Re: [Histonet] Tissue Processor Down Question

2024-03-14 Thread Diana Martinez-Longoria via Histonet 
Okay, thank you for your advice.

Thank you,
Diana Martinez-Longoria
El Centro Regional Medical Center
Lead Histotechnician (ASCP)cm
Laboratory - Pathology Department
1415 Ross Ave | El Centro, CA  92243
760.339.7267: Fax: 760-3394570
 diana.martinez-longo...@ecrmc.org



-Original Message-
From: Gudrun Lang [mailto:gu.l...@gmx.at] 
Sent: Thursday, March 14, 2024 7:28 AM
To: Diana Martinez-Longoria
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] Tissue Processor Down Question

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Depending on the time the cassettes were in clearing medium, I would transfer 
them into molten paraffin at least for double of the time in the processor. And 
then embed etc.
Try a few blocks to see, if infiltration was sufficient. If cutting is 
hampered, prolong the time in paraffin.
Gudrun

-Ursprüngliche Nachricht-
Von: Diana Martinez-Longoria via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Gesendet: Donnerstag, 14. März 2024 13:59
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Tissue Processor Down Question
Wichtigkeit: Hoch

Good morning,

Our VIP5 tissue processor went down and our tissue cassettes were in clearing 
solution. What is the best media to put them while we wait for the tissue 
processor to be repaired, please let me know?
Thank you,
Diana Martinez-Longoria
El Centro Regional Medical Center
Lead Histotechnician (ASCP)cm
Laboratory - Pathology Department
1415 Ross Ave | El Centro, CA  92243
760.339.7267: Fax: 760-3394570
 diana.martinez-longo...@ecrmc.org




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Re: [Histonet] Tissue Processor Down Question

2024-03-14 Thread Gudrun Lang via Histonet 
Depending on the time the cassettes were in clearing medium, I would
transfer them into molten paraffin at least for double of the time in the
processor. And then embed etc.
Try a few blocks to see, if infiltration was sufficient. If cutting is
hampered, prolong the time in paraffin.
Gudrun

-Ursprüngliche Nachricht-
Von: Diana Martinez-Longoria via Histonet
[mailto:histonet@lists.utsouthwestern.edu] 
Gesendet: Donnerstag, 14. März 2024 13:59
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Tissue Processor Down Question
Wichtigkeit: Hoch

Good morning,

Our VIP5 tissue processor went down and our tissue cassettes were in
clearing solution. What is the best media to put them while we wait for the
tissue processor to be repaired, please let me know?
Thank you,
Diana Martinez-Longoria
El Centro Regional Medical Center
Lead Histotechnician (ASCP)cm
Laboratory - Pathology Department
1415 Ross Ave | El Centro, CA  92243
760.339.7267: Fax: 760-3394570
 diana.martinez-longo...@ecrmc.org




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Re: [Histonet] Tissue Processor Schedule Validations

2023-03-23 Thread Cooper, Brian via Histonet 
WOW!  I came back from lunch and I have seven responses already!  Everyone said 
the same thing, which was my game plan, take punches from larger samples!  
Happy Friday Eve everyone!   

Thanks so much Histonet!  

Brian

-Original Message-
From: Cooper, Brian via Histonet  
Sent: Thursday, March 23, 2023 11:54 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Schedule Validations (EXTERNAL EMAIL)

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Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of 
Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] Tissue Processor Schedule Validations

2023-03-23 Thread Paula Sicurello via Histonet 
The one thing that bothers me about the preloaded programs is the paraffin 
temperature is set at 65 degrees C.  That's close to 10 degrees higher than the 
melting point of our paraffin.
Does anyone know why the temp. is so high?  To me, a temp that high will cause 
problems with IHC and small biopsies.  
Has anyone changed it?  Or do folks go along with it just because it's already 
there? 
I inherited that temp, to change it now would be pain since it is the one we 
use the most.  If I validated our current Peloris 3, I would have set the wax 
temperatures at 60 degrees.
Sincerely,
Paula Sicurello 

On Thursday, March 23, 2023 at 11:54:44 AM PDT, Cooper, Brian via Histonet 
 wrote:  
 
 Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] Tissue Processor Schedule Validations

2023-03-23 Thread Thomas Podawiltz via Histonet 
I ordered small punch biopsy kits and made my own small biopsies from remnant 
tissue. Renal biopsy kits worked for making our own needle biopsies. Leica 
already has the protocols that work so part is easy. 


Sent from Yahoo Mail for iPad


On Thursday, March 23, 2023, 3:05 PM, Normington, Lacy via Histonet 
 wrote:

We have purchased several disposable core biopsy instruments of varying gauges 
and take samples of large resection specimens. We also use the same grossing 
tools to take small samples of GI, endo, ecc, ect.

Lacy
 

-Original Message-
From: Cooper, Brian via Histonet  
Sent: Thursday, March 23, 2023 1:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Schedule Validations

WARNING: This email appears to have originated outside of the UW Health email 
system.
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Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of 
Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] Tissue Processor Schedule Validations

2023-03-23 Thread Normington, Lacy via Histonet 
We have purchased several disposable core biopsy instruments of varying gauges 
and take samples of large resection specimens. We also use the same grossing 
tools to take small samples of GI, endo, ecc, ect.

Lacy
 

-Original Message-
From: Cooper, Brian via Histonet  
Sent: Thursday, March 23, 2023 1:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Schedule Validations

WARNING: This email appears to have originated outside of the UW Health email 
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know 
the content is safe.

Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of 
Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] Tissue Processor Schedule Validations

2023-03-23 Thread Whitaker, Bonnie via Histonet 
Put in two cassettes of resection tissue that are cut to mimic biopsy 
sizes/tissue (colon resection for colon bx, liver resection for liver bx, etc.

Bonnie Whitaker
Ohio State University
AP Operations Director

614-293-8418

-Original Message-
From: Cooper, Brian via Histonet  
Sent: Thursday, March 23, 2023 2:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Schedule Validations

Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of 
Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] Tissue processor advice

2020-09-17 Thread Bacon, Charles via Histonet 
Hi Colleen,

We have all VIPs here at BMC but I have used the Leica in the past at a 
previous position. We currently have 3 - E300s, 3 - VIP 5s, and 2 - VIP 6s. The 
VIPs are great and really only need preventive stuff like O rings and 
lubrication. A service company for us Avantik takes great care of all of that. 
You can get refurbished VIPs at great prices and they will last!

Chuck Bacon, HTL(ASCP)CM 
Supervisor Histology
Baystate Medical Center
361 Whitney Ave., Holyoke, MA 01040
Telephone: 413-322-4786  Fax: 413-322-4790
charles.ba...@baystatehealth.org

-Original Message-
From: Colleen Forster  
Sent: Wednesday, September 16, 2020 1:17 PM
To: histonet-request 
Subject: [Histonet] Tissue processor advice

HEllo Histoneters,

I am looking at replacing my VIP2000, porr girl finally quit on me.

The two processors I am looking at:

Leica ASP300S
Sakura VIP5

Any of you out there who have used either of these or both, can you give me
pros , cons, yes, noI am just looking for experiences those who have
used them can share.

Thank you in advance.

Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
612-626-1930



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Re: [Histonet] Tissue processor validation

2020-09-02 Thread Simmons, Christopher via Histonet 
Get your gross lab to provide you with samples representative of what you 
normally process and the same for tissue you use for internally procured 
special stain controls (kidney, lung, etc)
Process as usual and cut and perform specials
If they work: done.

Get Outlook for iOS

From: Martha Ward-Pathology via Histonet 
Sent: Wednesday, September 2, 2020 3:51:42 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Tissue processor validation

I am posting this question for my colleague in the Histology lab.   She is 
working on a plan for a new tissue processor validation, including special 
stains, and would like to get input from anyone that has already gone through 
it.This is the first time anyone here has ever been involved in validating 
a new processor.   Current ones are 25+ years.


Any help or advice would be appreciated.   Thanks in advance!

Martha Ward, MT (ASCP) QIHC
Wake Forest Baptist Health
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Re: [Histonet] Tissue processor errors, failures and what to do

2020-07-04 Thread E. Wayne Johnson via Histonet 

Automation is a wonderful thing but it is only a replacement for what people 
used to do by hand.
We have incubators that can be set to 60C and we have a Rube Goldberg-ized 
microwave oven with
a thermal controller and relays (and the not-to-be-forgotten flyback diode) and
a K-type thermal probe covered with aluminum foil (dont try this at home, kids)
so we are ready for any incident or mishap with our cantankerous VIP5.

There is no such thing as a convenient time for it to quit but lots of times 
the trouble
is that it was time to change the solutions and nobody did it even though
anybody could have done it.

If there is nothing we can do to get the VIP5 going again pronto
we finish the processing by hand.
there are repair manuals for some of those machines available online
or you can call the repairman.  If you are out in Bufooee somewhere the repair
manual and the secret repair modes on the machine could be the difference 
between joy
and the slough of despond.

E. Wayne Johnson DVM
Enable AgTech
Beijing

Patpxs via Histonet wrote:

Hi Garrey,

The answer is “it depends”.   What you do when a processor fails depends on the 
failure point.  If the tissue is still in dehydrant it gets treated differently 
than if it fails in the intermediate solvent.

