality of digital 
images from film coverslipped slides.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Wednesday, August 5, 2020, 07:57:05 AM CDT, Sanders, Jeanine 
(CDC/DDID/NCEZID/DHCPP) via Histonet  wrote: 
 
 
 Good morning!

I need to provide some information to my team about film vs. glass 
coverslipping. I have a lot of positive info. regarding film but are any cons? 
And I need to come up wit the pros of glass over film. Anyone with extended 
experience have some current information for me?

Thanks very much,

Jeanine Sanders, BS, HT(ASCP), QIHCCM(ASCP)
Centers for Diseases Control and Prevention
1600 Clifton Road NE
MS H18-SB
Bldg. 18, Rm SB-114
Atlanta, GA 30329
404-639-3590


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Re: [Histonet] Crystals forming in Hematoxylin

[Paula Keene Pierce via Histonet]

That is the aluminum salt precipitating out.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Thursday, July 9, 2020, 11:47:53 AM CDT, Terri Braud via Histonet 
 wrote:  
 
 Hello Histo Peeps - What are the clear crystals that form in Hematoxylin?  We 
use Richard-Allen 7211.  We love the stain, but occasionally, when a bottle has 
been previously opened, we find clear crystalline precipitate forming in the 
bottom.  Today, we had a huge one (over an 2" in greatest diameter) that was 
quite beautiful.  It had a few occlusions of purple streaks, but otherwise was 
clear.  Doesn't seem to affect the stain, so I was just curious.
Inquisitively yours, Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

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Re: [Histonet] Anyone having difficulty with tissue adhesion?

[Paula Keene Pierce via Histonet]

Hello, 99% of my work are eyes and they are notorious for not adhering well.
One thing I have found is that white glass slides work the best and cannot use 
any green glass.
I would like to try your slides.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Tuesday, June 16, 2020, 03:01:41 PM CDT, Ken M via Histonet 
 wrote:  
 
 Hi everyone

We have developed a new hydrophilic IHC microscope slide that uses a new 
adhesive process that dries up to 50% faster than a normal Silane coated 
charged slide and we are looking for someone that might be using a Ventana or 
who may be dealing with difficult tissue or a difficult protocol that may be 
causing them to lose tissue who can try them for us in a difficult real world 
setting. If Anyone is interested, I can send you several packs at no charge.

Ken
kdea...@hotmail.com

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Re: [Histonet] Shandon-Cryotome-Fse battery change ?

[Paula Keene Pierce via Histonet]

Yes, it is the same as the battery on a computer tower motherboard to keep the 
clock going.
Turn off. Unplug. Remove panel.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Wednesday, May 27, 2020, 07:32:56 AM CDT, richard.wild--- via Histonet 
 wrote:  
 
 Hi

Is it possible to change battery that keeps usual data (language etc) on 
Shandon-Cryotome-Fse*?* And where is it ?

Best

Richard
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Re: [Histonet] UV Light in Fume Hood

[Paula Keene Pierce via Histonet]

It will need to be UVC or UVD.
UV light works by scrabbling the DNA in bacteria, viruses, and mold. But I will 
also scrabble yours too.
Hospitals, airports, and other businesses are starting to use UVD robots to 
disinfect patient rooms and large areas with no people present at the time.
UV light can be an added way to disinfect your hood, but get one with a timer 
and don't turn it on until you are ready to leave for the day and no 
housekeeping personnel will be in the lab during its use.
Practically all of my work is shipped in and I have a UVC light in a closet and 
zap packages so I can open them sooner.
Some household air cleaners also have UV light incorporated into their system. 
OION Technologies is one brand. No commercial stake, I am just an end user of 
it at home. 
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Thursday, May 14, 2020, 01:33:36 PM CDT, Paula via Histonet 
 wrote:  
 
 Hello everyone.. I hope all is well.

 

My admin wants me to look into possibly adding a UV light inside our fume
hood, which currently does not have one.  I'm thinking maybe it's not as
effective to kill viruses as we think it would be.

 

If anyone can share your thoughts about it, or if anyone has bought one and
has any insights to share, that would be greatly appreciated.

 

Have a great day and take care,

Paula

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Re: [Histonet] IHC DAB

[Paula Keene Pierce via Histonet]

Since you are newbies, I am just putting IHC 101 out there. 
Permanent red is for alkaline phosphatase detection and DAB is for HRP.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Friday, May 1, 2020, 10:29:00 AM CDT, Kristy Castillo via Histonet 
 wrote:  
 
 Hi Histonetters,

We are starting our IHC (fun times), we are having trouble with the DAB
lighting up.  Processed for 5 thru 10 minutes and still nothing.  Our
permanent red is working just fine.  Any ideas.

