[spctools-discuss] Re: No retention time details in Tandem pepXML?

2012-08-08 Thread magnus.palmb...@gmail.com
Dear All, We have a similar problem in that we don't see any retention times (they are all zero) from the X!Tandem+Tandem2XML-generated pepXML files. This problem appeared with the 4.6 version of the TPP with the updated X!Tandem, previously we had no problems with it. For SpectraST we still

[spctools-discuss] Creating / compiling a mzXML file from scratch - C# code available ?

2012-08-08 Thread wlstraube
Hello TPP-Team, I would like to compile a mzXML file from a Thermo raw-file, which has an internal structure that cannot be converted by msconvert. I am able to read out the raw-file and process it, but now want to save it as a mzXML. Therefore, without reinventing the wheel, I would like to

[spctools-discuss] Re: Proteome Discoverer's .msf to TPP input files (pepXML)

2012-08-08 Thread patrick Halvey
Hello. I came across this post while doing a google search for conversion of msf files to pepXML. I am trying to use Proteome Discoverer output with a protein assembly tool called IDpicker, but of course it is impossible to export pepXML format from Proteome Discoverer. Has there been a

Re: [spctools-discuss] Creating / compiling a mzXML file from scratch - C# code available ?

2012-08-08 Thread Brian Pratt
The best thing would be to just use your new reader code to extend msconvert so that it *does* read your file. Then you can output to anything msconvert already handles. - Brian Pratt On Wed, Aug 8, 2012 at 8:40 AM, wlstraube stra...@imp.ac.at wrote: Hello TPP-Team, I would like to compile a

Re: [spctools-discuss] Creating / compiling a mzXML file from scratch - C# code available ?

2012-08-08 Thread wlstraube
Dear Brian, Thank you very much for the quick response. If I get you right, I should take the msconvert source code and implement our code. This sounds logically, but for a first try I would try to keep it simple. I would need only the part which writes down the required parameters. Would

Re: [spctools-discuss] Creating / compiling a mzXML file from scratch - C# code available ?

2012-08-08 Thread Brian Pratt
For simplicity, you would probably want to go with the hacking up the old readw code. But if you have any plans for sharing your work or using it very far into the future then you're better off with ProteoWizard and msconvert since that gets maintained and offers more output formats. Brian On

[spctools-discuss] Re: Creating / compiling a mzXML file from scratch - C# code available ?

2012-08-08 Thread kshitiz
Hiya, What about using ReAdW ? This is what we routinely use to convert RAW files to mzXML. Cheers, Kshitiz On Wednesday, August 8, 2012 4:40:47 PM UTC+1, wlstraube wrote: Hello TPP-Team, I would like to compile a mzXML file from a Thermo raw-file, which has an internal structure that

[spctools-discuss] Re: No retention time details in Tandem pepXML?

2012-08-08 Thread mhoopmann
Can you post what your RC number is? My test box over here using 4.6 RC9 rev 0 correctly displays the retention times with PepXML Viewer under the retention_time_sec column. Thanks, Mike On Wednesday, August 8, 2012 6:42:05 AM UTC-7, magnus@gmail.com wrote: Dear All, We have a similar