Re: [Histonet] Macrophage Stain

[Julio Benavides via Histonet]

Hi Jessica,

there are actually quite a number of Abs working on sheep tissues. Take a
look at this paper, most of them work in sheep (I believe all but
gamm-IFN). Let me know if you cannot access the paper and I will send you a
copy.

https://www.ncbi.nlm.nih.gov/pubmed/27315822

Happy to comment on the IHC conditions if you have any doubt.

Cheers

Julio

Jessica Riggleman via Histonet 
escribió:


Hello,

Could you please help me find a macrophage stain that will work with
sheep (specifically sheep laminectomy sections)? We are thinking
RAM-11 or HAM-56, but unsure.

Also, what about Rabbit PLF?

Please note both are embedded in mega cassettes for paraffin processing.

Thank you,
Jessica



_

Jessica Riggleman | Research Associate

Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:

Confidentiality Note:  This email is confidential and intended solely
for the use of the individual to whom it is addressed. If you are not
the intended recipient, be advised that you have received this email
in error and that any use, dissemination, forwarding, printing, or
copying of this email is strictly prohibited. If you have received
this email in error please contact the sender. Any views or opinions
presented are solely those of the author and do not necessarily
represent those of Globus Medical, Inc. Although this email and any
attachments are believed to be free of any virus or other defects
which might affect any computer or IT system into which they are
received, no responsibility is accepted by Globus Medical, Inc. for
any loss or damage arising in any way from the receipt or use thereof.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Macrophage Stain

[Elizabeth Chlipala via Histonet]

Jessica

We have not tried Ram-11 in sheep it's a macrophage antibody against rabbit, in 
our hands it did not cross react with human, cyno or rat.  We use MAC387 to 
stain macrophages in sheep.  It's not entirely specific to macrophages but it 
is what we have used in the past.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-Original Message-
From: Jessica Riggleman via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, October 18, 2016 11:08 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Macrophage Stain

Hello,

Could you please help me find a macrophage stain that will work with sheep 
(specifically sheep laminectomy sections)? We are thinking RAM-11 or HAM-56, 
but unsure.

Also, what about Rabbit PLF?

Please note both are embedded in mega cassettes for paraffin processing.

Thank you,
Jessica



_

Jessica Riggleman | Research Associate

Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:

Confidentiality Note:  This email is confidential and intended solely for the 
use of the individual to whom it is addressed. If you are not the intended 
recipient, be advised that you have received this email in error and that any 
use, dissemination, forwarding, printing, or copying of this email is strictly 
prohibited. If you have received this email in error please contact the sender. 
Any views or opinions presented are solely those of the author and do not 
necessarily represent those of Globus Medical, Inc. Although this email and any 
attachments are believed to be free of any virus or other defects which might 
affect any computer or IT system into which they are received, no 
responsibility is accepted by Globus Medical, Inc. for any loss or damage 
arising in any way from the receipt or use thereof.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet