I, personally, think EDTA SUCKS. a) it takes too long b) its not safe -
http://en.wikipedia.org/wiki/EDTA#Toxicity_and_environmental_considerations c)
its to old-fashioned.
Me, I'm a big proponent of Formic Acid decal. Holla off-list if you want me to
hit you up with a protocol, yo.
I suggest to start a board/forum. Really.
On Wed, 1 Apr 2009 23:13:09 -0700 (PDT), aa aa lactose.intoler...@yahoo.com
wrote:
Dear Histotech Experts!
I am working on a very complicated project, and could really use some
advice from both the guru-level histologists and also from the
What I got was a Rick Roll music video on Youtube. If aa aa is a
spammer, this was a slick way to attract our attention. Anybody else?
aa aa wrote:
Dear Histotech Experts!
I am working on a very complicated project, and could really use some advice
from both the guru-level histologists and
Mark,
Histology, who cares, turned up the volume and danced round the
office singing along to this Stock, Aitken and Waterman classic. This soul
boy even has the album on his iPhone.
Ian.
Dr. Ian Montgomery,
Histotechnology,
I.B.L.S. Support Unit,
Thomson Building,
University of Glasgow,
I think it was April fool...
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Ray
Sent: Thursday, April 02, 2009 5:10 AM
To: lactose.intoler...@yahoo.com
Cc: histonet@lists.utsouthwestern.edu
Subject:
KIDS, KIDS!!! Enough is enough. Take it off the list and respond to one
another privately. It's fast becoming like observing a daycare here.
Glen Dawson
IHC Manager
Milwaukee, WI
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Take it off the list and respond to one another privately.
Says he on the list and not privately
From: Dawson, Glen gdaw...@dynacaremilwaukee.com
To: Histonet histonet@lists.utsouthwestern.edu
Sent: Thursday, April 2, 2009 8:52:28 AM
Subject: RE:
It is getting difficult to remember when an item that actually dealt
with a histotechnology issue was posted.
This is how worthwhile discussion groups fail, endless prattle followed
by endless prattle about the endless prattle.
Geoff
--
**
Geoff
Looked like a scam to me so I hit Delete immediately.
No real name, no real address = scam
Geoff
Mark Ray wrote:
What I got was a Rick Roll music video on Youtube. If aa aa is a
spammer, this was a slick way to attract our attention. Anybody else?
aa aa wrote:
Dear Histotech Experts!
I
Does anyone know where you can purchase an Fc receptor blocker for
staph? Vendors welcome!
Thanks!
Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA 30333
(404) 639-3590
It is getting difficult to remember when an item that actually dealt with a
histotechnology issue was posted.
This is how worthwhile discussion groups fail, endless prattle followed by
endless prattle about the endless prattle.
And you plan to remedy this by posting more prattle about the
Specially when one contributor tries to outsmart or outwit another or have
the final saying.
René J.
--- On Thu, 4/2/09, Geoff McAuliffe mcaul...@umdnj.edu wrote:
From: Geoff McAuliffe mcaul...@umdnj.edu
Subject: [Histonet] The end of Histonet
To: Bernie Taupin bernietau...@ymail.com
Cc:
Looked like a scam to me so I hit Delete immediately.
No real name, no real address = scam
It was a link to a video, I believe, not a scam.
From: Geoff McAuliffe mcaul...@umdnj.edu
To: Mark Ray darkd...@comcast.net
Cc: histonet@lists.utsouthwestern.edu
Sent:
It wasn't funky it was a good video - but it had no relevance to what
we are supposed to be doing here on histonet. Now if somebody could
post the link to some Adam Lambert tunes...
Andi;-)
On Apr 2, 2009, at 2:10 AM, Mark Ray wrote:
What I got was a Rick Roll music video on Youtube.
Can someone explain to me the mechanism by which glutaraldehyde would
disrupt the blood brain barrier if the brain had not been flushed prior to
fixation?
Boma.
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Greetings,
I have been cutting sections of whole bat genitalia embedded in JB-4. My
intention is to study and photograph these sections in light microscopy. The
quality of the sections is excellent except for one detail. The medium
separates from the epidermis (but not any other tissue
Trumps Fixative
Ten ml of 40% formaldehyde and 2 ml of 50% glutaraldehyde were added to
200-mOsm buffer.
