Re: [spctools-discuss] Select only top matching protein in spectrast

2020-12-08 Thread shubha...@gmail.com
Dear David, Thanks for explaining the principle of multiple match. I was asking if there is any flag in spectraST that returns only top match. eg Protein= 1/NP_001447.2|gn|FLNA:2316| instead of all four

Re: [spctools-discuss] Select only top matching protein in spectrast

2020-12-08 Thread 'David Shteynberg' via spctools-discuss
Hi Shubham, Thanks for using the TPP tools and submitting your question here. A peptide may map to more than one protein, when this happens the TPP will usually map each peptide to all proteins where it can occur. There are some exceptions to this such as I/L substitutions and protein specific

[spctools-discuss] Select only top matching protein in spectrast

2020-12-08 Thread shubha...@gmail.com
Hi, I am using RefSeq database which has isoforms. My pipeline includes Mascot, peptide prophet, iProphet and spectraST. In the spectraST output .sptxt file, for some spectra there are multiple proteins identified. eg.