Paula

Sent from my iPhone


On Jul 4, 2020, at 10:08 AM, Garrey Faller via Histonet 
 wrote:

Happy 4th to all.
Does anyone have a procedure on what to do when a tissue processor fails or 
alarms.  I want to learn more about the science behind tissue processing so I 
know what to do when the machine fails. This happened to a friend recently and 
I want to prevent my tissues/biopsies from being ruined.
Thanks.
Garrey

Sent from my iPhone
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Re: [Histonet] Tissue processor errors, failures and what to do

2020-07-04 Thread Patpxs via Histonet 
Hi Garrey,

The answer is “it depends”.   What you do when a processor fails depends on the 
failure point.  If the tissue is still in dehydrant it gets treated differently 
than if it fails in the intermediate solvent.  

Paula

Sent from my iPhone

> On Jul 4, 2020, at 10:08 AM, Garrey Faller via Histonet 
>  wrote:
> 
> Happy 4th to all.
> Does anyone have a procedure on what to do when a tissue processor fails or 
> alarms.  I want to learn more about the science behind tissue processing so I 
> know what to do when the machine fails. This happened to a friend recently 
> and I want to prevent my tissues/biopsies from being ruined.
> Thanks.
> Garrey
> 
> Sent from my iPhone
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Re: [Histonet] Tissue Processor

2018-02-05 Thread Terri Braud via Histonet 
After 40 years of using primarily Miles/Tissue Tek, we recently purchased 2 
Leica ASP 6025.  With the exception of a small glitch with faulty sensors, and 
a learning curve to get used to them, I would highly recommend them.  We had 
replaced an ancient K series(with the magnet) and a VIP 5.
The quality and speed of the processing from the Leica's is incredible.  We had 
great tech support in setting up and validating processing protocols.  We ended 
up using several of their pre-set protocols which worked great, especially for 
the processing of large tissues.  I was expecting better processing than our 
ancient Tissue Tek, but the idea that it outperformed the VIP 5 surprised me.  
Reagents are a snap to exchange and the software/screen is very user friendly.
I hope this helps, Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
Today's Topics:

   1. tissue processor (Lisa Brenner)
--
Message: 1
From: "Lisa Brenner" 
Subject: [Histonet] tissue processor
Hello,
   We are in the market for a new vacuum infiltrating tissue processor. Our old 
VIP has been a work horse but needs replacing. What is everyone using? What 
works well? What's new?
Lisa Brenner
**


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Re: [Histonet] tissue processor

2018-02-02 Thread Cooper, Brian via Histonet 
We absolutely love our Leica Peloris II.

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Lisa Brenner via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, February 02, 2018 10:36 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] tissue processor (EXTERNAL EMAIL)

Hello,

   We are in the market for a new vacuum infiltrating tissue processor. Our old 
VIP has been a work horse but needs replacing. What is everyone using? What 
works well? What's new?

Lisa Brenner


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RE: [Histonet] Tissue Processor Validation Procedure

2015-01-31 Thread Jamal 
Hi Allison
I hope the attached can help you



Best Regards,


Jamal M. Al Rowaihi Anatomic Pathology Supervisor   | Al Borg
Medical Laboratories |  Mobile +966 503629832|
j.rowa...@alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA|
Phone: +966 12 670 0099   | Fax: +966 12 676 4984 |
www.alborglaboratories.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott,
Allison D
Sent: Thursday, January 29, 2015 10:44 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Validation Procedure

Hello to all in Histoland.  Does anyone have a tissue processor validation
procedure that they would be willing to share?  Any help in this would be
greatly appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
713-566-5287(Lab)
713-566-2148(Office)

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RE: [Histonet] Tissue Processor Validation Procedure

2014-11-08 Thread Jamal 
Dear colleague
I hope the attached help you



Best Regards,


Jamal M. Al Rowaihi Anatomic Pathology Supervisor   | Al Borg
Medical Laboratories |  Mobile +966 503629832|
j.rowa...@alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA|
Phone: +966 12 670 0099   | Fax: +966 12 676 4984 |
www.alborglaboratories.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adesupo,
Adesuyi (Banjo)
Sent: Thursday, November 06, 2014 11:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Validation Procedure

Hi,
   I am wondering if you guys in histoland will like to share your Tissue
Processor Validation Procedure with me.

Thanks,
Adesuyi
==
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RE: [Histonet] Tissue Processor Validation:

2014-09-06 Thread Jamal 
Hi Jb
The attached is my validation form you can refer to it.


Best Regards,


Jamal M. Al Rowaihi Anatomic Pathology Supervisor   | Al Borg
Medical Laboratories |  Mobile +966 503629832|
j.rowa...@alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA|
Phone: +966 12 670 0099   | Fax: +966 12 676 4984 |
www.alborglaboratories.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb
Sent: Friday, September 05, 2014 9:11 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Validation:

Does anyone have a tissue processor validation form that they are willing to
share?