This solution could be conveniently prepared for routine use as follows:
1.16 g of NaH[2]PO[4] is added to 88 ml water and dissolved.
Add 0.27 g NaOH followed by the formaldehyde and the
Greetings,
I have a project sectioning whole bat genital organs. The study, in part,
involves measurement and so to avoid shrinkage I am using JB-4 and cutting with
a glass knife. The tissue comes out very well except for one problem. The
epidermis pulls away from the medium. I have
FREE TBS Tissue Processor (you pay shipping). Kind of a lemon.
Liesl Frasier
719-530-2200 x2195
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Do any of you know if this colored paraffin by Polysciences, Inc.
stains the tissue and if so would it auto fluoresce?
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cell Biology and Anatomy
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724
Hi Boma:
Whether or not a saline or buffer flush was used before glut. fixation
is of little consequence to the effects of glut as a fixative.The
saline/buffer washout is just to remove blood. In my experience most
people overdo the washout.
I am curious as to what disrupt the blood-brain
Histonet members:
As the list moderator, I now feel the need to ask everyone to please get back
to the business of histology and related topics. Histonet is run using the
University of Texas Southwestern Medical Center's resources and I suspect they
would not look favorably on large volumes of
Come on people. Yesterday was April Fool's Day and Histonet got Rick Rolled.
Today is the 2nd, move on.
PKP
From: Andrea Grantham algra...@email.arizona.edu
To: HISTONET histonet@lists.utsouthwestern.edu
Sent: Thursday, April 2, 2009 9:33:06 AM
Subject: Re:
Might I suggest that what we need on Histonet is to deal with some basic
questions in histology that have never been answered in detail but have only
been touched upon.
Histonet has a great deal of expertise and it seems we should be asking these
major questions.
Topics such as what is optimal
Dear histonetters,
I am in need of some help. Is it possible to stain a mouse mammary
gland in Carmine Alum after it has been fixed in 4% PFA? We originally
were fixing them in Carnoy's and then staining, but then it was decided
not to stain anymore and the glands were just fixed with the
I am looking for a protocol for embedding cell pellets in paraffin.
Thanks,
Louise
Louise Hartson, BA
Senior Technical Associate
University of Rochester
louise_hart...@urmc.rochester.edu
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We use this paraffin and it does not cause any permanent color change to
the tissue. When it is removed from the processor after infiltration,
the tissue does have some color, but after deparaffinization during
staining, there is no color left.
Hope this helps.
Emily M. Lockman, HT (ASCP)
Hello,
I really have some real links to real pictures of real relevance of the
real histonet list. Really promised.
http://img12.imageshack.us/img12/8513/ts0402162049.jpg
http://img13.imageshack.us/img13/6514/ts0402162104.jpg
So, has ever anyone experienced sth. like this?
My conjugate
I'm not in total disagreement with Barry. The FAQ is a good place to
start, and it might be all we need, but I wonder it shouldn't be
revisited just to be sure. There seems to be a lot of questions lately
on some of the basics as well as queries about instrumentation.
My question would be this:
Histogel works well for this purpose too.
--Original Message--
From: Thomas Pier
Sender: histonet-boun...@lists.utsouthwestern.edu
To: histonet@lists.utsouthwestern.edu
To: louise_hart...@urmc.rochester.edu
Sent: Apr 2, 2009 12:17 PM
Subject: Re: [Histonet] Embedding cell pellets
Fix the
True true that would be cool. But I don't mind repeated topics being discussed
again and again - it isn't always a bad thing, as although histology is a very
old and largely well-established science, there is room for improvements and
modifications over time.so I appreciate the ongoing
Hi everyone,
What brand low profile blades does everyone prefer and where do you order them?
Also, what blade angle does everyone prefer for cryosectioning?
Thanks
Stacey
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Boy Bernie,
See if I ever buy your albums again!
;-)
Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF)
Core Research Imaging Center
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397
--- On Thu, 4/2/09, Bernie Taupin
Hi all,
What exactly is it supposed to do when the switch Process mode (start
program-drain-purge) switch is set to purge?
I aquired this one allmost for free and I will use it - once it's up and
running - in the first place for plant anatomy.
Yep: it's some kind of a museum around here.