Thank you!

Kristy
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Re: [Histonet] Histonet Digest, Vol 197, Issue 23 recovering RNA from FFPE

[Paula Keene Pierce via Histonet]

Biochain too.
Paula
 

On Saturday, April 25, 2020, 01:08:53 PM CDT, Steve McClain via Histonet 
 wrote:  
 
 1) Does anyone know of a method of recovering RNA from FFPE?
2) Does anyone know a lab that has done it?

Thanks,
Steve A. McClain, MD
McClain Labs
45 Manor Road Smithtown, NY 11787
631 361-4000 cell 631 926-3655

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Re: [Histonet] Histonet Digest, Vol 197, Issue 23 recovering RNA from FFPE

[Paula Keene Pierce via Histonet]

By doing an internet search for FFPE PCR Platform, Thermo, Agilent, Biogen, 
Qiagen, etc. all have this.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Saturday, April 25, 2020, 01:08:53 PM CDT, Steve McClain via Histonet 
 wrote:  
 
 1) Does anyone know of a method of recovering RNA from FFPE?
2) Does anyone know a lab that has done it?

Thanks,
Steve A. McClain, MD
McClain Labs
45 Manor Road Smithtown, NY 11787
631 361-4000 cell 631 926-3655

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Re: [Histonet] Recommended thickness of Amyloid sections

[Paula Keene Pierce via Histonet]

As this is my 41 year of being a registered HT, I was taught that slides for 
amyloid are to be cut at 8-10µm.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Thursday, April 16, 2020, 03:18:44 PM CDT, Ken M via Histonet 
 wrote:  
 
 Hello All.  We have always cut all of our histology control slides at 5m.  We 
were told today that it is common practice to cut Amyloid at 8m?  Is this your 
experience?

Ken
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Re: [Histonet] Metal Testing

[Paula Keene Pierce via Histonet]

A lab with X-Ray diffraction on an SEM could help. Try the university electron 
microscopy labs.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Friday, April 3, 2020, 11:14:30 AM CDT, Gauch, Vicki via Histonet 
 wrote:  
 
 Hi everyone,
Does anyone know of a laboratory that can identify the type of metal in a 
foreign body ? I need a lab that is licensed to do testing in NY state.  I have 
already tried LabCorp, MAYO and NMS.  Any help would be greatly appreciated.

Thank you,
Vicki Gauch
AMCH Pathology
Albany, NY
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Re: [Histonet] EM as a "contract lab" lab for clia purposes

[Paula Keene Pierce via Histonet]

Yes, they would still need the certification, however as only the technical 
part. A pathologist will need to be designated the medical director and all 
pathologists reading the results will need to have their credentials added.
The medical director will need to also sign off on any complex procedures, ie. 
gross examination, done by the lab personnel.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Monday, March 30, 2020, 05:43:58 PM CDT, Morken, Timothy via Histonet 
 wrote:  
 
 Here's a question that came up. A stand-alone EM lab at a college that is 
associated with a medical center, but in a different city, wants to do the EM 
workup for the medical center. But they would only take in samples, do the EM 
processing and take images then the pathologist would read the images in the 
other city.

Does that lab need a CLIA certificate? Can they come under the existing 
hospital lab certificate (interpretation and signout is all done at the medical 
center in the other city) and be audited as contract lab?

My guess is they still need a certificate since they are handling human samples 
for diagnostics. But

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

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Re: [Histonet] Cutting room and social distancing

[Paula Keene Pierce via Histonet]

Yes, that is one of the CDCs' recommendations.
Use different shifts to spread out people.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Sunday, March 15, 2020, 01:23:45 PM CDT, Shannon Gower via Histonet 
 wrote:  
 
 Hello all,

In my lab,  the histology room is small with 4-5 people in close proximity to 
one another. Is anyone else considering modifying shifts to separate staff and 
reduce exposure in this crazy world of COVID? 
Trying to make the best plan while expecting every to change day to day. Any 
ideas on how to keep labs free of virus?

Any input is appreciated

Shannon Gower
Histology Manager
PathGroup Salem, VA




Sent from my iPhone
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Re: [Histonet] hard Tick histology

[Paula Keene Pierce via Histonet]

The exoskeletons of insects are made of chitin and contain little calcium.
Lysol spray contains a type of phenol which will soften chitin. The phenol is 
what kills bacteria, mold, and viruses by destroying their outer shells.
Try spraying Lysol in a small container so you have a few mls. It will not 
evaporate. Soak the tick in this for about 10-20 minutes.
Instead, you may have a bottle of phenol sitting around somewhere.
Or DIAPHANOL (a 50 per cent solution of glacial acetic acid saturated with 
chlorine dioxide) is also used, but can also damage he internal tissue.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Thursday, March 5, 2020, 03:26:47 PM CST, Mike Tighe via Histonet 
 wrote:  
 
 Hello,

I was wondering if someone might have some expertise on how to prepare a 
hard-tick for sectioning in paraffin. I have been decalcifying to soften the 
exoskeleton but not sure if that even helps. I have used acid decalcifying but 
would EDTA be more appropriate? I have punctured the ticks and used vacuum for 
all steps (fixation, decalcification, dehydration etc). Still crunchy on 
the outside and soft in the middle!

Any help would be greatly appreciated!!

Thanks!
Mike


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Re: [Histonet] alternative labels for zebra printer TLP 2742 connected to dako agilent autostainer plus

[Paula Keene Pierce via Histonet]

Hello,
I purchase my solvent resistant labels and thermal ribbons for my Zebra printer 
from Electronic Imaging Materials.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Friday, January 24, 2020, 01:12:38 AM CST, richard.wild--- via Histonet 
 wrote:  
 
 Hi the list.

Do you know alternativ labels for zebra printer TLP 2742, connected to 
dako agilent autostainer plus ? Did you try it ?

It should have the same size, and same distance between labels, and be 
resistant to solvant and other chemicals, and thermal printed

(instead of Agilent dako autostainer : S341730S3417Slide 
Label Kit, Large Flap, 6 rolls per kit, 3000 label)

Best

Richard


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Re: [Histonet] X-gal staining

[Paula Keene Pierce via Histonet]

I have done paraffin processing and sectioning of X Gal stained eyes and embryo 
heads.
The staining is done for the blue color first, post fixed, and then 
processed.Xylene should be avoided, as it is what removes the X Gal target, and 
a xylene substitute used such as StatLab's XS3.
Deparaffinization and dehydration for coverslipping should also be done with 
the xylene substitute.
I counterstain with Nuclear Fast Red.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Saturday, January 11, 2020, 01:22:43 PM CST, Hobbs, Carl via Histonet 
 wrote:  
 
 Hi
No replies so far so.my pennyworth.
Imho ...no
beta Gal enzyme is inactivated by std Pwax processing.
So, when you apply the X-gal substrate and chromogen solution to Pwax sections, 
 it will not give you a positive blue final reaction product
If you want to detect beta Gal in FFPWS and visualise with DAB,  you will have 
to use an anti beta Gal antibody.
Finding an anti beta Gal enzyme antibody, that works in FFPWS +/- AR is not so 
easy.

Good luck

Carl

Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge 
Kings College London
London
SE1 1UL
 

020 7848 6813






From: histonet-requ...@lists.utsouthwestern.edu 


Sent: 08 January 2020 18:00

To: histonet@lists.utsouthwestern.edu 

Subject: Histonet Digest, Vol 194, Issue 4
 

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Today's Topics:


  1. X-gal staining (Margaryan, Naira)

  2. (no subject) (Waitts, Celeste)



--


Message: 1

Date: Tue, 7 Jan 2020 18:21:31 +

From: "Margaryan, Naira" 

To: "'histonet-request (histonet@lists.utsouthwestern.edu)'"

        

Subject: [Histonet] X-gal staining

Message-ID:

        


        

Content-Type: text/plain; charset="us-ascii"


Hello histonetters,


I have request from a scientist to perform X-gal staining on the formalin fixed 
paraffin-embedded kidney.

I was wondering if there is any possibility to do this staining on FFPE tissue 
as well as if there any possibility to perform a peroxidase DAB  staining for 
the X-gal? If so, may I ask you for a detailed protocol to perform this 
staining.

Any input and explanation why it is impossible is appreciated.



Thanks in advance,

Naira




--


Message: 2

Date: Wed, 8 Jan 2020 16:46:18 +

From: "Waitts, Celeste" 

To: "'histonet@lists.utsouthwestern.edu'"

        

Subject: [Histonet] (no subject)

Message-ID:

        <131bf77daa5d4ba08f9076d9afbf1...@exchnode1.local.centrastate.com>

Content-Type: text/plain; charset="us-ascii"


Question      VIP6    SHORT BIOPSY RUN    USING CLEARITE

ANYTHING


Celeste Waitts

Histology Supervisor

Centrastate Medical Center

Freehold, NJ  07728

(732-303-5071)




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Subject: Digest Footer


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End of Histonet Digest, Vol 194, Issue 4



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Re: [Histonet] How do you section PPFE tissue in embedding rings?

[Paula Keene Pierce via Histonet]

Hi,
I used embedding rings back in the 80s and still use the chuck adapter for 
large blocks. I do eyes.
I have a couple John's Hopkins chuck adapters and could loan you one.

