Re: the absorbance value 1.05 at 260/280 of protein solution

It means you have to get a good text book on spectrophotometry and read it. That will give you a good idea of what exactly it means. :) Good luck! Pow On 28 October 2015 at 07:13, Sudheer Sangeetham wrote: > Hello people > > What does it mean by the absorbance

Re: Whole cell extract gel samples for western blot

Hi Paul, I used to use a large bore needle (~18-19 guage) and pass the sample through it a few time (more like 5-10 times); next use a small bore needle (~23 guage) to pass the sample another 5-10 times. Spin the sample at high speed (~10,000-12,000 rpm in a small Kubota or Eppendorf centrifuge -

Re: Plasmid mix in the clone - the mechanism?

On 24 April 2013 12:55, Vladimir Gainullin gainul...@gmail.com wrote: DpnI digest? On Wed, Apr 24, 2013 at 12:39 AM, DK d...@no.email.thankstospam.net wrote: Wonder if anyone has good explanation as to how this is occuring: Once in a while, looking at sequences of mutant clones

Re: RNA isolation from Formaldehyde fixed samples

On 17 April 2013 14:03, Pow Joshi pow.jo...@gmail.com wrote: On 17 April 2013 10:54, Nick Theodorakis nick.theodora...@gmail.comwrote: On Tuesday, April 16, 2013 12:48:56 PM UTC-4, Pow Joshi wrote: On 16 April 2013 00:00, DK d...@no.email.thankstospam.net wrote: In article mailman

Fwd: RNA isolation from Formaldehyde fixed samples

On 16 April 2013 00:00, DK d...@no.email.thankstospam.net wrote: In article mailman.259.1366062468.10461.meth...@net.bio.net, Pow Joshi pow.jo...@gmail.com wrote: Dear All, I have some cells fixed in 4% formalin (neutral I believe). I was wondering if anyone has tried any RNA extraction

RNA isolation from Formaldehyde fixed samples

Dear All, I have some cells fixed in 4% formalin (neutral I believe). I was wondering if anyone has tried any RNA extraction on such cell/tissue samples, and if you have any wisdom including kits, tricks, solutions, problems that you'd share with me. I would appreciate it hugely. Thank you much,

Re: Homebrew Dark Reader amber screen?

On 11 December 2012 23:18, WS novalidaddr...@nurfuerspam.de wrote: Dear colleagues, I could grab a dark reader transilluminator (a box with a blue filter that is using filtered visible light instead of UV to illuminate your EtBr stained DNA gels), but without the amber filter. Instead of

Re: Some funny (?) comics

On 3 May 2012 19:12, DK d...@no.email.thankstospam.net wrote: In article mailman.257.1336063765.3456.meth...@net.bio.net, Cathal Garvey cathalgar...@gmail.com wrote: Can also recommend the following to science nerds: www.smbc.com www.xkcd.com Thanks! these are different from PhD :)

Re: Ammonium sulfate precipitation from solutions with high sugar content

well, here's a plant phloem/Xylem sap protein isolation I found http://www.springerlink.com/content/h8622j4747246137/#section=82175page=4locus=23 They use Centricon ultra filtration step to remove the sugars it seems to me followed by TCA/acetone precipitation On 1 May 2012 07:18,

Re: 10X PBS

I like this discussion. A lot of info about old forgotten physical chem :) Thank you DK, Engelbert, Jayakumar, and others. On 9 January 2012 03:08, DK d...@no.email.thankstospam.net wrote: In article mpg.29740567a625f636989...@news.individual.de, Dr Engelbert Buxbaum

Re: Hello...Native Page is not polymerising

On 21 October 2011 05:01, Christian Praetorius p...@gmx.net wrote: sudheer sangeetham sudheer.pb...@gmail.com wrote: Could any please tell me why my Native PAGE is not polymerizing. I have referred one article, which i mentioned below and did in similar way and same concentrations but didnt

Re: Why bl-21 use as protein exression strain?

you can find the genotype on any of the commercial websites from where you buy the strain: for BL21 (DE3) it is: F–, *omp*T, *hsd*SB(rB-, mB-), *dcm*, *gal*, λ(DE3) *For *BL21(DE3) pLysS*F–, *omp*T, *hsd*SB(rB-, mB-), *dcm*, *gal,* λ(DE3), pLysS (Cmr) Pow On 14 March 2011 18:54, sudheer

Re: Sealing Tape for Petri Dishes?

On 27 August 2010 15:21, WS novalidaddr...@nurfuerspam.de wrote: Dear colleagues, many thanks for your quick and numerous replies! I am familiar with parafilm for storing bacterial plates, but for a product that is to be commercialized, it does not look noble enough and, more important, it

Re: Western blot trouble

On 30 January 2010 00:57, Scott Brown sbr...@ccia.unsw.edu.au wrote: Hi all, I've purchased a santa cruz Ab which is usually not such a great thing in my experience, i optimized it using a wt cell line and a cell line that over expressed my protein of interest. The blot looked great.