Thank you. 

Sent from my iPhone
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Re: [Histonet] Tissue Processor Validation:

2014-09-06 Thread Mike Andrews 
It appears that the list software strips attachments. You may want to put it on 
a website and post the URL.

Mike Andrews, W5EGO
WWME Oklahoma area executive team

 On Sep 6, 2014, at 4:34 AM, Jamal j.rowa...@alborglaboratories.com wrote:
 
 Hi Jb
 The attached is my validation form you can refer to it.
 
 
 Best Regards,
 
 
 Jamal M. Al RowaihiAnatomic Pathology Supervisor   | Al Borg
 Medical Laboratories |  Mobile +966 503629832|
 j.rowa...@alborglaboratories.com 
 Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA|
 Phone: +966 12 670 0099  | Fax: +966 12 676 4984 |
 www.alborglaboratories.com
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb
 Sent: Friday, September 05, 2014 9:11 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Tissue Processor Validation:
 
 Does anyone have a tissue processor validation form that they are willing to
 share?
 
 Thank you. 
 
 Sent from my iPhone
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RE: [Histonet] Tissue Processor Validation

2013-09-01 Thread tahseen 

Dear All
Would any one of you out there,want to share template of Tissue 
Processor validation forms?

Thanks
Muhammad Tahseen
Histolog Supervisor
SKMCHRC Lahore
Pakistan

On 2013-08-28 19:10, Horn, Hazel V wrote:

We did the same as Beth.

Hazel Horn
ySupervisor of Histolog/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth
Brinegar
Sent: Wednesday, August 28, 2013 8:40 AM
To: Rathborne, Toni
Cc: histonet@lists.utsouthwestern.edu; dianar...@aol.com
Subject: Re: [Histonet] Tissue Processor Validation

Yes, we do a few validation runs. Nothing too extreme, however we
gross some extra patient tissue and do a small run, then increase the
amount of blocks processed until we are ready to run cases for 
patient

diagnosis. It really only takes a week or two. After those blocks are
cut, then we compare them to the previous processor results and make
changes if neccessary.

Beth Brinegar HTL(ASCP)
Anatomic Pathology Supervisor
Mercy Medical Center
Cedar Rapids, IA 52403


On Wed, Aug 28, 2013 at 8:27 AM, Rathborne, Toni 
trathbo...@somerset-healthcare.com wrote:


How do you validate IHC  FISH with a new processor?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [mailto:
histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle 
weaver

Sent: Wednesday, August 28, 2013 9:23 AM
To: dianar...@aol.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Tissue Processor Validation

Yes definitely, 5 programs, 25-50 sister, serial sections on tissue
that represents patient samples.




Joelle Weaver MAOM, HTL (ASCP) QIHC

 From: dianar...@aol.com
 Date: Tue, 27 Aug 2013 22:07:19 -0400
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Tissue Processor Validation

 I am interested in knowing how many of you out there Validate your
 Tissue Processors before you put them into use and how extreme you 
go.


 Diana Ripley
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Re: [Histonet] Tissue Processor Validation

2013-08-28 Thread Beth Brinegar 
Yes, we do a few validation runs. Nothing too extreme, however we gross
some extra patient tissue and do a small run, then increase the amount of
blocks processed until we are ready to run cases for patient diagnosis. It
really only takes a week or two. After those blocks are cut, then we
compare them to the previous processor results and make changes if
neccessary.

Beth Brinegar HTL(ASCP)
Anatomic Pathology Supervisor
Mercy Medical Center
Cedar Rapids, IA 52403


On Wed, Aug 28, 2013 at 8:27 AM, Rathborne, Toni 
trathbo...@somerset-healthcare.com wrote:

 How do you validate IHC  FISH with a new processor?

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
 Sent: Wednesday, August 28, 2013 9:23 AM
 To: dianar...@aol.com; histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] Tissue Processor Validation

 Yes definitely, 5 programs, 25-50 sister, serial sections on tissue that
 represents patient samples.




 Joelle Weaver MAOM, HTL (ASCP) QIHC

  From: dianar...@aol.com
  Date: Tue, 27 Aug 2013 22:07:19 -0400
  To: histonet@lists.utsouthwestern.edu
  Subject: [Histonet] Tissue Processor Validation
 
  I am interested in knowing how many of you out there Validate your
  Tissue Processors before you put them into use and how extreme you go.
 