Send me a picture of your microtome with a closeup of the chuck adapter to see 
if it will fit.
Do you have an old AO 820 sitting around? I know it will fit it.
The old Leica 1512s had an adapter that would work too.
Otherwise, I suggest just re-embedding.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Thursday, September 5, 2019, 01:16:21 PM CDT, Preiszner, Johanna via 
Histonet  wrote:  
 
 Hi,

I received a lot of specimens embedded in plastic embedding rings...have a 
Leica RM2135 microtome.

How can I put these blocks in the clamps of the microtome?

Searched Internet with zero success.

Nobody in town ever used embedding rings so I can not borrrow an adapter if 
that's what I need.

Could anybody please help out?

Re-embedding the tissue in regular cassettes would cost the client a lot of 
money.


Thanks,

Johanna Preiszner
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Re: [Histonet] IHC mouse optomization

[Paula Keene Pierce via Histonet]

Vector Labs ImmPress kits rock for animal IHC.
https://vectorlabs.com/browse/immpress-polymer-detection-kits-for-ihc




Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953www.excaliburpathology.com 

On Wednesday, June 12, 2019, 10:13:48 AM CDT, Blanca Lopez via Histonet 
 wrote:  
 
 Morning,
I need a good advice in optimizing Mouse antibodies for research purposes. I 
use Dako/Agilent products. COX6B2 from Sigma is giving us hard times. Dako kit 
HRP has a mixture of mouse and rabbit so we think that might be the reason 
become negative.
Is there any tips for optimizing antibodies special for mouse tissue? Or if you 
can share your best procedures in IHC mouse stains. I can have a different ways 
and products to try. Any suggestion or opinions count. If you have any website 
that I can learn more about IHC for research mainly done in  Xenograft.
Thank you everybody:)

Blanca Lopez HT (ASCP)cm
Senior Histotechnologist
UT Southwestern Medical Center
Harold C. Simmons Comprehensive Cancer Center
UTSTR Biorepository Tissue Lab
6000 Harry Hines Blvd NB5.102
Dallas, Texas 75390
214-648-7598
blanca.lo...@utsouthwestern.edu





UT Southwestern


Medical Center



The future of medicine, today.

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Re: [Histonet] Looking for vendor for Zeus Wash solution

[Paula Keene Pierce via Histonet]

You can also use Michel's buffer.
Electron Microscopy Sciences has it.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953www.excaliburpathology.com 

On Thursday, March 21, 2019, 12:29:45 PM CDT, Martha Ward-Pathology via 
Histonet  wrote:  
 
 I am having trouble locating a distributor for the Zeus Wash solution 
(cat#103) that we use to wash our skin and renal biopsies.    Cardinal and 
Fisher do not seem to carry it and we do not have Zeus Scientific in our system 
as a vendor we can use.  Any help would be appreciated!

Thanks,

Martha Ward
Wake Forest Baptist Health
Winston-Salem, NC 27157
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Re: [Histonet] Roche "bashing"

[Paula Keene Pierce via Histonet]

Yep.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953www.excaliburpathology.com 

On Wednesday, March 6, 2019, 9:14:39 PM CST, P Sicurello via Histonet 
 wrote:  
 
 It's not Roche "bashing", these are messages come from concerned histology
professionals to inform the Histology world that there are issues with a
product from a certain vendor.  Born out of frustration with the vendor who
does nothing about the problem and suggests that the issue lies with the
user.  Despite the fact that these "users" have been using the products for
years, with or without issue, and they continue to use the product, because
when the machines work they work very well.

Now if this *were *bashing there would be comments like:  "Roche doesn't
care about patient's or how these test failures effect patient outcomes."
or  "Those in charge are nothing but a bunch of money grubbing scum suckers
who only care about the bottom line."

Perhaps, and this is a stretch, if one day, they or someone they love is
negatively impacted because the HER2 dispenser failed (and her breast
cancer diagnosis and treatment is delayed), or that a transplanted organ
fails because, once again, the special stain that indicated rejection
didn't work.  But who I am I kidding?  Sure there are lots of dedicated
people in the trenches working hard to create medicines or improve testing,
but they aren't the ones who are who don't care.