AccuEdge by Sakura Finetek are the best (avail. through VWR)
Not only have I tried a few other brands, but our former knowledgeable and
experienced histotech here at UB recommends AccuEdge.
Merced
--On Thursday, April 02, 2009 9:55 AM -0700 Stacey Barrick
barricksta...@yahoo.com wrote:
Try histogel from Richard Allan- available from Fisher.Easier than making up
agarose!
Bernice
Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
-Original Message-
From:
It's all I ever use and I've tried a bunch.
Bernice
Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Does anyone use GemCut parafin, and what do you think about it, any good, any
concerns?
Steve
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Recently we are experiencing random blocks of tissue (approx. 3 blocks out of
400/2x) that appear to be burned.? The tissue is prostate and the staining
looks dark.? Upon recutting, sometimes it is better, sometimes it is not, which
negates my ability to rule out the stainer completely.
We
We use MX premier microtome blades and order from ThermoFisher. I do have
the accuedge blades but prefer the MX blades. The accuedge blades just don't
seem to be as good as they used to be...my opinion only.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's
Does anyone know about a law that is supposed to go into effect in 2011
that will require histotechs to be licensed? I also heard something
about a person being eligible to take the HT exam if they get a state
license - which can be obtained by simply paying a fee if they have 5
years of work
I am with you Hazel. We cut paraffin and today I am cutting GMA plastic with
them. They are great for us.
Pam Marcum
UPENN Vet School
New Bolton Center
- Original Message -
From: Hazel V Horn hor...@archildrens.org
To: histonet@lists.utsouthwestern.edu
Sent: Thursday,
Stacey,
Same for me too with Accuedge! But I also like Shandon MX35 Premier. Pack
of 50 blades may be over $100 (depends on your Fisher pricing). I use these
for both microtome and cyrostat sections. I've also used these for MMA
sections. Premier Plus should be good
Hello Histonetters,
I understand most of the professionals on this website are histology
professionals, however, I thought I would give it a try since I have not
found a cytology listserv yet. Does anyone know how many slides per day a
cytotechnologist would screen within a private lab setting
Hello Histonetters!
I was wondering if any one knows of any studies done on
Davidson's fixative and breast IHC. We have been using Davidson's for
our colons for about two years now (thanks to the Histonet) and I would
like to expand its use to our very fatty breast cases. Our head
I like both, but use the MX because they are cheaper, and they are, if not
quite as good as, certainly a much greater value than the AccuEdge blades (I
do think that on average I go through the MX a little faster, but they are
still cheaper!!). I alsoo like the MB22 from ThermoFisher for cutting
Barry:
Agree. Pick up ONE theme and ask for everybody to contribute. If
satisfactorily covered, pick another and so on.
René J.
--- On Thu, 4/2/09, Rittman, Barry R barry.r.ritt...@uth.tmc.edu wrote:
From: Rittman, Barry R barry.r.ritt...@uth.tmc.edu
Subject: RE: [Histonet] So... I Need Some
Laurie,
The bill that everyone has been quoting is AB 2156. It does not state
that histotechnicians will have to be licensed.
The ASCP has specific eligibility requirements for HT certification and at
this point they are not related to licensure in any state. Other states
that have
Our cytotechs screen 40 to 60 a day.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Alyssa
Peterson
Sent: Thursday, April 02, 2009 3:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet]
Christine:
Unfortunately our profession has no standards with regards of how we do things,
we tend to add twists even to recognized procesures, is in our histotechs
DNA. Each histolab processes tissues mostly according to its own
experience with previous results.
So I think that the best for
Is this a state bill, a federal bill or what. I have never heard of
this. And are these specified requirements also part of the bill?
Where can we read more about this bill?
Mike
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Hi Ana,
I work with insects and related organisms. What did you need to know?
-Damien Laudier
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Does anyone have a copy of the Tissue-Tek II manual that they would be
willing to share? (PDF preferred, hard-copy graciously accepted)
Thanks (in advance),
Bernie Ball
Duke University
A hat should be taken off when you greet a lady and left off for the
rest of your life. Nothing looks
Hi
Looking at the images this appears to be the prozone effect which is caused by
the primary antibody concentration being too high and has been well reported in
both immunohistochemistry, immunology and serology articles. Do a google serch
for prozone phenomenon and you will find an
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