Was there more to the question? You would not want to use any fixatives
containing metals, such as mercury, if you will be doing end-point
decalcification determination using x-rays.
Patsy Ruegg pru...@ihctech.net
Sent by: histonet-boun...@lists.utsouthwestern.edu
10/03/2011 11:27 AM
To
Was it decalcifying a tissue fixed in a formalin based fixative using a
hydrochloric acid containing decalcifying fluid? as even storage of HCl and
formalin in the same area is a bad plan.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
We are a research lab and don't do a big volume of special stains. We just
received a project for 35 slides that need Von Kossa Stain.
I acid-washed some small glass staining dishes-no rack required (in the past, we
only had a few requests).
Can I run slides in plastic slide holders (24-place)
Good Morning Histonetters!
I've been having a counterstain problem with my GMS and PAS-F. Both stains
work as they should until the green counterstain is applied. I cannot get
my tissue (control or patient) to uptake the counterstain. I have varied
time, temperature, even companies. I have
Hi Histonetters!!
What would make the perfect management position?
Is it the location?
Perhaps the type of lab?
How about the size of the staff/lab?
Maybe its the Hours? The Money and/or benefits?
Could it be that a particular situation is the next logical step in your
career?
More than
We have had this problem before. Make sure that the glacial acetic acid has
been added to the light green stock solution. Cured our problem!!!
Lynne Bell, HT (ASCP)
Histology Team Leader
Central Vermont Medical Center
130 Fisher Road
Berlin, VT 05641
802-371-4923
-Original Message-
Hi Histonetters!!
I hope everyone is having a great day. Here is a list of some of my
most exciting opportunities for histology techs. My clients offer
excellent pay, benefits and relocation assistance. These are full time
permanent positions.
Take a look and if you or someone you know might be
Thought of another question! What other vendors do people order special stain
kits? We have been using Poly Scientific, which is very good, but costly.
Thanks!
Peggy
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50
It has been my experience that you do not need to acid wash the glassware for
the silver nitrate step. The only critical parts are the time (5 minutes) in
silver nitrate (protected from light), the 3 dH2O rinses (also protected from
light), developing time (2 minutes) in sodium
Is anyone using a rabbit CD68 or Rabbit CD31 on pig tissue and would you be
willing to share your vendor and protocol?
Annette Featherstone
Supervisor SUNY @UB
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Bernice Frederick HTL (ASCP), Senior Research Tech at the Pathology
Core Facility of the Robert. H. Lurie Cancer Center at Northwestern
University in Chicago notes
Ann Preece states acid decal uses aqueous solutions of either formic, nitric,
or trichloroacetic acid. Other methods mentioned are
http://www.jewellerydropship.co.uk/mysite.php?html45
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I've used Rowley Biochemical for years: http://rowleybio.com/
Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]
I am looking to purchase a Processor and embedding center. I have received a
quote for VIP6 and a TEC5 but they seem to be a bit out of our price range.
Does anyone have another Processor/embedding center that they found to be
reliable?
Thanks
Mike
When you are buying VIP6 or TEC5 you are both buying pot notch technology +
quality.
For sure you will find others cheaper but of lesser quality.
You should think of this as if buying a new car:
Do you want to buy one cheaper but less reliable, or one that will give you
years of reliable
Oh I had a 1965 edition, but someone borrowed it and never brought it back.
I have since acquired the 1972 edition in good shape, but there was a 1959
edition. That would be a good one to have in the library.
Shirley
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
When running more than 5-10 slides at a time in a silver stain, a precipitate
will most likely form. It would be better to run them in small batches in
coplin jars to give you the necessary control needed.
Acid washing is fine, but just laboratory grade cleaner works as well.
I usually place
CLIA-88 requirements can be found here:
http://wwwn.cdc.gov/clia/regs/subpart_j.aspx. Local and state requirements may
vary.
Eric Gilchrist, Supervisor
UNC Oral and Maxillofacial Pathology Laboratory
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Although I appreciate individual opinions, do not overlook the Leica
line. Us old timers may recognize the names Sakura or Thermo (in
its many permutations) more readily, I'd put my money on Leica every
time (and I have - with processor, embedding center, microtome and
autostainer). They do not
Dear Histonetters,
Can you tell me what procedures you use to prevent DNA/RNA contamination of
tissue sections when handling multiple samples. Do you just wipe down blade
holder and blade (or use a new blade) between samples, or do you use more
stringent cleaning?
Thomas Crowell
Diagnostic
I still have my 1965 version..
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Ah, yes. That edition featured 105 illustrations, including 2
four-color plates (back in the day when that kind of stuff was worth
mentioning on the title page)
Have a great day - Bill
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Why is the von Kóssa method for calcified tissue still in use? It's
not very specific and requires handling silver. Why not alizarin red,
a simple dye that is more specific.
I've seen alizarin red S (C.I. 58005) used to demonstrate
microcalcifications in breast biopsy specimens. Obviously one can
Hello, For DNA, we use a new blande and we wipe down microtome, handles, front
of blade holder with 95% ethanol on a gauze sponge. For RNA, use a new blade
and wipe down all with RNAzap including the water bath and rinse with DEPC
water. Use clean water in bath, distilled will work or DEPC
We are just getting our second Excelsior and love them. It cuts tech time
changing bottles and paraffin as well as keeping us on track daily for changes
and rotations. We have to VIP, that are very old and one just died. That is
the one we are replacing with Thermo. The VIPs are great units
Anyone planning to attend the Fall Meeting of the South Carolina Society of
Histotechnology at Marina Inn in Myrtle Beach, SC November 4-5, 2011 NEEDS TO
MAKE YOUR RESERVATIONS!!! Number to call is 877-403-7676 and mention SCSHT for
special rate.
SC Histotechs and other members of SCSHT, this
Was Willey the dog you and sue had in VA? Seems he ate everything -
including an electrical cord -
-Original Message-
From: Podawiltz, Thomas [mailto:tpodawi...@lrgh.org]
Sent: Tuesday, October 04, 2011 1:27 PM
To: O'Donnell, Bill; Bob Richmond; histonet@lists.utsouthwestern.edu
I had a dachshund named Willey, he ate my copy, guess he like the pictures.
Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer.
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Need help with Congo Red for Amyloid problem. We have been using automated
staining with Dako's Artisan and recently tried manual procedure with fresh
reagents from Polyscientific kit with little to no staining on two commercially
prepared controls and two different patients who have been sent
Yes he was, and yes he did
Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220
From: O'Donnell, Bill [billodonn...@catholichealth.net]
Sent: Tuesday, October 04, 2011 2:29 PM
To: Podawiltz,
Kits ... Bah!
Just make it up. Save yourself a fortune and actually know what's in the
solutions. All the classical texts should have the procedures as well as
various websites like stainsfile.org IHCworld.com Otherwise just ask here
and I'm sure someone will have the information you need
Hi,
You will want to do more than just wiping it down. You should use
something specifically designed to clean such contamination like RNAase away
(Invitrogen if memory serves). You'll want to wipe down any surfaces that
come in contact with the block, sections or your hands when sectioning.
I am using rabbit CD31 (sc1506-R) from santacruz on rat tissue and it is
working fine.
Dr. Amita Dubey
PCSED,TRC
From: Featherstone, Annette a...@buffalo.edu
To: 'histonet@lists.utsouthwestern.edu'
histonet@lists.utsouthwestern.edu
Date: 04/10/11 09:06 PM
Subject:[Histonet]
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