Roche is too large to care about the outcomes of their failed testing
products, we are small peanuts compared to the money they make off of
pharmaceuticals.  Last year the overall diagnostics section made 12,879
(CHF in millions) or $12,874 (US in millions) of which the tissue
diagnostics division contributed  1,112 (CHF in millions) or $1,102 (US in
millions). Compare that to their total sales of 56,846 (CHF in millions) or
$56,602 (US in millions).  Our little tissue diagnostics division
contributes *1.9%* of their total sales and about *8.6%* to the overall
diagnostics division.  It's all right there in Roche's annual report,
THAT'S why they ignore us.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

9300 Campus Point Drive

La Jolla, CA 92037
(P): 858-249-5610



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On Wed, Mar 6, 2019 at 3:56 PM Jennifer Saunders via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> The dispenser problem have been around long before Roche entered the
> picture. At least a problem since 1997.. Ventana usto be really good about
> replacing leaky dispensers back then. If they or Roche had ever taken the
> problem seriously enough to actually fix the problem nobody would be doing
> any Roche bashing!!!Jen Saunders HT (ASCP)Portland Oregon
>
>
> Sent from Yahoo Mail for iPhone
>
>
> On Wednesday, March 6, 2019, 8:18 AM, Brett Connolly via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
>
> As a retiree from big pharma I find your generalization to be ignorant and
> insulting. There are many, many researchers and laboratory personnel in big
> pharma that don’t fit your description, believe me!
>
> Brett Connolly, HTL(ASCP), PhD
>
> Sent from Mail for Windows 10
>
> From: Jeff Jurczak via Histonet
> Sent: Wednesday, March 6, 2019 10:58 AM
> To: Victoria Baker
> Cc: histonet@lists.utsouthwestern.edu; Terri Braud;
> kelley.jor...@roche.com
> Subject: Re: [Histonet] Roche "bashing"
>
>
> Like all employees at big pharma companies, Kelly has been indoctrinated
> into believing that her company, her products, and the service rendered is
> perfect. To believe otherwise is a quick way to find the door out. As a
> corollary, there can be no problems other than user error ones.
>
>
>
> Sent from myMail for iOS
>
>
> Wednesday, March 6, 2019, 7:03 AM -0600 from
> histonet@lists.utsouthwestern.edu  :
> >Kelly,
> >
> >It is not bashing, it is frustration and concern.
> >
> >We need our instruments and reagents to work so that we deliver reliable
> >and consistent results to our pathologists.  They trust our work and
> >dedication to them and ultimately the patient.  When something like this
> >happens and we can't get a real resolution it undermines many things, but
> >trust is first for me.
> >
> >Vikki
> >
> >On Tue, Mar 5, 2019, 10:44 AM Terri Braud via Histonet <
> >histonet@lists.utsouthwestern.edu > wrote:
> >
> >> Such a wonderful insight into the thought process of Roche.  Accused of
> >> bashing when I am telling the truth about failed 

Re: [Histonet] benchtop fume hoods

[Paula Keene Pierce via Histonet]

I use a Labconco. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur 
Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 
405-759-7513www.excaliburpathology.com

  From: Allan Wang via Histonet 
 To: 
Cc: "histonet@lists.utsouthwestern.edu" 
 Sent: Thursday, May 10, 2018 12:02 PM
 Subject: Re: [Histonet] benchtop fume hoods
   
I'd like to know as well.

Thanks,
Allan

On Thu, May 10, 2018 at 11:25 AM, Histology via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hi all,
>
> Can anyone share what type of benchtop fume hoods they are using in the
> lab?  This would be for manual coverslipping and grossing.
>
> Thanks in advance!
>
>
>
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Re: [Histonet] Backup Power Supply

[Paula Keene Pierce via Histonet]

Hi, 
this is one that can charge by being plugged in and is larger than ones for 
computers.
http://www.wisefoodstorage.com/humless-portable-power-storage-and-fuelless-generator.html

They also have larger ones.

I have no relationship with Wise, except being a customer. Paula Keene Pierce, 
BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, 
OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com

  From: Gareth Davis via Histonet 
 To: "Histonet@lists.utsouthwestern.edu"  
 Sent: Monday, September 25, 2017 3:18 PM
 Subject: [Histonet] Backup Power Supply
   
Hello All,
​We need back-up power supply via CAP regulations.  Besides a generator or
a dedicated supply through a hospital back-up generator, what are labs
using.  We are a small lab located in a clinic.  There is no back-up power
supply in the clinic.  Wondered if there was anything out there that could
be used, like a battery back-up.  My only concern is our processor, which
is run overnight.  But, we need something.
Thanks,
-- 
*Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm
Yuma Gastroenterology
Yuma, AZ 85364
928-248-5259
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Re: [Histonet] Need help identifying a product!!!

[Paula Keene Pierce via Histonet]

Have you tried MarketLab? They have things like that. Paula Keene Pierce, BS, 
HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 
73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com

  From: "Cooper, Brian via Histonet" 
 To: "histonet@lists.utsouthwestern.edu"  
 Sent: Friday, September 1, 2017 1:34 PM
 Subject: [Histonet] Need help identifying a product!!!
   
