RE: [Histonet] Specimen Tracking for courier

I would be interested in everyone responses. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manger X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bull,

RE: [Histonet] Automated Slide Stainer and Coverslipper: Foil or Glass?

Martin We currently have a Leica CV35030, which is a glass cover slipper. My 15 Pathologists prefer the glass covers slips for the optical quality. We are required to archive our slides for 20 years, which is another reason we chose glass cover slips. The Leica CV5030 if cleaned nightly and

[Histonet] HPV high low risk

Hi Histonetters I am looking to add to our test menu the High and low risk HPV. What is everyone using out there. Instrumentation? Vendor? Pros and cons to any system. I would appreciate any input. Thanks in advance. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manger X-Cell Laboratories

[Histonet] immuno stainers

Hello Histonetters Happy Tuesday. I hope everyone had a Merry Christmas. It is amazing how times flies when you have time off. Now it's back to the old laboratory grind. We are in the market for a new immuno stainer. I currently use a Dako Link, which I am very pleased with. I have demoed the

[Histonet] bleaching melanin

Hello Histonetters I need to bleach melanin out of some paraffin sections. The only bleaching solution I have is a 3% H2O2. I know you can bleach melanin in a 10% H2O2 solution for 1 to 2 days. How long do you think it will take to bleach the section in a 3% solution? Of course everyone wants it

[Histonet] question

Good Morning Histonetters One of our clients is interested in starting to compare biopsy tissue with a DNA swab. The test name is Known Error test and it is performed at a company in Utah. Does anyone out there know what this testing entails. I could use any information since I have no knowledge

RE: [Histonet] Humidity levels and IHC staining

NY in winter has low humidity, I still have no problems with my Dako stainer. Make sure the lid is closed. I had a tech who consistently left the lid open, there was some drying on the first row of slides. Closed the lid the problem stopped. Are you using Dako buffer? The surfactant in the buffer

[Histonet] egfr

Good Morning Histonetters I am looking to add EGFR to our antibody list. What clone is everyone using out there in Histoland? Any info would be greatly appreciated. Thanks in advance. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 Ext. 232 e-mail

RE: [Histonet] Cytokeratin AE1/AE3

Joanne What Dako platform are you using, link or 48? On the link I pretreat with PK, the IS series antibody is used with an incubation time of 10 minutes.. On the 48 I pretreat with High pH TRS, the IR antibody is used with an incubation time of 20 minutes. Both systems give me clean staining.(I'm

RE: [Histonet] Block Storage-another question

If the pod lab is in NJ and the reading lab is in NY, which guide lines do you follow. NYS requires us to save our blocks for 20 years. Due to storage issues, I would rather the pod lab store the blocks. Cindy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] know error system - prostate biopsies

We had one of our clients looking into this testing. The company does a hard sell. What they neglect to tell you is the insurance companies may not cover this testing leaving the patient with a huge bill sometimes $700 to $800. Known Error will tell you it is a Medicare approved test what they

RE: [Histonet] Batch Controls

What do you do if the IHC slides need to be sent to another facility. Currently we place a control on every slide we run. I know this is using a lot of tissue but I sometimes have 3 different cases going to 3 different facilities for patient treatment. Also we stain all the IHC stains for 5

RE: [Histonet] DeCloaking Chamber

Karen I would just purchase a timer to plug the Decloaking chamber into, I use one to heat my water bath for pretreatments prior to by techs coming in. I purchased the timers from Fisher, cat. #666224 for $28.82. Works like a charm. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell

[Histonet] vaginitis panel

Histonetters For those of you performing vaginitis panels, how are you billing these? Do you bill for each separate test or use the combine CPT code? We are having a discussion on what is our best method of billing. Thanks in advance for the info. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory

RE: [Histonet] vaginitis panel

I know. I was hoping to see what everyone else is doing. -Original Message- From: Kim Donadio [mailto:one_angel_sec...@yahoo.com] Sent: Friday, May 04, 2012 6:30 PM To: Cynthia Pyse Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] vaginitis panel It's Friday :) Noticed

RE: [Histonet] Xylene Substitute for Counterstain Clearing

Andrew You could use Clearium from Leica. Clearium can either be coverslipped from xylene or isopropyl alcohol. Drying time from isopropyl is longer then xylene. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cp...@x-celllab.com

RE: [Histonet] Eosin staining for small biopsies

We also use safranin on all our small biopsies and also our breast biopsies with no adverse effects to any of our IHC or ISH. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cp...@x-celllab.com -Original Message- From:

