[spctools-discuss] Re: The problem with running xinteract on OMSSA pepxml

2009-06-30 Thread Ping
Is this something else and suppose to be a new post? YP On Jun 30, 11:08 am, "Zhi Sun" wrote: > Hi all, > > I just checked in two programs, and did not put the log message. > > I will give a short description here: > > createChargeFile.pl: this program reads the ms2 file generated by CPM > (cha

[spctools-discuss] Re: building tpp from svn (rev 4395) on ubuntu 9.04

2009-06-30 Thread Brian Pratt
Can you be more specific? While some files have changed, and some have been added, the source code directory structure is pretty much the same. Brian -Original Message- From: spctools-discuss@googlegroups.com [mailto:spctools-disc...@googlegroups.com] On Behalf Of Andreas Quandt Sent: T

[spctools-discuss] Re: building tpp from svn (rev 4395) on ubuntu 9.04

2009-06-30 Thread Andreas Quandt
Hey Natalie, hello Brian Sorry for getting back to you so late. I tried the compilation and it worked like a charm :-) I am only a little bit confused about the structure as it completely differs from the stable branch. Hence I am not exactly sure how to best integrate the trunk in an existin

[spctools-discuss] Re: iProphet Information

2009-06-30 Thread Jake W
David-- Thanks. That's how I've been using it and the results have looked really good. Jake --~--~-~--~~~---~--~~ You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to spctools-di

[spctools-discuss] Re: iProphet Information

2009-06-30 Thread David Shteynberg
Hi Jake, It is up to you to define the experiment boundaries. I would imagine that different fractions of SCX of a single sample, for instance, can be classified as one experiment. As long as different fractions or separate physical runs have different MS data file names there will be no proble

[spctools-discuss] Re: iProphet Information

2009-06-30 Thread Jake W
David-- Thanks for your reply. Just to clarify: if one wants to use iProphet to combine physically separate analyses (eg., multiple SCX fractions or gel slices, with a separate MS/MS data file for each) from a single biological 'experiment'/sample, should the -E label be used to differentiate ea

[spctools-discuss] Re: combining X!Tandem / SEQUEST / MASQOT etc

2009-06-30 Thread David Shteynberg
Hi Christine, We do have a tool in the TPP, called iProphet, for combining search engines. This tool applies a set of 5 statistical models of which the search engine combining is merely one. We will have a paper coming out on iProphet in the near future. Meanwhile, the tool is available to try

[spctools-discuss] Re: iProphet Information

2009-06-30 Thread Greg Bowersock
Thanks, I'll have to play around with it some to see how I like it. I do combine search engines, so it'll be interesting to compare the difference. On Tue, Jun 30, 2009 at 1:41 PM, David Shteynberg < dshteynb...@systemsbiology.org> wrote: > > One mistake to run ProteinProphet on iProphet dat

[spctools-discuss] Re: iProphet Information

2009-06-30 Thread David Shteynberg
One mistake to run ProteinProphet on iProphet data you must use the IPROPHET option as follows: > ProteinProphet interact.iproph.pep.xml interact.iproph.prot.xml IPROPHET On Tue, Jun 30, 2009 at 11:39 AM, David Shteynberg wrote: > Hi Greg, > > The iProphet powerful tool that is very simple

[spctools-discuss] Re: iProphet Information

2009-06-30 Thread David Shteynberg
Hi Greg, The iProphet powerful tool that is very simple to run. Unless you are testing the performance of the various models iProphet applies, there is not need to set iProphet parameters. The easiest way to run the tool is on a single analysis (one experiment, one search engine), this only req

[spctools-discuss] Re: The problem with running xinteract on OMSSA pepxml

2009-06-30 Thread Zhi Sun
Hi all, I just checked in two programs, and did not put the log message. I will give a short description here: createChargeFile.pl: this program reads the ms2 file generated by CPM (charge prediction program), or directory which contains a list of dta file, and creates a .charge file. The .cha

[spctools-discuss] The problem with running xinteract on OMSSA pepxml

2009-06-30 Thread Ping
Hi, I am trying to run the xinteract on the omssa pep.xml output files. my omssa's version is 2.1.4, my TPP version is 4.2.1. But I couldn't get it through. I search the old post, there is a similar post, but the problem was solved by specifying enzyme to xinteract. I tried it, but is still not

[spctools-discuss] Re: iProphet Information

2009-06-30 Thread Greg Bowersock
Just bumping this one up, since it looks like it got missed. On Jun 10, 12:08 pm, "bowers...@gmail.com" wrote: > I was wondering if someone could post some more detail on iProphet. > One thing that I would like to know is what all command line arguments > it takes, maybe some sample command line

[spctools-discuss] Re: X!Tandem Parameters File

2009-06-30 Thread Natalie Tasman
Hello Johannes, I hope that other people on the list will chime in with their advice, but to start things of, here is a link to the official X!Tandem reference on all available parameters and options: http://www.thegpm.org/TANDEM/api/index.html Note that some of these options may be specifi

[spctools-discuss] Re: Processing mzXML data

2009-06-30 Thread Natalie Tasman
Hello Petri, If you're interested in mzXML, I would recommend starting here: http://tools.proteomecenter.org/wiki/index.php?title=Formats:mzXML Regarding mzXML tools, you'll find information on data converters which take vendor-specific files and convert them to mzXML as well as other tools.

[spctools-discuss] Re: X!Tandem Parameters File

2009-06-30 Thread ZeiserMHH
Hello Oded, Hello James, Hello Group, could you please give me some advice how i can create a x!tandem parameters file? is there any way to do this except from changing the xml-code of the example files? thank you a lot for the help! Johannes On 26 Jun., 22:13, James Dowell wrote: > Oded, > >

[spctools-discuss] Protein groups derived from ProteinProphet

2009-06-30 Thread sarah gerster
Hello, I have some questions concerning protein groups derived from ProteinProphet. The questions seem to be independent of recent TPP versions (they arise as well with TPP v4.1 as with TPP v4.2). OS is linux. To illustrate my questions I have uploaded three XML extracts from two different experi

[spctools-discuss] Processing mzXML data

2009-06-30 Thread Petri
Hi I would like to know what processing options I have for mzXML data format in TPP. I tried to look for this from http://sourceforge.net/projects/sashimi/ but it didn't catch my eye. Could somebody with more experience point me to a right direction or even better, tell me? Thank you, -Petri