Happy Friday Histonet!

Our cassette printed died this week, and we've been handwriting cassettes for 
the last couple of days.  It's been YEARS since I had to do this; BOY has my 
handwriting atrophied since then!  I remember we used to have this little metal 
rack that held the cassettes perfectly stationary when you wrote on them; the 
rack held about 20-25 cassettes in 4 or 5 rows.  Do any of you remember these?  
I've been searching online for a couple days, but Google and all the lab supply 
sites just aren't catching what I'm looking for.  Our printer will be fixed in 
a few hours, but just in case, I'd like to have one of these racks for the 
future.  Any ideas?  Should I check antique shops?  :)

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357    Pager: 213-209-0184
bcoo...@chla.usc.edu


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[Histonet] Butterfly Histology

[Paula Keene Pierce via Histonet]

Hi,
I do drosophila and other insects.
Use an alcoholic fixative such as Davidson's for better infiltration. Transfer 
to 70% EtOH after 24-48 hours.
Normal processing schedule with 30 minutes to an hour starting at 70-80%. 1-1.5 
hours each for paraffin. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur 
Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 
405-759-7513www.excaliburpathology.com
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Re: [Histonet] No Ribbon

[Paula Keene Pierce via Histonet]

Hi,
the blades are packed with a tiny, bit of oil. This can cause you to not obtain 
a ribbon.
Try gently cleaning with xylene, followed by alcohol before sectioning. Paula 
Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake 
DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com

  From: Gareth Davis via Histonet 
 To: "Histonet@lists.utsouthwestern.edu"  
 Sent: Tuesday, April 11, 2017 11:55 AM
 Subject: [Histonet] No Ribbon
   
I feel like I should be able to figure this one out, but I have been having
trouble with getting a ribbon for a while now.  I have a Leica Microtome
RM2125 and I use either a AccuEdge blade (I get one block to cut then it
starts to compress) or a StatCut blade (sections do not stick together at
all), both High Profile.  The angle is about 4 microns.  I change the angle
a lot to see if that makes a difference and it doesn't.  There are so many
factors that could be the issue, I think lack of humidity may be the main
one. So, suggestions please!!

-- 
Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm
Yuma Gastroenterology
Yuma, AZ 85364
928-248-5259
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Re: [Histonet] Tissue Fixation

[Paula Keene Pierce via Histonet]

Bravo Rene! Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, 
Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 
405-759-7513www.excaliburpathology.com

  From: Rene J Buesa via Histonet 
 To: T H ; "histonet@lists.utsouthwestern.edu" 
 
 Sent: Friday, March 31, 2017 8:44 AM
 Subject: Re: [Histonet] Tissue Fixation
   
What you describe as a possible scenario is absolutely possible.If your PT does 
not "want to hear" about it, suggest she gets a "hearing aid" or to study 
something about histotechnology or even better yet, pay attention to what a 
professional on the subject (you) has to say about it. You would never dare to 
question her diagnosis, why would she question yours on this subject?René 

    On Friday, March 31, 2017 9:11 AM, T H via Histonet 
 wrote:
 

 Good Morning,


I have a pathologist that is not happy with the fixation on some of our LEEP 
specimens.  She swears its histology doing something to the specimen to cause 
the tissue to look unfixed on only "part" of the LEEP specimens (all the same 
client specimens).  She claims we must be diluting our formalin to cause this 
issue or "something".  We mentioned maybe it was on the clients end not placing 
them in 10% formalin right away, she wouldn't hear of it.


Let me give you some back ground on how our process works.  Our clients send us 
all our specimens to us via Overnight FedEx or UPS in 10% formalin they will 
then they sit in 10% formalin in-house until the processors are started around 
3pm and sits an additional 5 hours in 10% formalin on the processor before the 
processor actually starts.  That being said the fixation process has had a 
pretty good start before we ever even touch it.


My question is, and I thought I had read this in the past is, when a specimen 
is left out prior to fixation and lying on a absorbent surface such as a paper 
towel, won't the area of the tissue touching the absorbent surface begin the 
disintegration processes faster in that exact area then the rest of the 
specimen?  Or if you have any other suggestion on what might be happening to 
only "certain" specimens would be great as well.


Thanks for your help!


Tim

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Re: [Histonet] malarial pigment

[Paula Keene Pierce via Histonet]

Lee Luna's third edition.
Page 43, Method II
1. Deparaffinize and hydrate to water.2. Wash in distilled water.3. Immerse 
slides in 100 ml of 70% alcohol, to which has been added 2 ml of 28% ammonium 
hydroxide, for 3 hours.4. Rinse in water.5. Rinse in 1% glacial acetic acid.6. 
Wash well in distilled water and stain as desired. Paula Keene Pierce, BS, 
HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 
73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com 

On Tuesday, March 7, 2017 9:46 AM, "Sanders, Jeanine (CDC/OID/NCEZID) via 
Histonet"  wrote:
 

 Good morning!

