Re: [spctools-discuss] When to use "Decoy hits to pin down negative distribution" in PeptideProphet

2021-01-20 Thread Soroush F
Dear David, Thanks so much for your time answering my questions. I gather from your answer that this option is for TPP. If that is the case, why does the PeptideProphet probabilities changes when selecting the option? Am I missing something? Many thanks. Soroush On Tuesday, January 19, 2021

Re: [spctools-discuss] When to use "Decoy hits to pin down negative distribution" in PeptideProphet

2021-01-20 Thread 'David Shteynberg' via spctools-discuss
This option changes the way mixture models in the TPP (PeptideProphet) are generated. The probabilities are estimated based on the mixture models learned by PeptideProphet. When the mixture models change so will the probabilities. On Wed, Jan 20, 2021 at 8:44 PM Soroush F wrote: > Dear David,

Re: [spctools-discuss] Error generation while converting raw files

2021-01-20 Thread 'David Shteynberg' via spctools-discuss
I am not sure I know a tool called "ProptideProphet" ;) Perhaps you tried to run PeptideProphet but it generated no results for you? When you run "Analyze Peptides" in the TPP interface, it should create a file called interact.pep.xml by default, that will contain probabilities among other

Re: [spctools-discuss] Error generation while converting raw files

2021-01-20 Thread giangiacomo beretta
Hi David! It works nicely, thank you ! Now I am experiencing another issue of mine :) I run ProptideProphet on the XML fiel generated by XTandem. I need this file presuming that PP will add probabilities to the identified peptides as this is requested by SpectraST to generate the