  Diana Ripley
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RE: [Histonet] Tissue Processor Validation

2013-08-28 Thread joelle weaver 
 On Wed, Aug 28, 2013 at 8:27 AM, Rathborne, Toni  
trathbo...@somerset-healthcare.com wrote: 
  How do you validate IHC  FISH with a new processor?
 
You are going to have to revalidate IHC and FISH with new processing. How much 
might depend on if you are significantly changing instrumentation/technology, 
or just getting a new instrument of the same type. I usually don't do a full, 
as new assay validation, but the amount needed just to show that the processing 
change has not affected the test results. If there are issues that appear with 
that, you might have to do a complete revalidation. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
 From: hor...@archildrens.org
 To: bbrinegar...@gmail.com; trathbo...@somerset-healthcare.com
 Date: Wed, 28 Aug 2013 09:10:39 -0500
 Subject: RE: [Histonet] Tissue Processor Validation
 CC: histonet@lists.utsouthwestern.edu; dianar...@aol.com
 
 We did the same as Beth.
 
 Hazel Horn
 Supervisor of Histology/Autopsy/Transcription
 Anatomic Pathology
 Arkansas Children's Hospital
 1 Children's Way | Slot 820| Little Rock, AR 72202
 501.364.4240 direct | 501.364.1241 fax
 hor...@archildrens.org
 archildrens.org
 
 
 
 
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar
 Sent: Wednesday, August 28, 2013 8:40 AM
 To: Rathborne, Toni
 Cc: histonet@lists.utsouthwestern.edu; dianar...@aol.com
 Subject: Re: [Histonet] Tissue Processor Validation
 
 Yes, we do a few validation runs. Nothing too extreme, however we gross some 
 extra patient tissue and do a small run, then increase the amount of blocks 
 processed until we are ready to run cases for patient diagnosis. It really 
 only takes a week or two. After those blocks are cut, then we compare them to 
 the previous processor results and make changes if neccessary.
 
 Beth Brinegar HTL(ASCP)
 Anatomic Pathology Supervisor
 Mercy Medical Center
 Cedar Rapids, IA 52403
 
 
 On Wed, Aug 28, 2013 at 8:27 AM, Rathborne, Toni  
 trathbo...@somerset-healthcare.com wrote:
 
  How do you validate IHC  FISH with a new processor?
 
  -Original Message-
  From: histonet-boun...@lists.utsouthwestern.edu [mailto:
  histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
  Sent: Wednesday, August 28, 2013 9:23 AM
  To: dianar...@aol.com; histonet@lists.utsouthwestern.edu
  Subject: RE: [Histonet] Tissue Processor Validation
 
  Yes definitely, 5 programs, 25-50 sister, serial sections on tissue 
  that represents patient samples.
 
 
 
 
  Joelle Weaver MAOM, HTL (ASCP) QIHC
 
   From: dianar...@aol.com
   Date: Tue, 27 Aug 2013 22:07:19 -0400
   To: histonet@lists.utsouthwestern.edu
   Subject: [Histonet] Tissue Processor Validation
  
   I am interested in knowing how many of you out there Validate your 
   Tissue Processors before you put them into use and how extreme you go.
  
   Diana Ripley
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 message to the intended recipient, you are hereby notified that any 
 dissemination, distribution or copying of this communication is strictly 
 prohibited. If you have received this communication in error, please notify 
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RE: [Histonet] tissue processor remote alarm systems

2013-02-14 Thread Dessoye, Michael J 
I have a Sensaphone unit.  It can handle four different instruments.
When it calls it will give you the phone number it's calling from, the
sensor (instrument) number, and the 'alert condition' (there are
several...for example 1 is power failure, etc.)  Any other questions
about it I'm happy to help.

Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General
Hospital | An Affiliate of Commonwealth Health |
mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre,
PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 
 

-Original Message-
From: Morken, Timothy [mailto:timothy.mor...@ucsfmedctr.org] 
Sent: Thursday, February 14, 2013 11:01 AM
To: Histonet
Subject: [Histonet] tissue processor remote alarm systems

I'd like to hear from labs that use a remote messaging system for their
VIP5 tissue processors that are left unattended overnight. One I saw on
old histonet posts is Sensaphone.

I am wondering what kind of information you can get from the system.
Does it just say there is a problem but no details?

Can you connect several processors to one sensing unit? Can it tell the
difference between the units (ie, processor 1 vs processor 4?).

Thanks for any info!

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department
of Pathology UC San Francisco Medical Center


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RE: [Histonet] TISSUE PROCESSOR FOLLOW-UP QUESTION

2012-12-07 Thread Cynthia Pyse 
Yes you are.
Cindy

Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell Laboratories
20 Northpointe Parkway Suite 100
Amherst, NY 14228
716-250-9235 etx. 232
e-mail cp...@x-celllab.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock
Sent: Friday, December 07, 2012 12:44 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] TISSUE PROCESSOR FOLLOW-UP QUESTION

Hi All:

First, thank you for all your feedback on the processors.

I site-visited a VIP6 at a local hospital  From my understanding the VIP6
can rotate absolute alcohol and xylene (using the bulk reservoirs), as well
as the paraffin stations, but it cannot rotate other concentrations of
alcohol based on hydrometer readings.
Am I correct in this appraisal?

Tim Wheelock
Neuropathology Laboratory
Harvard Brain Bank
McLean Hospital
Belmont, MA

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Re: [Histonet] Tissue Processor

2012-08-08 Thread Rene J Buesa 
Sakura
René J.



From: Heckford, Karen - SMMC-SF karen.heckf...@dignityhealth.org
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Wednesday, August 8, 2012 8:11 AM
Subject: [Histonet] Tissue Processor

I am going to need to purchase a new tissue processor mine keeps breaking 
down.  What tissue processor would you buy and why?  I would greatly appreciate 
the help.

Cheers,

Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org
                                                                                
  Caution:  This email message, including all content and attachments, is 
CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.  The 
information contained in this email message is intended only for the use of the 
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Re: [Histonet] Tissue Processor

2012-08-08 Thread Pam Marcum 


We have swithched from the VIP Sakura, older models, to the Thermo Excelsior.  
We are currently buying our 3rd and 4th units for the Histology Lab.  They have 
features we really liked and cut my our exposure to formalin and xylene by 
almost 90% due to the way the system changes solutions.  Due to the very gently 
agitation used during processing we were also able to cut some time off our 
programs for overnight and short cycle biopsy runs.  



We stil use a Leica ASP 300 for bone marrows only as it is what need due to 
reagent requirements for xylene sub on the last two stations.  



If you have any questions let me know. 


Pam Marcum 

UAMS 



- Original Message -
From: Karen - SMMC-SF Heckford karen.heckf...@dignityhealth.org 
To: histonet@lists.utsouthwestern.edu 
Sent: Wednesday, August 8, 2012 7:11:13 AM 
Subject: [Histonet] Tissue Processor 

I am going to need to purchase a new tissue processor mine keeps breaking down. 
 What tissue processor would you buy and why?  I would greatly appreciate the 
help. 

Cheers, 

Karen Heckford HT ASCP CE 
Lead Histology Technician 
St. Mary's Medical Center 
450 Stanyan St. 
San Francisco, Ca. 94117 
415-668-1000 ext. 6167 
karen.heckf...@dignityhealth.org 
                                                                                
  Caution:  This email message, including all content and attachments, is 
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information contained in this email message is intended only for the use of the 
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you have received this document in error.  Any further review, dissemination, 
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Re: [Histonet] Tissue processor

2011-10-04 Thread Rene J Buesa 
When you are buying VIP6 or TEC5 you are both buying pot notch technology + 
quality.
For sure you will find others cheaper but of lesser quality.
You should think of this as if buying a new car:
Do you want to buy one cheaper but less reliable, or one that will give you 
years of reliable transportation?
It is the same thing with histology instruments!
René J.

--- On Tue, 10/4/11, Mike Tighe mti...@trudeauinstitute.org wrote:


From: Mike Tighe mti...@trudeauinstitute.org
Subject: [Histonet] Tissue processor
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, October 4, 2011, 11:56 AM


I am looking to purchase a Processor and embedding center. I have received a 
quote for VIP6 and a TEC5 but they seem  to be a bit out of our price range. 
Does anyone have another Processor/embedding center that they found to be 
reliable?

Thanks
Mike





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RE: [Histonet] Tissue processor

2011-10-04 Thread Breeden, Sara 
Although I appreciate individual opinions, do not overlook the Leica
line.  Us old timers may recognize the names Sakura or Thermo (in
its many permutations) more readily, I'd put my money on Leica every
time (and I have - with processor, embedding center, microtome and
autostainer).  They do not let me down.  I have no issues with either
other vendor, but I placed my trust in German engineering.  I'm just
sayin'...

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RE: [Histonet] Tissue processor

2011-10-04 Thread Marcum, Pamela A 
We are just getting our second Excelsior and love them.  It cuts tech time 
changing bottles and paraffin as well as keeping us on track daily for changes 
and rotations.  We have to VIP, that are very old and one just died.  That is 
the one we are replacing with Thermo.  The VIPs are great units just could not 
go the price and reagent exposure with them. We also have Leica ASP300 love but 
skipped it for the same reasons.