Does anyone know of a method using ammonium hydroxide to remove malarial 
pigment from tissue sections?

Thanks!


Jeanine H. Sanders
Infectious Diseases Pathology Branch
Centers for Disease Control and Prevention
1600 Clifton Rd., NE MS-G32
Atlanta, GA 30329


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Re: [Histonet] aniline oil/Holzer stain

[Paula Keene Pierce via Histonet]

You could try adding aqueous NaOH slowly to what you have until you start 
getting tiny white droplets in the water. Then they will fall and form an oily 
layer below the water.
Decant the water or use a separator funnel to drain the aniline oil out the 
bottom.

 Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 
405-759-7513www.excaliburpathology.com

  From: Susan Bachus via Histonet 
 To: histonet@lists.utsouthwestern.edu 
 Sent: Friday, October 21, 2016 5:28 PM
 Subject: [Histonet] aniline oil/Holzer stain
   
I'm trying to locate aniline oil for the Holzer glial fiber stain.  I 
purchased what was listed as aniline oil from ENG, but the first hint that 
something was wrong was that it was incompatible with the chloroform also 
included in the differentiating solution.  Looking at the fine print on the 
bottle, I saw that it was actually "aniline oil, 2.5% aqueous", which I 
would have interpreted as only containing 2.5% water, except that it seemed 
to be completely aqueous (2.5% aniline?), inasmuch as it wouldn't mix with 
the chloroform.  I can't find any other source online.  I see that it is 
also called aminobenzine or phenylamine, but am also having trouble locating 
sources under those names.  I realize aniline oil is relatively toxic, but 
am using it under a fume hood.  I tried dipping slides separately in the 
(apparently aqueous) aniline and then chloroform, but still didn't get good 
differentiation.  Can anyone suggest a source or a substitute?  Many thanks 
for your help!  Susan 


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[Histonet] Pan Cytokeratin

[Paula Keene Pierce via Histonet]

Hello,
would anyone be willing to donate a recently expired, about to be tossed, vial 
of pan cytokeratin for a research project?
I will pay shipping.
Thank you, Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, 
Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 
405-759-7513www.excaliburpathology.com
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Re: [Histonet] Frosted slides

[Paula Keene Pierce via Histonet]

Hi,
they are called Dakin slides. 
A quick search found: 
http://www.emsdiasum.com/microscopy/products/preparation/slides.aspx
 Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 
405-759-7513www.excaliburpathology.com

  From: "Younes, Pamela S via Histonet" 
 To: "histonet@lists.utsouthwestern.edu"  
 Sent: Friday, July 8, 2016 12:06 PM
 Subject: [Histonet] Frosted slides
   
Hello,
We are looking for completely frosted slides to use for cytopathology.  Does 
anyone know a vendor?  We have heard that some labs with purchase clear slides, 
and sand-blast them, but that sounds like a difficult process.

Many thanks,
Pam Younes
Pamela Younes MHS, HTL(ASCP), CPC, PA(ASCP)
Pathologists’ Assistant,
Assistant Professor
University of Texas Health Science Center Houston, McGovern Campus
Department of Pathology and Laboratory Medicine,
6431 Fannin, MSB 2233A,
Houston, TX 77030

pamela.s.you...@uth.tmc.edu


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Re: [Histonet] Shandon Varistain Gemini Slide Stainer

[Paula Keene Pierce via Histonet]

This is so true!
I had a Fisher Histomatic automated slide stainer that was manufactured in 1985 
that I used until the building took a direct lightning strike JUST LAST YEAR!
My old VIP is still going!
 Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 
405-759-7513www.excaliburpathology.com

  From: Teri Johnson via Histonet 
 To: "histonet@lists.utsouthwestern.edu"  
 Sent: Thursday, June 23, 2016 2:04 PM
 Subject: Re: [Histonet] Shandon Varistain Gemini Slide Stainer
   
Hi Thomas,

Are you looking for true life expectancy or what is reported for depreciation? 
In my experience, most tissue processors never die, but only need to be retired 
due to lack of available support/parts or because a lab requires newer 
technology.

Also in my experience, a "better" model comes out soon after I have purchased 
one. So the life span is probably 20 years after you wished you had a different 
one. :-)

Best wishes,

Teri

Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA  92008
USA

Phone +1 760 516 5954
tejohn...@genoptix.com
www.genoptix.com

--

Hi All,
Does anyone know the life expectancy of the Gemini H stainer? My boss has 
asked me this question because we are in the process of requesting for a new 
one.

