Re: [spctools-discuss] error analyzing InSpecT results (pepxml) with PeptideProphet

2010-02-11 Thread David Shteynberg
Hi Christian, The problem is here: WARNING: Inspect only support semi-parametric PeptideProphet modelling, which relies on a DECOY search. You need to include Decoy sequences (with a decoy tag) in the protein database you are searching and specify this to PeptideProphet. If you are using

[spctools-discuss] error analyzing InSpecT results (pepxml) with PeptideProphet

2010-02-09 Thread Cristian
Dear All, I am trying to analyze InSpecT results on pepxml format with TPP. I copied below the error message that TPP generates. Any help will be greatly appreciated. Cheers, Cristian run_in c:/Inetpub/wwwroot/ISB/data/try; c:\Inetpub\tpp-bin\xinteract -Ninteract0209.pep.xml -p0.05 -l7 -O