[Histonet] ENFD Testing

Happy Wednesday Histonetters I have had a request from some of our clients to do epidermal nerve fiber density testing. I have found information in how to obtain the specimen but have not found the protocol for processing, cutting, and staining the specimen. To anyone out there who is

RE: [Histonet] Bachelor's Degrees

Tom, I agree with you about Nate qualifications. Unfortunately in NYS if you bring in outside work your tech MUST be licensed in NY. The copy of the license need to be displayed in the lab. I haven't found any way to hire employees without a license. Cindy Pyse, CLT, HT (ASCP) Histology

RE: [Histonet] Re: Sakura iDent

Anne You might want to try going up the Sakura chain of command. I had trouble with a coverslipper, after contacting the regional manager it was amazing the service it received. Good luck. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com

RE: [Histonet] Tape/Film Coverslips vs. Glass

Cathy I prefer the glass coverslips. It seems to give more protection to the tissue. Recoverslipping is also easier than with the tape. Just my opinion. Hope this helps. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message-

RE: [Histonet] fontana

Fontana-Masson also stains for Argentaffin granuals. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of

RE: [Histonet] Helicobacter Pylori antibody

We purchase it from Biocare. Protocol: pretreatment Diva (Biocare) 20 minutes water bath 20 minutes cool down. Detection system Mach 4 (Biocare) 10 minutes H2O2, 10 minute primary antibody dilution 1:100, 5 minutes mouse probe, 12 minutes polymer, 10 minutes DAB(Dako), 5 minutes hematoxylin(Dako).

RE: [Histonet] p16

We purchase our p16 through MTM laboratories reference # 9517. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf

RE: [Histonet] Excelsior Vs. VIP 6

Matt We have both the VIP 6(new)and the Excelsior(older). The Excelsior is a little less user friendly but is a workhorse if maintained properly. There is less solution exposure since you do not have to fill the containers as you do in the VIP6. Both machines produce excellent tissue blocks. The

RE: [Histonet] Removing Yellow color from slides refixed in Bouin's solution

I just place the slides into 80% ETOH for 2 minutes. Cindy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of cscam...@uci.edu Sent: Monday, March 15, 2010 7:42 PM To: HistoNet Subject: [Histonet] Removing

[Histonet] Mc Coy fixative

Hi Histonetters I am having a senior moment. Can anyone tell me what McCoy fixative is made of and what it is used for? Thanks in advance for the help. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com

RE: [Histonet] coverslipper

Leica CV3050 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Thursday, March 25, 2010 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] coverslipper Hi Colleges, I

RE: [Histonet] histo techs doing cyto prep work

That is how I started many moons ago(29 years and counting). I spent 1 week in histology, the next week in cytology, this continued for 2 years. I currently supervise both the histology and the cytology lab sections. My histotechs do little cytology prep work but are willing if time allows to help

[Histonet] IHC on FNA

Happy Friday Histonetters What is everyone using for controls for FNA IHC stains. We routinely stain human FFPE sections and only have controls for tissue. Recently we had a few cases when the only sample was an FNA to perform IHC on. Any suggestions would be welcome . Regards Cindy Pyse,

RE: [Histonet] IHC on FNA

, April 09, 2010 12:40 PM To: Cynthia Pyse; Histonet Subject: RE: [Histonet] IHC on FNA This topic touches a nerve with me. We recently went back to receiving requests on FNAs after years of not doing any. When we were routinely staining them with IHC, we would use a frozen tissue section

[Histonet] Thanks

Thanks to all for providing me with the information I requested. You have made my decision making so much easier. Thanks again Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet

[Histonet] CD68

Happy Friday Everyone What clone is everyone using for the CD68 antibody for FFPE human tissue? Thanks for the info in advance. Everyone have a great weekend. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com

RE: [Histonet] Gastric biopsies

Christi We run a diff stain on all gastric bx suspected of H. pylori as routine, it saves us time. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Is anyone else having an issue with their paraplast?

