Dear all,
I have had problems using benzonase nuclease to remove DNA from bacterial (E.
coli) lysates, from which I am purifying a ssDNA-binding protein. The problem
is, that I want to purify a DNA-free protein, and benzonase did work for that,
but then of course I need to remove the benzonase
I think this will be an unusual question. I use a Biologic Duoflow
chromatography system (from Bio-Rad) for a lot of protein purification, and
lately I have had a bizarre problem with the system. The pumps have been
starting by themselves, even in front of my eyes as I watch, and every time
Hello everyone,
I am looking for an expression vector for GST-tagged TEV protease, and after
searching through everything on Addgene and ATCC, I am coming up blank. All I
can find are vectors for MBP and His-tagged TEV protease. Google doesn't help
much either, it keeps leading me to
I have a quick question for everyone: When using glutathione Sepharose (4B Fast
Flow from GE) to purify a GST-tagged protein, has anyone used it at pH 6.5 and
have it work? I ask because I am trying to purify a DNA-binding protein that
is greatly aggregated after the initial glutathione
You can use the A260/A280 as an indicator of protein purity. Ideally, pure
protein should have an A260/A280 value of 0.6, so 1.05 is very high, and
indicates that you have nucleic acid contamination (the A260 component is too
high).
From:
Here is a quick question:
Has anyone ever heard of poly-deoxyribose phosphate (i.e. a bare DNA
sugar-phosphate backbone without any bases) being commercially available? I
have not been able to find any source for it, and I have made some enquiries,
but wondered if anyone else has ever hard of