Thomas

Thomas Huynh  BS, HT (ASCP)
Histology Lab Supervisor |Department of Pathology HARRISHEALTH SYSTEM
5656 Kelly Street
Houston, Tx 77026
Lyndon B. Johnson General Hospital (LBJGH)
O: 713.566.5282 | F: 713.566.5285 | P: 713.297.1606 | 
thomas.hu...@harrishealth.org

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Re: [Histonet] floor vibration

[Paula Keene Pierce via Histonet]

Refuse the space and ask to be moved somewhere else.
Years ago our EM scopes were on the fourth floor and lines in photos taken 
could be seen from the vibration from people simply walking down the halls.
Finally moved the scopes to the basement on a section of concrete cut out 
completely separate from the rest of the building. Paula Keene Pierce, BS, 
HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 
73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com

  From: Nancy Schmitt via Histonet 
 To: "'histonet@lists.utsouthwestern.edu'"  
 Sent: Monday, May 16, 2016 10:03 AM
 Subject: [Histonet] floor vibration
   
Happy Monday!
We are moving to a new space and part of our area is above the laundry - there 
is some vibration from there.  Does anyone have any experience with this and 
could you please share how you accommodated this?  Special flooring, pads, etc.
Thank you much!

Nancy Schmitt HT, MLT (ASCP)

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[Histonet] Job Opening in CA

[Paula Keene Pierce via Histonet]

| 
|  |

 |


| 
| Dear Friends & Family,I am seeking a Research Scientist I, Stem Cell Biology 
for a stem cell therapy project that we just started. The funding has been 
secured for the entire project. The position is available immediately and the 
description is shown below. If you know somebody who may be interested in this 
opening, please share this e-mail with your contact. Any help, advice or 
recommendations are highly appreciated as always!Respectfully,Nikolay Turovets, 
Ph.D.CEO MediCell Technologies, LLC |

 |


| 
| 
| 
|  |

 |

 |

 |


| 
| RESEARCH SCIENTIST I, Stem Cell Biology |

 |


| 
| Job Description
Novel exclusive stem cell therapy project is seeking a highly motivated 
scientist to join its team.In consortium with a leading hospital in Los Angeles 
and a recognized business partner, MediCell Technologies is developing a novel 
stem cell-based therapeutic platform. The technology has been validated in 
animal proof-of-concept studies and attracts interests of key opinion leaders 
of the field. The funding has been secured for entire project.We are looking 
for a highly motivated and pro-active individual who is willing to grow with 
the project and contribute to the development of stem cell-based therapies. Our 
project is an outstanding opportunity for the right individual to display their 
talents and establish themselves in this highly competitive field.The scientist 
will be responsible for the derivation, expansion and characterization of a 
unique type of human stem cells. He or She will be involved in all range of 
activities associated with the technology transfer to a GMP facility as well as 
preparing a portfolio of Standard Operation Procedures. The Scientist will play 
an integral role in managing various aspects of lab operations-associated 
activities. The work will require periodic work on weekends, holidays and after 
business-hours time.This position requires hard work, ingenuity, and a strong 
commitment to a productive work environment. If you are not a highly confident, 
self-starting individual, this position is not meant for you.
 |

 |


| 
| Required qualifications   
   - PhD in cell biology, stem cell biology, tissue engineering or related 
discipline. 3+ years of work experience in stem cell research.
   - Outstanding hands-on techniques in stem cell culture, cell-based assays 
(including ICC, qPCR, FACS), and cell culture process development.
   - Excellent English written and oral communication skills, able to write 
SOPs, technical reports and protocols.
   - Demonstrated excellence in planning, executing, and analyzing experiments.
   - Able to troubleshoot, solve difficult problems, and develop process 
improvements.
   - Attention to detail and careful record-keeping.
   - Excellent organizational skills and ability to manage multiple projects.
   - Flexible team player excited to collaborate with internal and external 
partners.
   - Highly self-motivated.
   - Flexibility in working schedule to accommodate weekend/holiday and 
out-business hours work if necessary.
   - Authorized to legally work in US.
Preferred (but not required) qualifications:   
   - Experience with human pluripotent and/or mesenchymal stem cells is 
preferred.
   - Experience to work in GMP and/or GLP environment is appreciated.

Salary: up to $85,000/annual + health insuranceJob Location: Carlsbad, 
CARe-location expense: Not offeredEmployment term: Full time, 3 month probation 
period |

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| HOW TO APPLYPlease send CV and Cover Letter to Nikolay Turovets, PhD: 
n...@medicelltech.com |

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 Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 
405-759-7513www.excaliburpathology.com
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