I'm relieved that someone else is having the same problem. I was beginning to think it was my processor. I have already decided to switch paraffin. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From:

RE: [Histonet] water bath information

I am also looking for the same type of waterbaths and would appreciate any information. Thanks Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] BILLING QUESTION

We have a contract with the hospitals, this comes from our billing manger. We bill the hospitals, then they bill the patient. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From:

RE: [Histonet] inking dyes

I use tattoo dyes. They work great and are much cheaper than tissue marking dyes. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Biocare P63 with Dako Flex detection

Cathy Have you tried adding an EnVision FLEX mouse linker to your protocol. Sometimes that will work. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Water collecting at bottom of sections

I second Linda's technique, the only other option I use is to bring the slide up at a negative angle so the slide comes out of the water bath first then the section. I find this works well for us. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com

[Histonet] Sakura VIP6

Help! I am having a problem with me VIP6 tissue processor. About every 4 to 6 weeks the processor has a error code that position 2 which is 80% has not pumped in fully in the allotted time. Sakura answers to this problem is either I need to use their formalin or the alcohol in the second station

[Histonet] Sakura VIP6 update

Thanks everyone for your responses. Sakura has scheduled an upgrade of my rotary and gate valve. I hope this is the answer I need. Cindy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] Tissue Processors

Rhonda I have both a Thermo-Fisher Excelsior and a Sakura VIP6. Both work well but I prefer the Excelsior. There is less hands on time for changing reagents, flushing and cleaning. I did have problems at first with the VIP. Since Sakura replaced the gate valve all has been great. If I need to

RE: [Histonet] Waste drum labeling

I would also be interested in the answers to this. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com -Original Message- From:

[Histonet] chemical disposal

Quick question for Histoland. I am having a debate about DAB disposal. Our general manager ( non lab background) insists that the liquid DAB can go into a biological hazardous waste. I disagree, it is a chemical and needs to be disposed in the chemical hazardous waste. What is everyone else doing

[Histonet] thanks

I want to thank everyone who responded to my chemical disposal question. I now have adequate ammunition to combat my general manager's lack of knowledge. He seems to think I don't know what I am talking about. Thanks again for the backup it is appreciated. Have a great weekend. Cindy Cindy

RE: [Histonet] TISSUE PROCESSOR FOLLOW-UP QUESTION

Yes you are. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] moleculer testing

Happy Monday Histonetter Who out there in Histoland is doing the testing for BRAF, KRAS, ALK, and HER2neu and what testing methods are you using. I prefer not to use FISH. Would rather have a permanent record of the slides. Any information is welcome. Have a great week. Cindy Cindy Pyse,

[Histonet] her 2 neu, CISH vs FISH

Good afternoon histonetters We are currently investigating bringing Her 2 Neu CISH staining into our lab. FISH staining has too many logistics nightmares for me to consider, not to mention the cost of purchasing digital scanning equipment. Our pathologist are off site in 5 different hospitals.

[Histonet] problem with 88305 reimbursement

Hi Histonetters We are currently having a problem with our Medicare reimbursement on the tech component of the 88305. If the patient has been seen at a hospital, either in-patient or out- patient, we are told by Medicare that we have to contract with that hospital to bill for the 88305. The

RE: [Histonet] problem with 88305 reimbursement

) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com -Original Message- From: Brendal Finlay [mailto:brendal.fin...@medicalcenterclinic.com] Sent: Tuesday, January 29, 2013 2:32 PM To: Cynthia Pyse Cc: histonet

[Histonet] p16

Happy Wednesday Everyone Has anyone been having consistency problems with their p16 staining since MTM has been taken over by Ventana? One day the procedure works another day it doesn't. Same procedure, no variation in the temperature on pretreatment, run on the Dako 48, with no errors.

FW: [Histonet] p16

-celllab.com -Original Message- From: Ross Benik [mailto:r...@premierlab.com] Sent: Wednesday, February 06, 2013 1:26 PM To: Cynthia Pyse Subject: RE: [Histonet] p16 Do you monitor the temperature and humidity inside the Link48? How often do you prepare your retrieval solution/are you using

[Histonet] FW: DAB

From: Cynthia Pyse [mailto:cp...@x-celllab.com] Sent: Tuesday, September 28, 2010 3:48 PM To: 'histonet-requ...@lists.utsouthwestern.edu' Subject: DAB Hello Histonetters I need some expert advice concerning the disposal of DAB. Our lab is in NYS and there has been some discussion

[Histonet] gloves

Happy Monday Histonetters I am looking for a reasonably priced, non-latex glove that will hold up to xylene. The purple knight from Kimberly-Clark works great but is cost prohibited. My general manager want me to find a cheaper alternative. I have had several samples but none will hold up to any