Pam Marcum 
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
Sent: Tuesday, October 04, 2011 12:08 PM
To: Mike Tighe; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue processor

We purchased a processor this past year and went with the VIP6. We demo'd
several but went with the VIP because of its reliability and quality. We own 
two older models, the oldest over 20 years old, that still run well. We also 
liked the Leica model that was slightly less, but I was able to convince 
administration the VIP was worth the difference.
 
Hope this helps.

Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
 

From: Mike Tighe mti...@trudeauinstitute.org
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, October 4, 2011 11:56 AM
Subject: [Histonet] Tissue processor

I am looking to purchase a Processor and embedding center. I have received a 
quote for VIP6 and a TEC5 but they seem  to be a bit out of our price range. 
Does anyone have another Processor/embedding center that they found to be 
reliable?

Thanks
Mike





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RE: [Histonet] Tissue Processor Reagents

2011-01-04 Thread sgoebel 
I think the standard rule of thumb is 700-800 blocks?

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Katherine Leonard
Sent: Tuesday, January 04, 2011 12:32 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Reagents

Hello histonetters ~

Is there any consensus regarding how often to change processor reagents?
I
am using the TBS ATP1 tissue processor and the tissue size ranges from
biopsy to 1.5cm^3.

-- 
Katherine Leonard
Research Associate
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RE: [Histonet] Tissue Processor Advice

2010-10-22 Thread Feher, Stephen 
We are using the Peloris with a 2 hr, 4 hr and 8 hr protocol.  We run 2
hour protocols throughout the day with an average of 4-5 runs per day
depending on specimen volume.  We really like this processor.  We have
had them for 10 months now, are using factory protocols and have not had
any specimens that have been either under or over processed.  The techs
and the pathologists are very pleased with it. 


Steve

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
caymanfl...@gmail.com
Sent: Friday, October 22, 2010 4:17 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Advice

We are in need of some advice regarding rapid tissue processors.  Models
we are considering:

Sakura Xpress
Leica Peloris
Thermo STP 420

It seems none of these models are perfect in every respect.  I'm
interested in anyone's opinions of these processors and your experience
with them.

All input is appreciated!
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RE: [Histonet] Tissue Processor Advice

2010-10-22 Thread Jesus Ellin 
How are you meeting the hours of fixation requirement for Breast?  With
2 and 4 and 8 hours,, But recently there are articles calling for Her 2
to be done on GI cases.  Just want to know you insight on this?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher,
Stephen
Sent: Friday, October 22, 2010 2:12 PM
To: caymanfl...@gmail.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Tissue Processor Advice

We are using the Peloris with a 2 hr, 4 hr and 8 hr protocol.  We run 2
hour protocols throughout the day with an average of 4-5 runs per day
depending on specimen volume.  We really like this processor.  We have
had them for 10 months now, are using factory protocols and have not had
any specimens that have been either under or over processed.  The techs
and the pathologists are very pleased with it. 


Steve

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
caymanfl...@gmail.com
Sent: Friday, October 22, 2010 4:17 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Advice

We are in need of some advice regarding rapid tissue processors.  Models
we are considering:

Sakura Xpress
Leica Peloris
Thermo STP 420

It seems none of these models are perfect in every respect.  I'm
interested in anyone's opinions of these processors and your experience
with them.

All input is appreciated!
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RE: [Histonet] Tissue Processor Advice

2010-10-22 Thread WILLIAM DESALVO 

There are so many good to great processors on the market, but all have their 
plus and minus issues. You really have to decide what your two or three most 
important issues will be and then rank them. With the trend in becoming more 
efficient/cost effective, reducing TAT and LEAN process improvement, I suggest 
you look to improve your process to match these trends and you will be lead to 
rapid tissue processing in a LEAN way. Couple the previously mentioned 
trends/issues with versatility of processing with your routine formalin fixed 
samples with molecular fixed samples on the same instrument, I suggest the 
Sakura Xpress (X50 or X120) rapid processors.
 
These instruments provide continuous loading, small batch and require a small 
volume of reagent for processing and then discard. The instruments do have a 
required reagent kit and there is a variable pre-processing protocol, depending 
on the tissue type and fat content. Using the reagent kit does allow for cost 
savings over conventional processing and I find the pre-processing allows for 
better precision processing techniques and protocols, we have never over 
processed tissues. Another great advantage is the increased velocity of the 
workflow as the instruments are continuous load (no cleaning cycle between 
batches) and small batch (1 to 40 cassettes). Loading 1 or 2 cassettes when a 
STAT or RUSH cases arrives and completes fixation does not interrupt the 
process or require special handling. An important factor to consider is that 
continuous load processing does assist in workload leveling, which can assist 
in reducing employee stress, increase productivity and error reduction. All 
these things lead directly to reduced TAT. Add the often overlooked advantage 
of removing Xylene from your tissue processing, and again, I suggest you 
consider the Xpress.
 
