Re: [spctools-discuss] How to process MS3 Data from Orbitrap Fusion?

2014-10-10 Thread Will Fondrie
Problem solved, it was just a silly mistake on my part. It turns out my system's PATH pointed to an older version of msconvert. I changed that and now it is working perfectly. Best, Will On Tuesday, October 7, 2014 2:49:04 PM UTC-4, Will Fondrie wrote: > > Thank you Jimmy, > > I'll submit my pr

Re: [spctools-discuss] How to process MS3 Data from Orbitrap Fusion?

2014-10-07 Thread Will Fondrie
Thank you Jimmy, I'll submit my problem to the msconvert support list. When I hear back, I'll make sure to update here with the answer as well. I'm glad to know that it won't be a problem searching with Comet. Best, Will On Friday, October 3, 2014 1:14:23 PM UTC-4, Jimmy Eng wrote: > > To sear

Re: [spctools-discuss] How to process MS3 Data from Orbitrap Fusion?

2014-10-03 Thread Jimmy Eng
To search MS3 spectra in Comet, you need to set the parameter "ms_level = 3". Typically the value of that parameter is set to "2" for MS/MS scans. http://comet-ms.sourceforge.net/parameters/parameters_201402/ms_level.php Although a few of the ProteoWizard devs are here too, you might consider

[spctools-discuss] How to process MS3 Data from Orbitrap Fusion?

2014-10-03 Thread Will Fondrie
Hi all, We have a RAW file that was collected using MS3 on an Orbitrap Fusion, and I have a couple questions about processing it. First, what is the best way to convert the RAW file to mzML, so that it can be searched with Comet or one of the other TPP search engines? I've tried using the late