[Histonet] Viper Affrim

Hello Histonetters We have recently added the BD Viper and Affrim testing to our lab. Training is underway. I would like input from the experts out there on how much time is actually spent on setting up both the Viper and Affrim testing. I know what the manufacturer says I just want the opinion

[Histonet] IHC Equipment

Hi Histonetters I currently use a Dako stainer for my IHC staining. It is a work horse with very little problems. It is a older model that we may need to replace in the near future. What is everyone using out in histoland. I would be perfectly willing to purchase another Dako but I want to

RE: [Histonet] procedural safeguards when accessioning identical sources

Carol We mark all our prostates with dye for ease when embedding. For prostates that need to be accessioned in order we alternate the color of the dyes, hematoxylin, safranin, eosin. When grossing, the color of the dye used is in the dictation. Hope this helps. Cindy Cindy Pyse, CLT, HT (ASCP)

[Histonet] Thanks

Happy Friday Histonetters I want to thank everyone for the information on IHC strainers. This is just what I needed. People who actually use the machines on a daily basis giving their opinions. What a great forum we have. Hope everyone has a great weekend. Currently we are receiving 6-10 inches

RE: [Histonet] How many tissues an histotech is suppose to cut per hour?

Practice, practice, practice! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Monday, February 28, 2011 5:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an

RE: [Histonet] how to bleach melanoma cases without bad effect on IHC

Sabeti I use the AEC chromogen (Dako catalog #K346989) for my melanoma cases. I use a aqueous mounting media, placed on the tissue only, dry it completely on a hot plate. When dry I coverslip it with permanent mounting media(don't place it in xylene), dry coverslip. Works great, and the color

RE: [Histonet] HercepTest

Cindy I use a Fisher Isotemp 205 waterbath. The end result is beautiful. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Immuno's

Do you use a hydrophobic pen to circle the tissue. This concentrates the solution where you need it. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 etx.232 e-mail cp...@x-celllab.com -Original Message- From:

[Histonet] cyto prep

Good Morning Histonetters For those of you who have pap smears in their facility's, who do you have preparing the pap smears. Lab assistants, lab tech, or lab technologists? I am having a discussion with my general manager on who can prep pap smears. I decided to take a quick poll to see what

[Histonet] Leica Bond III

Hi Histonetters I am testing the Leica Bond III immunostainer. How is everyone handling processing the Hercept test from Dako on the Bond III? I cannot use either the approved pretreatment or visualization system, not to mention the approved buffer. I currently run my immunos on the Dako so it

[Histonet] decal

Hello Histonetters What is everyone using to decal specimens. I haven't had to use it in a few years, before that I just purchased formic acid and made a 10% solution. Just wanted to see if the criteria has changes over the years. Thanks in advance for the responses. Cindy Pyse, CLT, HT

[Histonet] validation sheet

Happy Friday Histonetters Does anyone have a validation log sheet they have used for the validation of a recycler. Someone spilled xylene on our copy then proceeded to discard it without coping the sheet first. I would appreciate it if anyone would share the log sheet they have used, it would

RE: [Histonet] Retirement

As long as we don't have to go back to steel knives, old AO micrtomes and the autotechnicon I'm in. I am also 15 to 18 years out. What is the world of histology going to do without us. Who will know how to make a solution of mucicarmine (not that I do anymore, but I could) or eosin? The tech I

[Histonet] water

Hi Histonetters I have 2 tech off on vacation so I thought I would take the easy way this time. Does anyone know the difference between distilled water system and the reverse osmosis water system? Could if effect the IHC staining by using one or the other? Usually I would research it myself but

RE: [Histonet] alcohol as a fixative

Most clinician I know have other staff in the procedure room. Why not have someone other than the clinician place the specimen in the formalin. This way you get the best possible fixation for the patient. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories e-mail

[Histonet] cam 5.2

Hello Histonetters What company is everyone buying their Cam 5.2 antibody from? Thanks for the information. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list

[Histonet] Cam 5.2

Thanks to everyone for the Cam5.2 info. A great weekend to all. Cindy Cindy Pyse, CLT, HT (ASCP) Laobratory/Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] Old Green Staining Racks

Market Lab cat#ML1212. I would check the cat# before ordering, the picture in the catalog show the right rack but the description is different. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From:

[Histonet] unstained slides

Hello Histonetters, Recently we have seen an increase number of unstained slides requested from outside facilities. We do not release our blocks, so unstained slides are the only option for any facility to obtain our tissue. Currently we do not charge for these slides, but I am rethinking that