I was an early adopter (5+ yrs use) and continue to use the X120 (2 units). I 
have not experienced any instrument performance or maintenance issues. I have 
had three software upgrades and the instruments had to go down for several 
hours to install the upgrade. The X120 and now the X50 have two programming 
options 1+ hour or 2+ hour processing. The most LEAN factor is that after the 
first basket of up to 40 cassettes, the next one comes off 20 or 40 minutes 
later and you can continuously load. There is no other instrument that can 
allow you to process in as small as batch or provide the continuous delivery of 
cassettes. You can do rapid processing with all of the instruments you are 
considering, but conventional, one reaction chamber instruments will limit the 
number of times the instrument can be run each day and that increases the batch 
size.
 
Rapid processing does demand change in the way your lab does it's work. The 
first is standardization of gross dissection to 3 mm thickness. It does not 
matter the instrument, if you want to truly move into rapid processing, then 
you have to standardize your process in the gross room. The process of 
retraining and standardizing your gross room is well worth the effort. Another 
issue that you have to fully consider is how will specific fixation times 
affect your workflow. We now have specific guidelines for ER/PgR; Her2 and if 
you follow the NCI protocols for cancer tumors, you have to record your actual 
fixation times. Trying to manage all the different fixation times becomes 
difficult and will slow down your process. If you use your tissue processor to 
complete fixation, the processor will force you into larger batches. When you 
have to wait for tissues to complete fixation before starting your processing 
program, you also limit the number of times the processor can be run in a day. 
I find that separating fixation from processing is the best approach. You 
process specimens only when the optimal fixation time has been completed. Stop 
and consider all the different tissue types, size, fat content and required 
protocols, and you will see the value of a rapid processor that has small batch 
and continuous load capabilities. Meet all your fixation requirements and needs 
and only use the tissue processor for processing, not fixation. This is very 
LEAN concept and a concept that I believe you will need to embrace.

Whenever you have the opportunity to change your process, I always suggest you 
look to improve the process, use the latest process improvement techniques and 
select an instrument that will assist in the change and prepare you for future 
change. My philosophy is we cannot continue to do tomorrow what we do today and 
expect a different outcome or result. Just my thoughts and experience, I hope 
this will help you. Do not hesitate to contact me if you have any questions.

William DeSalvo, B.S., HTL(ASCP)
 

 Date: Fri, 22 Oct 2010 16:16:50 -0400
 From: caymanfl...@gmail.com
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Tissue Processor Advice
 
 We are in need of some advice regarding rapid tissue

Re: [Histonet] Tissue Processor Advice

2009-08-05 Thread Anne van Binsbergen 
Sakura Tissue Tek VIP5!!
I have 2 of these sturdy workhorses and am very happy
Have been associated with VIP5's for as long as they have been around -
wonderful machines!
AnnieinAbuDhabi
aka.Annie out of Africa
2009/8/5 Igor Deyneko igor.deyn...@gmail.com

 Dear Histonetters!
 I need your advice in Tissue Processors. The one we currently use, Tissue
 Tek VIP 3000, is archaic and has finally died. So we are looking into
 buying
 a new one. I know that Thermo and Leica both have processors, as well as
 new
 Tissue teks, but I wanted to get opinions if you have a preference of a
 machine, pros and cons of each. I mostly process tumors, with occasional
 mouse organs thrown in.
 Any suggestions will be very helpful!
 Thank you in advance.
 Sincerely,
 Igor Deyneko
 Infinity Pharmaceuticals
 Cambridge, MA
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-- 
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
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Re: [Histonet] Tissue Processor Advice

2009-08-04 Thread thecitan 
I recommend the new tissue tech.  I use it in my lab and its reliable and 
intuitive.

I strongly warn against TBS. I use one in my other lab and its been nothing but 
trouble. Its chemical storage is unreliable and leads to cross contamination.  
--Original Message--
From: Igor Deyneko
Sender: histonet-boun...@lists.utsouthwestern.edu
To: Histonet
Subject: [Histonet] Tissue Processor Advice
Sent: Aug 4, 2009 1:43 PM

Dear Histonetters!
I need your advice in Tissue Processors. The one we currently use, Tissue
Tek VIP 3000, is archaic and has finally died. So we are looking into buying
a new one. I know that Thermo and Leica both have processors, as well as new
Tissue teks, but I wanted to get opinions if you have a preference of a
machine, pros and cons of each. I mostly process tumors, with occasional
mouse organs thrown in.
Any suggestions will be very helpful!
Thank you in advance.
Sincerely,
Igor Deyneko
Infinity Pharmaceuticals
Cambridge, MA
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